Skip to main content

Advertisement

Log in

Random amplified polymorphic DNA analysis of the moth orchids, Phalaenopsis (Epidendroideae: Orchidaceae)

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

The genetic distance and relationships of 149 accessions representing 46 species in the genus Phalaenopsis and four species in Paraphalaenopsis were studied using random amplified polymorphic DNA (RAPD) markers. The genus Paraphalaenopsis was used as an outgroup. A total of 20 random primers were screened and out of these, six random primers provided 123 polymorphic bands and zero monomorphic bands. Pairwise genetic distances between accessions were estimated according to Nei and Li (1979). Cluster analysis of data using the UPGMA algorithm placed the species in seven groups that are mostly congruent with those based on morphological characters erected by previous workers. As observed from the banding patterns, Ph. doweryensis, which is suspected to be a hybrid of Ph. gigantea and Ph. kunstleri or Ph. cochlearis, is not. RAPD markers can thus be successfully applied in this economically important group of orchids for the study of relationships and to distinguish taxa up to the specific level.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Batchelor, S.R., 1982. Phalaenopsis. Part 1. Am Orchid Soc Bull 51: 1267–1275.

    Google Scholar 

  • Batchelor, S.R., 1983. Phalaenopsis. Part 3. Am Orchid Soc Bull 52(2): 124–128.

    Google Scholar 

  • Been, C.G., A.S. Na, J.B. Kim & H.Y. Kim, 2002. Random amplified polymorphic DNA (RAPD) for genetic analysis of Phalaenopsis species. J Kor Hort Sci 43: 387–391.

    Google Scholar 

  • Benner, M.S., M.D. Braunstein & M.U. Weisberg, 1995. Detection of DNA polymorphism within the genus Cattleya (Orchidaceae). Plant Mol Biol Rep 13(2): 147–155.

    Google Scholar 

  • Bose T.K. & S.K. Bhattacharjee 1980. Orchids of India. Calcutta, Naya Prokash.

  • Casiva, P.V., B.O. Saidman, J.C. Vilardi & A.M. Cialdella, 2002. First comparative phenetic studies of Argentinean species of Acacia (Fabaceae), using morphometric, isozymal, and RAPD approaches. Am J Bot 89: 843–845.

    Google Scholar 

  • Christenson, E.A., 2001. Phalaenopsis a monograph. Oregon, Timber Press.

  • Comber, J.B., 1972. A habitat note on Paphiopedilum chamberlainianum subsp. liemiana and Phalaenopsis sumatrana. Orchid Digest 36: 25.

    Google Scholar 

  • Davis, R.S., 1949. Philippine Phalaenopsis. Am Orchid Soc Bull 18: 743–749.

    Google Scholar 

  • Devos, K.M. & M.D. Gale, 1992. The use of random amplified polymorphic DNA markers in wheat. Theor Appl Genet 84: 567–572.

    Google Scholar 

  • Dressler, R.L., 1993. Phylogeny and classification of the orchid family. Oregon, Dioscorides Press.

  • Edwards, K.J., 1998. Randomly amplified polymorphic DNAs (RAPDs). In: A. Karp, P.G. Issac & D.C. Ingram (Eds.), Molecular Tools for Screening Biodiversity – Plants and Animals, pp. 171–179. Chapman and Hall, London.

  • Fu, Y.M., W.H. Chen, W.T. Tsai, Y.S. Lin, M.S. Chyou & Y.H. Chen, 1997. Phylogenetic studies of taxonomy and evolution among wild species of Phalaenopsis by Random Amplified DNA markers. Report of the Taiwan Sugar Research Institute 157: 27–42.

    Google Scholar 

  • Fu, Y.B., G. Peterson, A. Diederichsen & K.W. Richards, 2002. RAPD analysis of genetic relationships of seven flax species in the genus Linum L. Genet Resour Crop Ev 49: 253–259.

