Abstract
Over 150 resistance genes that confer resistance to either leaf rust, stripe rust or stem rust have been catalogued in wheat or introgressed into wheat from related species. A few of these genes from the ‘slow-rusting’ adult plant resistance (APR) class confer partial resistance in a race non-specific manner to one or multiple rust diseases. The recent cloning of two of these genes, Lr34/Yr18, a dual APR for leaf rust and stripe rust, and Yr36, a stripe rust APR gene, showed that they differ from other classes of plant resistance genes. Currently, seven Lr34/Yr18 haplotypes have been identified from sequencing the encoding ATP Binding Cassette transporter gene from diverse wheat germplasm of which one haplotype is commonly associated with the resistance phenotype. The paucity of well characterised APR genes, particularly for stem rust, calls for a focused effort in developing critical genetic stocks to delineate quantitative trait loci, construct specific BAC libraries for targeted APR genes to facilitate robust marker development for breeding applications, and the eventual cloning of the encoding genes.
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References
Agarwal S, Saini RG (2009) Undescribed wheat gene for partial leaf rust and stripe rust resistance from Thatcher derivatives RL6058 and 90RN2491 carrying Lr34. J Appl Genet 50:199–204
Bender CM, Pretorius ZA (2000) Expression of Lr34 resistance to wheat leaf rust in apical and basal flag leaf segments. Acta Phytopathol Entomol Hung 35:95–101
Borghi B (2001) Italian wheat pool. In: Bonjean AP, Angus WJ (eds) The world wheat book: a history of wheat breeding. Intercept, London, pp 289–309
Boyd LA, Smith PH, Hart N (2006) Mutants in wheat showing multipathogen resistance to biotrophic fungal pathogens. Plant Pathol 55:475–484
Caldwell RM (1968) Breeding for general and/or specific plant disease resistance. In: Finlay KW, Shepherd KW (eds) Proceedings of the 3rd international wheat genetics symposium. Australian Academy of Science, Canberra, pp 263–272
Cao S, Carver BF, Zhu X, Fang T, Chen Y, Hunger RM, Yan L (2010) A single-nucleotide polymorphism that accounts for allelic variation in the Lr34 gene and leaf rust reaction in hard winter wheat. Theor Appl Genet 121:385–392
Cloutier S, McCallum BD, Loutre C, Banks TW, Wicker T, Feuillet C, Keller B, Jordan MC (2007) Leaf rust resistance gene Lr1, isolated from bread wheat is a member of the large psr567 gene family. Plant Mol Biol 65:93–106
Dakouri A, McCallum BD, Walichnowski AZ, Cloutier S (2010) Fine-mapping of the leaf rust Lr34 locus in Triticum aestivum (L.) and characterization of large germplasm collections support the ABC transporter as essential for gene function. Theor Appl Genet 121:373–384
Dyck PL (1977) Genetics of leaf rust reaction in three introductions of common wheat. Can J Genet Cytol 19:711–716
Dyck PL (1987) The association of a gene for leaf rust resistance with the chromosome 7D suppressor of stem rust resistance in common wheat. Genome 29:467–469
Dyck PL, Kerber ER (1970) Inheritance in hexaploid wheat of adult-plant leaf rust resistance derived from Aegilops squarrosa. Can J Genet Cytol 12:175–180
Dyck PL, Kerber ER, Aung T (1994) An interchromosomal reciprocal translocation in wheat involving leaf rust resistance gene Lr34. Genome 37:556–559
Feuillet C, Travella S, Stein N, Albar L, Nublat A, Keller B (2003) Map-based isolation of the leaf rust disease resistance gene Lr10 from the hexaploid wheat (Triticum aestivum L.) genome. Proc Natl Acad Sci USA 100:15253–15258
Fu D, Uauy C, Distelfeld A, Blechl A, Epstein L, Chen X, Sela H, Fahima T, Dubcovsky J (2009) A kinase-START gene confers temperature-dependent resistance to wheat stripe rust. Science 323:1357–1360
Gill BS, Huang L, Kuraparthy V, Raupp WJ, Wilson DL, Friebe B (2008) Alien genetic resources for wheat leaf rust resistance, cytogenetic transfer, and molecular analysis. Aust J Agric Res 59:197–205
Hare RA (1997) Characterization and inheritance of adult plant stem rust resistance in durum wheat. Crop Sci 37:1094–1098
Hare RA, McIntosh RA (1979) Genetic and cytogenetic studies of durable adult-plant resistances in ‘Hope’ and related cultivars to wheat rusts. Z Pflanzenzüchtg 83:350–367
