biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 61:797-800, 2017 | DOI: 10.1007/s10535-017-0704-0

Ethanolamine induced modification in glycine betaine and proline metabolism in Nicotiana rustica under salt stress

S. Rajaeian1, A. A. Ehsanpour1,*, M. Javadi1, B. Shojaee1
1 Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran

The present study aimed to investigate the effects of ethanolamine on glycine betaine and proline metabolism in Nicotiana rustica under salt stress. The in vitro grown tobacco (Nicotiana rustica) plants were pretreated with ethanolamine (at concentrations 70, 130, 270, and 530 μM for biochemical analysis and only at the concentration of 530 μM for molecular analysis) and then transferred to Murashige and Skoog medium containing 200 mM NaCl for 3 weeks. Our results showed that ethanolamine promoted glycine betaine biosynthesis by an increase in betaine aldehyde dehydrogenase (BADH) gene expression and BADH enzymatic activity. Moreover, ethanolamine pretreatment possibly reduced proline content in salt stressed plants via its negative effect on Δ-pyrroline-5-carboxylate synthase (P5CS) gene expression and P5CS enzymatic activity and its positive effect on proline dehydrogenase (PDH) gene expression and PDH activity.

Keywords: betaine aldehyde dehydrogenase; Δ-pyrroline-5-carboxylate synthase; proline dehydrogenase
Subjects: ethanolamine; glycine betaine; proline; salinity; betaine aldehyde dehydrogenase; pyrroline-5-carboxylate synthetase; proline dehydrogenase; gene expression; tobacco

Received: June 14, 2016; Revised: November 19, 2016; Accepted: December 8, 2016; Published: December 1, 2017  Show citation

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Rajaeian, S., Ehsanpour, A.A., Javadi, M., & Shojaee, B. (2017). Ethanolamine induced modification in glycine betaine and proline metabolism in Nicotiana rustica under salt stress. Biologia plantarum, 61(4), 797-800. doi: 10.1007/s10535-017-0704-0
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