biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 50:767-770, 2006 | DOI: 10.1007/s10535-006-0127-9

Shoot and root culture of Hypericum perforatum L. transformed with Agrobacterium rhizogenes A4M70GUS

B. Vinterhalter1,*, S. Ninković1, A. Cingel1, D. Vinterhalter1
1 Institute for Biological Research "S. Stanković", Belgrade, Serbia and Montenegro

Hairy root cultures of Hypericum perforatum were obtained following inoculation of aseptically germinated seedlings with A. rhizogenes strain A4M70GUS. Effect of sucrose on the growth and biomass production of hairy root cultures was investigated. Hairy root cultures spontaneously regenerated shoots buds from which a number of shoot culture clones was established. Transformed shoot cultures exhibited good shoot multiplication, elongation and rooting on a hormone-free woody plant medium. Plants regenerated from hairy roots were similar in appearance to the normal, nontransformed plants.

Keywords: GUS activity; hairy roots; shoot regeneration; St. John's wort; sucrose
Subjects: Agrobacterium rhizogenes; cytokinins; β-glucuronidase; growth analysis, plant development, biomass and yield enhancement; hairy roots; Hypericum perforatum; in vitro culture, regeneration, proliferation, differentiation; in vitro culture, root cultivation, rooting; medicinal plants; nutrient medium, Murashige and Skoog; nutrient medium, Woody plant medium (WPM); polymerase chain reaction (PCR); root, rooting; St. John's wort; sucrose; sugars

Received: February 11, 2005; Accepted: June 17, 2005; Published: December 1, 2006  Show citation

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Vinterhalter, B., Ninković, S., Cingel, A., & Vinterhalter, D. (2006). Shoot and root culture of Hypericum perforatum L. transformed with Agrobacterium rhizogenes A4M70GUS. Biologia plantarum, 50(4), 767-770. doi: 10.1007/s10535-006-0127-9
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