Herrera-Foessel SA, Singh RP, Huerta-Espino J, Crossa J, Djurlea A, Yuena J (2008) Genetic analysis of slow-rusting resistance to leaf rust in durum wheat. Crop Sci 48:2132–2140
Herrera-Foessel SA, Singh RP, Huerta-Espino J, Lagudah ES (2009) Characterization and mapping of a gene component for durable leaf rust resistance in chromosome arm 7BL. Phytopathology 99(Supplement):S53
Herrera-Foessel SA, Lagudah ES, Huerta-Espino J, Hayden M, Bariana HS, Singh D, Singh RP (2011) New slow rusting leaf rust and stripe rust resistance genes Lr67 and Yr46 in wheat are pleiotropic or closely linked. Theor Appl Genet 122:239–249
Hiebert CW, Thomas JB, Somers DJ, McCallum BD, Fox SL (2007) Microsatellite mapping of adult-plant leaf rust resistance gene Lr22a in wheat. Theor Appl Genet 115:877–884
Hiebert CW, Thomas JB, McCallum BD, Humphreys DG, DePauw RM, Hayden MJ, Mago R, Schnippenkoetter W, Spielmeyer W (2010). An introgression on wheat chromosome 4DL in RL6077 (Thatcher*6/PI 250413) confers adult plant resistance to stripe rust and leaf rust (Lr67). Theor Appl Genet 121:1083–1091
Huang L, Brooks SA, Li W, Fellers JP, Trick HN, Gill BS (2003) Map based cloning of leaf rust resistance gene Lr21 from the large and polyploidy genome of bread wheat. Genetics 164:655–664
Kerber ER, Aung T (1999) Leaf rust resistance gene Lr34 associated with nonsuppression of stem rust resistance in the wheat cultivar Canthatch. Phytopathology 89:518–521
Kolmer JA (1996) Genetics of resistance to wheat leaf rust. Annu Rev Phytopathol 34:435–455
Kolmer JA, Singh RP, Garvin DF, Viccars L, William HM, Huerta-Espino J, Ogbonnaya FC, Raman H, Orford S, Bariana HS, Lagudah ES (2008) Analysis of the Lr34/Yr18 rust resistance region in wheat germplasm. Crop Sci 48:1841–1852
Kota R, Spielmeyer W, McIntosh RA, Lagudah ES (2006) Fine genetic mapping fails to dissociate durable stem rust resistance gene Sr2 from pseudo black chaff in common wheat (Triticum aestivum L). Theor Appl Genet 112:492–499
Krattinger SG, Lagudah ES, Spielmeyer W, Singh RP, Huerta-Espino J, McFadden H, Bossolini E, Selter LL, Keller B (2009) A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323:1360–1363
Lagudah ES, McFadden HG, Singh RP, Huerta-Espino J, William M, Bariana H, Wellings C, Kong X, Dunn D, Appels RA (2007) Molecular genetic characterization of the multi-pathogenic adult plant disease resistance locus, Lr46/Yr29/Ltn2, in wheat. Isr J Plant Sci 55:333
Lagudah ES, Krattinger SG, Herrera-Foessel S, Singh RP, Huerta-Espino J, Spielmeyer W, Brown-Guedira G, Selter LL, Keller B (2009) Gene-specific markers for the wheat gene Lr34/Yr18/Pm38 which confers resistance to multiple fungal pathogens. Theor Appl Genet 119:889–898
Lillemo M, Asalf B, Singh RP, Huerta-Espino J, Chen XM, He ZH, Bjørnstad Å (2008) The adult plant rust resistance loci Lr34/Yr18 and Lr46/Yr29 are important determinants of partial resistance to powdery mildew in bread wheat line Saar. Theor Appl Genet 116:1155–1166
Loughman R, Lagudah ES, Shankar M, Wilson R, Jose K, Barclay I (2008) Wyalkatchem reselections differentiate the adult plant resistance gene Yr29 in an Australian wheat background. In: Appels R, Eastwood R, Lagudah E, Langridge P, Mackay M, McIntyre L, Sharp P (eds) Proceedings of the 11th international wheat genetics symposium. Sydney University Press, Sydney, p 133
Lukaszewski AJ (2000) Manipulation of the 1RS-1BL translocation in wheat by induced homoeologous recombination. Crop Sci 40:216–225
Mago R, Miah H, Lawrence GJ, Wellings CR, Spielmeyer W, Bariana HS, McIntosh RA, Pryor AJ, Ellis JG (2005) High resolution mapping and mutation analysis separate the rust resistance genes Sr31, Lr26 and Yr9 on the short arm of rye chromosome 1. Theor Appl Genet 112:41–50
Martinez F, Niks RE, Singh RP, Rubiales D (2001) Characterization of Lr46, a gene conferring partial resistance to wheat leaf rust. Hereditas 135:111–114
Mateos-Hernandez M, Singh RP, Hulbert SH, Bowden RL, Huerta-Espino J, Gill BS, Brown-Guedira G (2006) Targeted mapping of ESTs linked to the adult plant resistance gene Lr46 in wheat using synteny with rice 2. Funct Integr Genomics 6:122–131
McFadden ES (1930) A successful transfer of emmer characters to vulgare wheat. Agron J 22:1020–1034
McIntosh RA (1992) Close genetic linkage of genes conferring adult-plant resistance to leaf rust and stripe rust in wheat. Plant Pathol 41:523–527
