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Thioclava indica sp. nov., isolated from surface seawater of the Indian Ocean

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Abstract

A short-rod shaped, non-motile and Gram-negative strain, designated as DT23-4T, was isolated from a dotriacontane-degrading bacterial consortium, enriched in the surface seawater of the Indian Ocean. To delineate its taxonomic position, strain DT23-4T was subjected to polyphasic characterization. Strain DT23-4T can grow at temperatures from 10 to 41 °C, in the range of pH 6–10 and at salinities from 0 to 18 % (w/v). It is positive for denitrification, oxidase and catalase, but negative for gelatin hydrolysis and indole production. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain DT23-4T affiliates to the genus Thioclava, sharing high sequence similarity to Thioclava dalianensis DLFJ1-1T (98.2 %), Thioclava atlantica 13D2W-2T (96.8 %), Thioclava pacifica TL 2T (95.9 %) and less than 95.7 % with other species of the family Rhodobacteraceae. The digital DNA:DNA hybridization (dDDH) pairwise values between strain DT23-4T and T. dalianensis DLFJ1-1T, T. atlantica 13D2W-2T and T. pacifica TL 2T were 21.5 ± 2.3, 20.6 ± 2.3 and 20.9 ± 2.3 %, respectively. Similarly, the average nucleotide identity values between strain DT23-4T and these three type strains were 78.0, 77.1 and 77.3 %, respectively. The principal fatty acid was identified as Summed Feature 8 (C18:1 ω7c/ω6c) (81.9 %). The quinone of strain DT23-4T was identified as Q10 (100 %). The polar lipids of strain DT23-4T were found to contain phosphatidylethanolamine, phosphatidylglycerol, two glycolipids and four phospholipids. The genomic DNA G+C content was determined to be 60.3 mol%. On the basis of genotypic, phenotypic and chemotaxonomic analyses, strain DT23-4T can be concluded to represent a novel species of the genus Thioclava, for which the name Thioclava indica sp. nov. is proposed, with the type strain DT23-4T (= MCCC 1A00513T = LMG 27698T = KCTC 33533T).

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Fig. 1

Abbreviations

MCCC:

Marine culture collection of China

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Acknowledgments

This work was financially supported by COMRA program (No. DY125-15-R-01), National Program on Key Basic Research Project (973 Program) (No. 2012CB417300), and National Infrastructure of Microbial Resources of China (Nos. NIMR-2013-9; NIMR-2014-9).

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Correspondence to Zongze Shao.

Electronic supplementary material

Transmission electron micrographs of cells of strain DT23-4T and polar lipids of strain DT23-4T are available as supplementary figures in ANTO Online.

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Liu, Y., Lai, Q., Du, J. et al. Thioclava indica sp. nov., isolated from surface seawater of the Indian Ocean. Antonie van Leeuwenhoek 107, 297–304 (2015). https://doi.org/10.1007/s10482-014-0320-3

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