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MicroRNA-663 induction upon oxidative stress in cultured human fibroblasts depends on the chronological age of the donor

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Abstract

MicroRNAs, regulators of messenger RNA translation, have been observed to influence many physiological processes, amongst them the process of aging. Higher levels of microRNA-663 (miR-663) have previously been observed in human dermal fibroblasts subject to both replicative and stress-induced senescence compared to early passage cells. Also, higher levels of miR-663 have been found in memory T-cells and in human fibroblasts derived from older donors compared to younger donors. In previous studies we observed that dermal fibroblasts from donors of different chronological and biological age respond differentially to oxidative stress measured by markers of cellular senescence and apoptosis. In the present study we set out to study the association between miR-663 levels and chronological and biological age. Therefore we tested in a total of 92 human dermal fibroblast strains whether the levels of miR-663 in non-stressed and stressed conditions (fibroblasts were treated with 0.6 μM rotenone in stressed conditions) were different in young, middle aged and old donors and whether they were different in middle aged donors dependent on their biological age, as indicated by the propensity for familial longevity. In non-stressed conditions the level of miR-663 did not differ between donors of different age categories and was not dependent on biological age. Levels of miR-663 did not differ dependent on biological age in stressed conditions either. However, for different age categories the level of miR-663 in stressed conditions did differ: the level of miR-663 was higher at higher age categories. Also, the ratio of miR-663 induction upon stress was significantly higher in donors from older age categories. In conclusion, we present evidence for an association of miR-663 upon stress and chronological age.

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Acknowledgments

We would like to thank Corine de Koning-Treurniet, Joke Blom and Pim Dekker for their work in the laboratory. This work was supported by the Austrian Science Fund [P 24498-B20]; Genome Research Austria GEN-AU [Project 820982 „Non-coding RNAs”], grants by the Herzfelder’sche Familienstiftung to RGV and CE.R.I.E.S to JG, SenterNovem, IGE01014 and IGE5007, NGI/NWO; 05040202 and 050-060-810. The financial support by the Austrian Federal Ministry of Economy, Family and Youth and the National Foundation for Research, Technology and Development is gratefully acknowledged as well as financial support by Chanel Research and Technology.

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JG and RGV are co-founders of Evercyte GmbH.

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Correspondence to Johannes Grillari or Andrea B. Maier.

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Mariëtte E.C. Waaijer, Matthias Wieser, Johannes Grillari, and Andrea B. Maier have contributed equally.

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Waaijer, M.E.C., Wieser, M., Grillari-Voglauer, R. et al. MicroRNA-663 induction upon oxidative stress in cultured human fibroblasts depends on the chronological age of the donor. Biogerontology 15, 269–278 (2014). https://doi.org/10.1007/s10522-014-9496-1

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