Ammonium, an end-product of catabolism, in low doses can promote adaptation of metabolic pathways in erythrocytes under conditions of extreme physical exercise. We compared the effects of two ammonium salts, ammonium chloride and ammonium carbonate, in two doses on biochemical parameters of rat erythrocytes 1 day after extreme physical exercise in a 4-week cycle of forced swimming. Of 16 analyzed parameters, the maximum number of significant shifts from the control was revealed in the groups of rats receiving ammonium chloride in doses of 20 and 10 mg/kg, and the minimal number of differences was found in groups treated with ammonium carbonate in the same doses. The comparison of the levels of reduced glutathione and 2.3-bisphosphoglicerate and activities of 5’-nucleotidase and Ca2+- and Na/K-ATPases attested to more rigorous control of the mechanism of oxygen delivery to tissues by erythrocytes after administration of ammonium chloride in a dose of 20 mg/kg.
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Baidyuk EV, Sobolev VE, Korf EA, Mindukshev IV, Krivchenko AI, Goncharov NV. Ultrastructural characteristics of slow and fast twitch muscle rat fibers after the cycle of forced swimming with administering of green tea extract and ammonium salts. Tsitologiya. 2019;61(9):740-749. Russian.
Goncharov NV, Mindukshev IV, Novozhilov AV, Korf EA, Tavrovskaya TV, Terpilovsky MA, Khmelevskoy DA, Skverchinskaya EA, Krivchenko AI. Adaptive biochemical changes of rat erythrocytes after the cycle of forced swimming with the use of green tea extract and ammonium preconditioning. Ross. Fiziol. Zh. 2018;104(12):1428-1441. Russian.
Cheng IS, Wang YW, Chen IF, Hsu GS, Hsueh CF, Chang CK. The Supplementation of branched-chain amino acids, arginine, and citrulline improves endurance exercise performance in two consecutive days. J. Sports Sci. Med. 2016;15(3):509-515.
Dasarathy S, Mookerjee RP, Rackayova V, Rangroo Thrane V, Vairappan B, Ott P, Rose CF. Ammonia toxicity: from head to toe? Metab. Brain Dis. 2017;32(2):529-538.
Goncharov N, Maevsky E, Voitenko N, Novozhilov A, Kubasov I, Jenkins R, Avdonin P. Nutraceuticals in sports activities and fatigue. Nutraceuticals: Efficacy, Safety and Toxicity. Gupta RC, ed. Amsterdam, 2016. Р. 177-188.
Hadzic M, Eckstein ML, Schugardt M. The impact of sodium bicarbonate on performance in response to exercise duration in athletes: a systematic review. J. Sports Sci. Med. 2019;18(2):271-281.
Hunter FR. Kinetic analysis of the permeability of human erythrocytes to NH4Cl. J. Gen. Physiol. 1968;51(4):579-587.
Leal Denis MF, Alvarez HA, Lauri N, Alvarez CL, Chara O, Schwarzbaum PJ. Dynamic regulation of cell volume and extracellular ATP of human erythrocytes. PLoS One. 2016;11(6). ID e0158305. doi: 10.1371/journal.pone.0158305
Leal JKF, Adjobo-Hermans MJW, Bosman GJCGM. Red blood cell homeostasis: mechanisms and effects of microvesicle generation in health and disease. Front. Physiol. 2018;9. ID 703. doi: 10.3389/fphys.2018.00703
Marginedas-Freixa I, Alvarez CL, Moras M, Leal Denis MF, Hattab C, Halle F, Bihel F, Mouro-Chanteloup I, Lefevre SD, Le Van Kim C, Schwarzbaum PJ, Ostuni MA. Human erythrocytes release ATP by a novel pathway involving VDAC oligomerization independent of pannexin-1. Sci. Rep. 2018;8(1). ID 11384. doi: 10.1038/s41598-018-29885-7
Musa-Aziz R, Chen LM, Pelletier MF, Boron WF. Relative CO2/NH3 selectivities of AQP1, AQP4, AQP5, AmtB, and RhAG. Proc. Natl Acad. Sci. USA. 2009;106(13):5406-5411.
Singh Patidar B, Meena A, Kumar M, Menon B, Rohil V, Kumar Bansal S. Adenosine metabolism in COPD: a study on adenosine levels, 5’-nucleotidase, adenosine deaminase and its isoenzymes activity in serum, lymphocytes and erythrocytes. COPD. 2018;15(6):559-571.
Wahl P, Zinner C, Yue Z, Bloch W, Mester J. Warming-up affects performance and lactate distribution between plasma and red blood cells. J. Sports Sci. Med. 2010;9(3):499-507.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 168, No. 10, pp. 425-429, October, 2019
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Novozhilov, A.V., Mindukshev, I.V., Korf, E.A. et al. Ammonium Salts Promote Functional Adaptation of Rat Erythrocytes on the Model of Forced Swimming. Bull Exp Biol Med 168, 444–448 (2020). https://doi.org/10.1007/s10517-020-04728-6
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DOI: https://doi.org/10.1007/s10517-020-04728-6