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Biodegradation of methyl parathion and p-nitrophenol by a newly isolated Agrobacterium sp. strain Yw12

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Abstract

Strain Yw12, isolated from activated sludge, could completely degrade and utilize methyl parathion as the sole carbon, phosphorus and energy sources for growth in the basic salt media. It could also completely degrade and utilize p-nitrophenol as the sole carbon and energy sources for growth in the minimal salt media. Phenotypic features, physiological and biochemical characteristics, and phylogenetic analysis of 16S rRNA sequence showed that this strain belongs to the genus of Agrobacterium sp. Response surface methodology was used to optimize degradation conditions. Under its optimal degradation conditions, 50 mg l−1 MP was completely degraded within 2 h by strain Yw12 and the degradation product PNP was also completely degraded within 6 h. Furthermore, strain Yw12 could also degrade phoxim, methamidophos, chlorpyrifos, carbofuran, deltamethrin and atrazine when provided as the sole carbon and energy sources. Enzymatic analysis revealed that the MP degrading enzyme of strain Yw12 is an intracellular enzyme and is expressed constitutively. These results indicated that strain Yw12 might be used as a potential and effective organophosphate pesticides degrader for bioremediation of contaminated sites.

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Acknowledgments

We thank Shubo Gu (College of Agricultural Sciences, Shandong Agricultural University) for providing facilities and helping with GC analysis. We are most grateful to MARahman for critical reading of the manuscript. This study was supported by Hi-Tech Research and Development Program of China (No. 2008AA10Z402) and Basic Research Fund of CAAS (Nos. 0042009001, 0042011006).

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Authors and Affiliations

  1. Graduate School, Chinese Academy of Agricultural Sciences (GS of CAAS), 12 Zhongguancun Nandajie, Beijing, 100081, China

    Shenghui Wang & Yanchun Yan

  2. State Key Joint Laboratory of Environmental Simulation and Pollution Control, Department of Environmental Science and Engineering, Tsinghua University, Beijing, 100084, China

    Shenghui Wang

  3. College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100091, China

    Chen Zhang

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  1. Shenghui Wang
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  2. Chen Zhang
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Correspondence to Yanchun Yan.

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Wang, S., Zhang, C. & Yan, Y. Biodegradation of methyl parathion and p-nitrophenol by a newly isolated Agrobacterium sp. strain Yw12. Biodegradation 23, 107–116 (2012). https://doi.org/10.1007/s10532-011-9490-0

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