Abstract
Viable microorganisms were found in Miocene lacustrine clays of the cypris formation excavated from 200-m below the surface as spoil during open-cast brown coal mining (Sokolov Brown Coal Basin, North-Western Bohemia, Czech Republic). Both saprotrophic microfungi of the genera Penicillium, Verticillium, Cladosporium and Aspergillus as well as heterotrophic bacteria were isolated from an intact sediment cores. Heterotrophic bacteria were classified by the MIS Sherlock System as representatives of genera Nocardiopsis, Arthrobacter, Micrococcus, Kocuria, Rothia, Clavibacter, Bacillus, Paenibacillus, Brevibacillus, Microbacterium, Acinetobacter and Pseudomonas. A bacterium found among the strains had an atypical fatty acids profile enriched by branched fatty acids and polyunsaturated fatty acid (18:3\(\omega\)6) and gave no MIS Sherlock match. Phospholipid fatty acids analysis indicates a relatively high (535 pmol g−1) but inhomogeneously distributed viable microbial biomass. Fatty acids analyses of non-fractioned lipids (representing viable, storage and dead biomass; 8390 pmol g−1) detected rich and homogenous profiles with fungal, bacterial and actinomycetal markers but no protozoan and algal fatty acids markers.
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Acknowledgements
This work was founded through projects 526/03/1259 from the Czech Science Foundation, S600660505 from Czech Academy of Sciences and AV 0Z 606 605 21 Research Plan of ISB AS CR. Authors thank to Mr. Patrick Hill for language revision.
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Elhottová, D., Krištůfek, V., Frouz, J. et al. Screening for microbial markers in Miocene sediment exposed during open-cast brown coal mining. Antonie Van Leeuwenhoek 89, 459–463 (2006). https://doi.org/10.1007/s10482-005-9044-8
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DOI: https://doi.org/10.1007/s10482-005-9044-8