Abstract
Batch fermentations for xylitol production were conducted using Candida boidinii (BCRC 21432), C. guilliermondii (BCRC 21549), C. tropicalis (BCRC 20520), C. utilis (BCRC 20334), and P. anomala (BCRC 21359) together with a mixture of sugars simulating lignocellulosic hydrolysates as the carbon source. C. tropicalis had the highest bioconversion yield (YP/S) of 0.79 g g−1 (g xylitol·g xylose−1) over 48 h. Additional fermentations with C. tropicalis achieved YP/S values of 0.6 and 0.39 g g−1 after 96 and 72 h using urea and soybean meal as the nitrogen sources, respectively. Ethanol and arabitol were also produced in all fermentation. Xylitol in the fermentation broth was recovered by cross-flow ultrafiltration. With prior application of 2 mg polydiallyl dimethylammonium chloride l−1 on the membrane surface, protein in the permeate was reduced from 7.1 to 1.5 mg l−1 after 2 h.
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Acknowledgements
We are grateful to the Industrial Development Bureau, Ministry of Economic Affairs, Taiwan for financial support for this work in the form of the 2005 Small Business Innovation Research Project No. 15.
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Ko, CH., Chiu, PC., Yang, CL. et al. Xylitol conversion by fermentation using five yeast strains and polyelectrolyte-assisted ultrafiltration. Biotechnol Lett 30, 81–86 (2008). https://doi.org/10.1007/s10529-007-9507-2
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DOI: https://doi.org/10.1007/s10529-007-9507-2