    Google Scholar 

  • Lakshmi, M., M. Parani & A. Parida, 2002. Molecular phylogeny of Mangroves IX. Molecular marker assisted intra-specific variation and species relationships in the Indian mangrove tribe Rhizophoreae. Aquat Bot 74: 201–217.

    Google Scholar 

  • Li, A., Y.B. Luo, Z.T. Xiong & G.E. Song, 2002. A preliminary study on conservation genetics of three endangered orchid species. Acta Bot Sin 44: 250–252.

    Google Scholar 

  • Lim, S.H., P.C.P. Teng, Y.H. Lee & C.J. Goh, 1999. RAPD analysis of some species in the genus Vanda (Orchidaceae). Ann Bot (London) 83: 193–196.

    Google Scholar 

  • MacPherson, J.M., P.E. Eckstein, G.J. Scoles & A.A. Gajadhar, 1993. Variability of the random amplified polymorphic assay among thermal cyclers and effects of primer and DNA concentration. Mol Cell Probe 7: 293–299.

    Google Scholar 

  • Muralidharan, K. & E.K. Wakeland, 1993. Concentration of primer and template qualitatively affects products in random amplified polymorphic DNA PCR. Biotechniques 14: 362–364.

    Google Scholar 

  • Nei, M. & W.H. Li, 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 74: 5269–5273.

    Google Scholar 

  • Pertwee, J., 1998. Phalaenopsis, the plant for 1999. Int Floricult Quart Rep 7: 7.

    Google Scholar 

  • Rajasegar, G., H.T.W. Tan, I.M. Turner & P.P. Kumar, 1997. Analysis of genetic diversity among Ixora cultivars (Rubiaceae) using Random Amplified Polymorphic DNA. Ann Bot (London) 80: 355–361.

    Google Scholar 

  • Rohlf, F.J., 2000. NTSYSpc – Numerical Taxonomy and Multivariate Analysis System (version 2.1). Exeter Software, Setauket, New York.

  • Rolfe, R.A., 1886. A Revision of the genus Phalaenopsis. The Gardeners’ Chronicle n.s., 26: 168–170, 212–213, 276–277.

  • Rushforth, K., R.P. Adams, M. Zhong, X. Ma & R.N. Pandey, 2003. Variation among Cupressus species from the easter hemisphere based on Random Amplified Polymorphic DNAs (RAPDs). Biochem Syst Ecol 31: 17–24.

    Google Scholar 

  • Schierwater, B. & A. Ender, 1993. Different thermostable DNA polymerases may amplify different RAPD products. Nucleic Acids Res 21: 4647–4648.

    Google Scholar 

  • Shim, P.S., 1982. A new generic classification in the Phalaenopsis-complex (Orchidaceae). Malayan Nat J 36: 1–28.

    Google Scholar 

  • Sudupak, M.A., M.S. Akkaya & A. Kence, 2002. Analysis of genetic relationships among perennial and annual Cicer species growing in Turkey using RAPD markers. Theor Appl Genet 105: 1220–1228.

    Google Scholar 

  • Sun, M. & K.C. Wong, 2001. Genetic structure of three orchid species with contrasting breeding systems using RAPD and allozyme markers. Am J Bot 88: 2180–2188.

    Google Scholar 

  • Sweet, H.R., 1980. The genus Phalaenopsis. California: The Orchid Digest Inc.

  • Teo, C.K.H., 1985. Native orchids of Peninsula Malaysia. Times Book International, Singapore.

  • Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Raflaski & S.V. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18: 6531–6535.

    CAS  PubMed  Google Scholar 

  • Wong, K.C. & M. Sun, 1999. Reproductive biology and conservation genetics of Goodyera procera (Orchidaceae). Am J Bot 86: 1406–1413.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to H. T. W. Tan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Goh, M.W.K., Kumar, P.P., Lim, S.H. et al. Random amplified polymorphic DNA analysis of the moth orchids, Phalaenopsis (Epidendroideae: Orchidaceae). Euphytica 141, 11–22 (2005). https://doi.org/10.1007/s10681-005-4814-y

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-005-4814-y

Key words

Navigation