Skip to main content
Log in

Design and development of a microarray processing station (MPS) for automated miniaturized immunoassays

  • Published:
Biomedical Microdevices Aims and scope Submit manuscript

Abstract

Here we describe the design and evaluation of a fluidic device for the automatic processing of microarrays, called microarray processing station or MPS. The microarray processing station once installed on a commercial microarrayer allows automating the washing, and drying steps, which are often performed manually. The substrate where the assay occurs remains on place during the microarray printing, incubation and processing steps, therefore the addressing of nL volumes of the distinct immunoassay reagents such as capture and detection antibodies and samples can be performed on the same coordinate of the substrate with a perfect alignment without requiring any additional mechanical or optical re-alignment methods. This allows the performance of independent immunoassays in a single microarray spot.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • P. Angenendt, J. Glökler, Z. Konthur, H. Lehrach, D. J. Cahill, Anal. Chem. 75, 4368 (2003)

    Article  Google Scholar 

  • M. Cretich, F. Damin, M. Chiari, Analyst 139, 528 (2014)

    Article  Google Scholar 

  • R. M. Gonzalez, S. M. Varnum, and R. C. Zangar, Methods Pharmacol. Toxicol. Biomark. Methods Drug Discov. Dev. 273 (2008).

  • H. Li, S. Bergeron, M. G. Annis, P. M. Siegel, D. Juncker, Mol. Cell. Proteomics 14, 1024 (2015)

    Article  Google Scholar 

  • A. M. Lutz, J. K. Willmann, F. V. Cochran, P. Ray, S. S. Gambhir, PLoS Med. 5, e170 (2008)

    Article  Google Scholar 

  • S. Miersch, X. Bian, G. Wallstrom, S. Sibani, T. Logvinenko, C. H. Wasserfall, D. Schatz, M. Atkinson, J. Qiu, J. LaBaer, J. Proteome Res. 94, 486 (2013)

    Article  Google Scholar 

  • M. Pla-Roca, R. F. Leulmi, S. Tourekhanova, S. Bergeron, V. Laforte, E. Moreau, S. J. C. Gosline, N. Bertos, M. Hallett, M. Park, D. Juncker, Mol. Cell. Proteomics 11, M111.011460 (2012)

    Article  Google Scholar 

  • N. Rifai, M. A. Gillette, S. A. Carr, Nat. Biotechnol. 24, 971 (2006)

    Article  Google Scholar 

  • X. Yu, N. Schneiderhan-Marra, T. O. Joos, Clin. Chem. 56, 376 (2010)

    Article  Google Scholar 

  • G. Zhou, S. Bergeron, D. Juncker, J. Proteome Res. 14, 1872 (2015)

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain. The Nanobioengineering group at the Institute for Bioengineering of Catalonia (IBEC) receives support from the Commission for Universities and Research of the Department of Innovation, Universities and Enterprise of the Generalitat de Catalunya (No. 2014 SGR 1442). In addition, the research received support from the Spanish Ministry of Economy and Competitiveness, OLIGOCODES (MAT2012-38573-C02), and the Fundación Botín, Santander, Spain. M. Pla-Roca acknowledges a Beatriu de Pinos fellowship (2010 BP-B00180) from the Agència de Gestió d’Ajuts Universitaris i de Recerca (AGAUR).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Mateu Pla-Roca.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.s

Human and animal rights

“This article does not contain any studies with human participants or animals performed by any of the authors.”

Additional information

Mateu Pla-Roca and Gizem Altay contributed equally to this work.

Electronic supplementary material

ESM 1

Online Resource 1 (DOCX 6.75 mb)

ESM 2

Online Resource 2 (WMV 47.5 mb)

ESM 3

Online Resource 3 (WMV 27.5 mb)

ESM 4

Online Resource 4 (WMV 20.5 mb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Pla-Roca, M., Altay, G., Giralt, X. et al. Design and development of a microarray processing station (MPS) for automated miniaturized immunoassays. Biomed Microdevices 18, 64 (2016). https://doi.org/10.1007/s10544-016-0087-6

Download citation

  • Published:

  • DOI: https://doi.org/10.1007/s10544-016-0087-6

Keywords

Navigation