McIntosh RA (2009) History and status of the wheat rusts. In: McIntosh R (ed) Proceedings of the 2009 BGRI technical workshop. Cd Sonora, Obregon, pp 11–24
McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts: an atlas of resistance genes. CSIRO, Melbourne
Njau PN, Jin Y, Huerta-Espino J, Keller B, Singh RP (2010) Identification and evaluation of sources of resistance to stem rust race Ug99 in wheat. Plant Dis 94:413–419
Pretorius ZA, Rijkenberg FHJ, Wilcoxson RD (1990) Influence of genetic background on the expression of wheat leaf rust resistance gene Lr22a. Phytopathology 80:579–584
Rosewarne GM, Singh RP, Huerta-Espino J, William HM, Bouchet S, Cloutier S, McFadden H, Lagudah ES (2006) Leaf tip necrosis, molecular markers and β1-proteasome subunits associated with the slow rusting resistance genes Lr46/Yr29. Theor Appl Genet 112:500–508
Rowland GG, Kerber ER (1974) Telocentric mapping in hexaploid wheat of genes for leaf rust resistance and other characters derived from Aegilops squarrosa. Can J Genet Cytol 16:137–144
Seah S, Spielmeyer W, Jahier J, Sivasithamparam K, Lagudah ES (2000) Families of resistance gene sequences within an introgressed chromosomal segment derived from T. ventricosum in wheat which confer resistance to nematode and rust pathogens. Mol Plant Microbe Interact 13:334–341
Singh RP (1992) Genetic association of leaf rust resistance gene Lr34 with adult plant resistance to stripe rust in bread wheat. Phytopathology 82:835–838
Singh RP, McIntosh RA (1984) Complementary genes for reaction to Puccinia recondita tritici in Triticum aestivum. 1. Genetic and linkage studies. Can J Genet Cytol 26:723–735
Singh RP, Mujeeb-Kazi A, Huerta-Espino J (1998) Lr46: a gene conferring slow-rusting to leaf rust in wheat. Phytopathology 88:890–894
Singh RP, Huerta-Espino J, Rajaram S (2000a) Achieving near-immunity to leaf and stripe rusts in wheat by combining slow rusting resistance genes. Acta Phytopathol Entomol Hung 35:133–139
Singh RP, Nelson JC, Sorrells ME (2000b) Mapping Yr28 and other genes for resistance to stripe rust in wheat. Crop Sci 40:1148–1155
Singh RP, Hodson DP, Huerta-Espino J, Jin Y, Njau P, Wanyera R, Herrera-Foessel SA, Ward RW (2008) Will stem rust destroy the world’s wheat crop? Adv Agron 98:271–309
Smith PH, Howie JA, Worland AJ, Stratford R, Boyd LA (2004) Mutations in wheat exhibiting growth-stage-specific resistance to biotrophic fungal pathogens. Mol Plant Microbe Interact 17:1242–1249
Spielmeyer W, McIntosh RA, Kolmer J, Lagudah ES (2005) Powdery mildew resistance and Lr34/Yr18 genes for durable resistance to leaf and stripe rust cosegregate at a locus on the short arm of chromosome 7D of wheat. Theor Appl Genet 111:731–735
Spielmeyer W, Singh RP, McFadden H, Wellings CR, Huerta-Espino J, Kong X, Appels R, Lagudah ES (2008) Fine scale genetic and physical mapping using interstitial deletion mutants of Lr34/Yr18: a disease resistance locus effective against multiple pathogens in wheat. Theor Appl Genet 116:481–490
Spielmeyer W, Mago R, Simkova H, Dolezel J, Krattinger S, Keller B, Paux E, Feuillet C, Breen J, Appels R, McIntosh R, Kota R, Wellings C, Lagudah E (2009) Durable rust resistance in wheat is effective against multiple pathogens. Plant and animal genomes XVII conference, San Diego. http://www.intl-pag.org/17/abstracts/W61_PAGXVII_425.html
Sunderwirth SD, Roelfs AP (1980) Greenhouse characterization of the adult plant resistance of Sr2 to wheat stem rust. Phytopathology 70:634–637
Uauy C, Brevis JC, Chen X, Khan I, Jackson L, Chicaiza O, Distelfeld A, Fahima T, Dubcovsky J (2005) High-temperature adult-plant (HTAP) stripe rust resistance gene Yr36 from Triticum turgidum ssp. dicoccoides is closely linked to the grain protein content locus Gpc-B1. Theor Appl Genet 112:97–105
William M, Singh RP, Huerta-Espino J, Ortiz Islas S, Hoisington D (2003) Molecular marker mapping of leaf rust resistance gene Lr46 and its association with stripe rust resistance gene Yr29 in wheat. Phytopathology 93:153–159
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Lagudah, E.S. Molecular genetics of race non-specific rust resistance in wheat. Euphytica 179, 81–91 (2011). https://doi.org/10.1007/s10681-010-0336-3
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DOI: https://doi.org/10.1007/s10681-010-0336-3