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Improved lycopene production by Blakeslea trispora with isopentenyl compounds and metabolic precursors

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Abstract

The highest lycopene production in mated cultures of Blakeslea trispora was 578 mg/l by adding 42 mg geraniol/l to the medium after 48 h of growth. The control gave 317 mg/l. Adding isopentenyl alcohol at 40 mg/l, mevalonic acid at 17.5 mg/l or dimethyl allyl alcohol at 150 mg, each after 36 h growth, gave lycopene yields 62, 45 and 47%, respectively, higher than the control.

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References

  • Dandekar S, Modi VV, Jani UK (1980) Chemical regulators of carotenogenesis by Blakeslea trispora. Phytochemistry 19(5):795–798

    Article  CAS  Google Scholar 

  • Gessler NN, Sokolo AV, Belozerskaya TA (2002) Initial stages of trisporic acid synthesis in Blakeslea trispora. Appl Biochem Microbiol 38:536–543

    Article  CAS  Google Scholar 

  • Kim SW, Seo WT, Park YH (1997) Enhanced production of β-carotene from Blakeslea trispora with Span 20. Biotechnol Lett 19:561–562

    CAS  Google Scholar 

  • Krinsky NI, Johnson EJ (2005) Carotenoid actions and their relation to health and disease. Mol Aspects Med 26:459–516

    Article  PubMed  CAS  Google Scholar 

  • Larsson SC, Bergkvist L (2010) Carotenoids may reduce breast cancer risk in women. Eur J Cancer 46:1079–1085

    Article  PubMed  CAS  Google Scholar 

  • López-Nieto MJ, Costa J, Peiro E et al (2004) Biotechnological lycopene production by mated fermentation of Blakeslea trispora. Appl Microbiol Biotechnol 66:153–159

    Article  PubMed  Google Scholar 

  • Mehta BJ, Cerdá-Olmedo E (1999) Lycopene cyclization in Blakeslea trispora. Mycoscience 40:307–310

    Article  CAS  Google Scholar 

  • Sun Y, Yuan QP, Frank V (2007) Effect of two ergosterol biosynthesis inhibitors on lycopene production by Blakeslea trispora. Process Biochem 42(10):1460–1464

    Article  CAS  Google Scholar 

  • Xu F, Yuan QP, Dong HR (2006) Determination of lycopene and β-carotene by high performance liquid chromatography using sudan I as internal standard. J Chromatogr B 838:44–49

    Article  CAS  Google Scholar 

  • Xu F, Yuan QP, Zhu Y (2007) Improved production of lycopene and β-carotene by Blakeslea trispora with oxygen-vectors. Process Biochem 42:289–293

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors would like to acknowledge the National Natural Science Foundation of China (Nos. 20776009 and 20976009) and PHR (IHLB) for financial support.

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Authors and Affiliations

  1. State Key Laboratory of Chemical Resource Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, P.O. Box 75, 15 Beisanhuan East Road, Beijing, 100029, People’s Republic of China

    Yan-qiu Shi & Qi-peng Yuan

  2. Biotechnology Application Center, Beijing Polytechnic, Beijing, 100029, People’s Republic of China

    Xiu-lan Xin

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  1. Yan-qiu Shi
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  2. Xiu-lan Xin
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  3. Qi-peng Yuan
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Correspondence to Qi-peng Yuan.

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Shi, Yq., Xin, Xl. & Yuan, Qp. Improved lycopene production by Blakeslea trispora with isopentenyl compounds and metabolic precursors. Biotechnol Lett 34, 849–852 (2012). https://doi.org/10.1007/s10529-011-0839-6

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  • DOI: https://doi.org/10.1007/s10529-011-0839-6

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