biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:641-646, 2007 | DOI: 10.1007/s10535-007-0136-3

Somatic embryogenesis and plant regeneration in Catharanthus roseus

A. Junaid1, A. Mujib1,*, M. A. Bhat1, M. P. Sharma1, J. Šamaj2
1 Cellular Differentiation and Molecular Genetics Section, Department of Botany, Faculty of Science, Hamdard University, New Delhi, India
2 Institute of Cellular and Molecular Botany, University of Bonn, Bonn, Germany

Embryogenic callus in Catharanthus roseus was initiated from hypocotyl on Murashige and Skoog's (MS) medium supplemented with 1.0-2.0 mg dm-3 of 2,4-dichlorophenoxyacetic acid (2,4-D) or chlorophenoxyacetic acid (CPA). Calli from other sources were non-embryogenic. Numerous somatic embryos were induced from primary callus on MS medium suplemented with naphthalene acetic acid (NAA) within two weeks of culture. Embryo proliferation was much faster on medium supplemented with 6-benzylaminopurine (BAP). After transfer to medium with gibberellic acid (GA3, 1.0 mg dm- 3) mature green embryos were developed and germinated well into plantlets on MS liquid medium supplemented with 0.5 mg dm-3 BAP. Later, embryos with cotyledonary leaves were subjected to different auxins treatments for the development of roots. Before transfer ex vitro, plantlets were cultivated on half strength MS medium containing 3 % sucrose and 0.5 mg dm-3 BAP for additional 2 weeks. Additionally, the effect of liquid medium has been evaluated at different morphogenetic stages.

Keywords: auxins; cytokinins; gibberellic acid; in vitro culture
Subjects: auxins; Cataranthus roseus; cytokinins; gibberellic acid, gibberellin; in vitro culture, embryogenesis, somatic embryogenesis; nutrient medium, Murashige and Skoog (MS); somatic embryogenesis

Received: November 29, 2005; Accepted: August 8, 2006; Published: December 1, 2007  Show citation

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Junaid, A., Mujib, A., Bhat, M.A., Sharma, M.P., & Šamaj, J. (2007). Somatic embryogenesis and plant regeneration in Catharanthus roseus. Biologia plantarum, 51(4), 641-646. doi: 10.1007/s10535-007-0136-3
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