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The molecular and physiological impact of bisphenol A in Sesamia nonagrioides (Lepidoptera: Noctuidae)

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Abstract

In the present study we investigated the potential relative effects of bisphenol A (BPA) and RH-5992 (tebufenozide) on the development and metamorphosis of the corn stalk borer, Sesamia nonagrioides (Lepidoptera: Noctuidae). A number of morphological and molecular factors were examined in order to identify the toxic and the endocrine-relative action of these two chemicals. We observed that BPA, RH-5992 and the combination of BPA/RH-5992 caused a developmental delay by extending the transition period between larval and pupal instars. These chemicals also reduced adult emergence and caused molting malformations during development and metamorphosis. In the corn stalk borer, BPA exhibits ecdysteroid activities in a fashion similar to that of the ecdysone agonist RH-5992. These results suggest that exposure to environmentally relevant concentrations of BPA during the early stages of the corn borer’s life cycle can result in various disorders that may be a consequence of endocrine disruption. The molecular mechanism by which BPA interferes with the physiological processes was also investigated. A significant induction was observed in the expression levels of the ecdysone-induced genes SnEcR and SnUSP, after injection of BPA and RH-5992. Additionally, we found that BPA acts as a very weak agonist of ecdysteroids in Bombyx mori derived Bm5 cell lines. From these cellular and molecular assays, our results brought evidence that BPA, like RH-5992, interferes with the ecdysteroidal pathways of the lepidopteran insect species.

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Authors and Affiliations

  1. Department of Biotechnology, School of Food, Biotechnology and Development, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Greece

    Dimitris Kontogiannatos, Giannis Zakasis & Anna Kourti

  2. Insect Molecular Genetics and Biotechnology Group, Institute of Biosciences & Applications, National Centre for Scientific Research ‘‘Demokritos’’, Athens, Greece

    Luc Swevers

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  1. Dimitris Kontogiannatos
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  2. Luc Swevers
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  4. Anna Kourti
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Correspondence to Anna Kourti.

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Kontogiannatos, D., Swevers, L., Zakasis, G. et al. The molecular and physiological impact of bisphenol A in Sesamia nonagrioides (Lepidoptera: Noctuidae). Ecotoxicology 24, 356–367 (2015). https://doi.org/10.1007/s10646-014-1384-6

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