biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:161-164, 2007 | DOI: 10.1007/s10535-007-0031-y

Withanolide production by in vitro cultures of Withania somnifera and its association with differentiation

M. Sharada1, A. Ahuja1,*, K. A. Suri1, S. P. Vij2, R. K. Khajuria1, V. Verma1, A. Kumar1
1 Regional Research Laboratory, CSIR, Jammu-Tawi, India
2 Shoolini Institute of Life Sciences & Business Management, Anand Campus, Solan, India

Withanolides-steroidal lactones, isolated from various Solanaceous plants have received considerable attention due to their potential biological activities. Five selected withanolides (withanone, withaferin A, withanolide A, withanolide B, withanolide E) were identified by HPLC-UV (DAD) - positive ion electrospray ionization mass spectroscopy in Withania somnifera (L.) Dunal cv. WSR plants and tissues cultured in vitro at different developmental phases. Cultures were established from five explants on Murashige and Skoog's medium supplemented with different plant growth regulators. Results suggest that production of withanolides is closely associated with morphological differentiation.

Keywords: callus culture; plant tissue culture; Solanaceae; steroidal lactones
Subjects: auxins; cytokinins; in vitro culture, callus growth and duration; in vitro culture, regeneration, proliferation, differentiation; nutrient medium, Gamborg (B5); nutrient medium, Murashige and Skoog (MS); Withania somnifera; withanolide

Received: May 4, 2005; Accepted: November 22, 2005; Published: March 1, 2007  Show citation

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Sharada, M., Ahuja, A., Suri, K.A., Vij, S.P., Khajuria, R.K., Verma, V., & Kumar, A. (2007). Withanolide production by in vitro cultures of Withania somnifera and its association with differentiation. Biologia plantarum, 51(1), 161-164. doi: 10.1007/s10535-007-0031-y
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