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Paraoxonase 1 Polymorphisms Within a Mississippi USA Population as Possible Biomarkers of Enzyme Activities Associated With Disease Susceptibility

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Abstract

Paraoxonase (PON1) hydrolyzes paraoxon (PO) and diazoxon (DZO), active metabolites of insecticides parathion and diazinon. The PON1 gene has single nucleotide polymorphisms (SNPs) including a codon 192 arginine (R) to glutamine (Q) and methionine (M) to leucine (L) at codon 55. Hydrolysis of PO (POase), DZO (DZOase), dihydrocoumarin (lactonase), and phenyl acetate (arylesterase) were evaluated for associations with race, gender, age, and PON1 55/192 SNP genotypes. Variables were analyzed both individually and in combination. QQ individuals had higher lactonase (p < 0.001) than RR individuals. This might partially explain why predominantly RR African Americans have higher rates of coronary disease than predominantly QQ Caucasians. Significant (p < 0.001) differences in arylesterase were seen among genotypes with QQ and MM lowest whereas RR and LL were highest. This opposes the prevailing belief that arylesterase is unaffected by genotype and suggests that this activity cannot be used to quantify PON1 protein.

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Acknowledgments

This research was supported in part by National Institutes of Health ES015107. This is Center for Environmental Health Sciences publication number 129.

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Authors and Affiliations

  1. Basic Sciences Department and Center for Environmental Health Sciences, College of Veterinary Medicine, Mississippi State University, P.O. Box 6100, 240 Wise Center Drive, Mississippi State, MS, 39762-6100, USA

    Chiquita Y. McDaniel, Mary Beth Dail & Janice E. Chambers

  2. Pathobiology and Population Medicine Department and Center for Environmental Health Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, USA

    Robert W. Wills

  3. Entomology Unit, Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, MS, USA

    Howard W. Chambers

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  1. Chiquita Y. McDaniel
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  2. Mary Beth Dail
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Correspondence to Janice E. Chambers.

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McDaniel, C.Y., Dail, M.B., Wills, R.W. et al. Paraoxonase 1 Polymorphisms Within a Mississippi USA Population as Possible Biomarkers of Enzyme Activities Associated With Disease Susceptibility. Biochem Genet 52, 509–523 (2014). https://doi.org/10.1007/s10528-014-9663-8

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