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Electrospun poly(ethylene oxide)/chitosan nanofibers with cellulose nanocrystals as support for cell culture of 3T3 fibroblasts

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Abstract

Chitosan/poly(ethylene oxide) (PEO) (5:1) nanofibers with cellulose nanocrystals (CNCs) were produced using an electrospinning technique. The addition of CNCs to the chitosan/PEO solutions allowed the production of uniform fibers (without beads) with a high proportion of chitosan. The fiber diameters were influenced by the concentration of CNCs in the chitosan/PEO solutions. The solutions containing 10% (w/w) of CNCs produced thinner fibers compared to solutions containing 5% (w/w) of CNCs. Thermogravimetric analysis indicated that the nanofibers were thermally stable, despite the CNCs having an effect on the PEO decomposition. Results from the cell assay in cultures of 3T3 fibroblasts indicated that the chitosan/PEO nanofibers (with 10% CNCs) promoted cell attachment with changes in the cytoskeletal organization. The results obtained in this work highlight the favorable effect of CNCs in electrospinning of chitosan/PEO. As expected, the influence of nanofibers on 3T3 fibroblasts F-actin and β-tubulin network revealed alterations in cytoskeleton, leading to changes in cell morphology and spreading.

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Acknowledgments

Support from Conselho Nacional de Desenvolvimento Científico (CNPq), Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and INCT Functional Complex Materials INOMAT (MCT/CNPq) are acknowledged. We thank Prof. Marcos Akira d’Àvila and Rosemeire dos Santos Almeida (Faculty of Mechanical Engineering—UNICAMP) for allowing us to use the electrospinning equipment, Douglas Soares by the atomic force microscopy analysis, Prof. Edvaldo Sabadini (Institute of Chemistry - UNICAMP) for allowing us to do the rheological tests and Thiago Heiji Ito by the viscosity measures.

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Authors and Affiliations

  1. Institute of Chemistry, Universidade Estadual de Campinas, CP 6154, CEP 13083-970, Campinas, SP, Brazil

    Daniela M. Ridolfi & Nelson Durán

  2. Institute of Science and Technology, Universidade Federal de São Paulo, São José dos Campos, SP, Brazil

    Ana Paula Lemes & Sheila de Oliveira

  3. Department of Biochemistry (Campus São Paulo), Universidade Federal de São Paulo, São Paulo, SP, Brazil

    Giselle Z. Justo & Marcelly V. Palladino

  4. Department of Biological Sciences (Campus Diadema), Universidade Federal de São Paulo, São Paulo, SP, Brazil

    Giselle Z. Justo

  5. NANOBIOSS (MCTI), Institute of Chemistry, Universidade Estadual de Campinas, Campinas, SP, Brazil

    Nelson Durán

  6. Brazilian National Nanotechnology Laboratory (LNNano-CNPEM), Campinas, SP, Brazil

    Nelson Durán

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  1. Daniela M. Ridolfi
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  2. Ana Paula Lemes
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  3. Sheila de Oliveira
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  4. Giselle Z. Justo
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  5. Marcelly V. Palladino
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  6. Nelson Durán
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Correspondence to Daniela M. Ridolfi or Nelson Durán.

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Ridolfi, D.M., Lemes, A.P., de Oliveira, S. et al. Electrospun poly(ethylene oxide)/chitosan nanofibers with cellulose nanocrystals as support for cell culture of 3T3 fibroblasts. Cellulose 24, 3353–3365 (2017). https://doi.org/10.1007/s10570-017-1362-2

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