Abstract
The association between high malting quality and pre-harvest sprouting (PHS) susceptibility is a key challenge when developing new malting barley varieties. A new malting barley variety Baudin has successfully combined high malting quality and PHS tolerance. A doubled haploid population was developed for mapping PHS tolerance and seed dormancy from a cross of Baudin × AC Metcalfe using 233 molecular markers. Three QTLs were mapped for seed dormancy based on the standard germination test at 24, 48 and 72 h. One major QTL was mapped to the long arm of chromosome 5H controlling seed dormancy and PHS tolerance from Baudin. Two other minor QTLs were identified from Baudin on chromosomes 3 and 7H. QTL/QTL interaction was detected for seed dormancy between chromosomes 3 and 5H. The PHS tolerance allele of the 5H QTL from Baudin contributes to higher malt yield without significant impact on diastatic power, beta-glucan content and wort viscosity. QTL from Baudin provide new sources to integrate PHS tolerance and high malting quality.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barr AR, Karakousis A, Lance RCM, Logue SJ, Manning S, Chalmers KJ, Kretschmer JM, Collins HM, Roumeliotis S, Coventry S, Moody DB, Read BJ, Poulsen D, CD Li, Platz GJ, Inkerman A, Panozzo JF, Cullis BR, Smith AB, Lim P, Langridge P, Boyd WJR (2003) Mapping and QTL analysis of the barley population Chebec × Harrington. Aust J Agric Res 54:1125–1130
Bonnardeaux Y, Li C, Lance RCM, Zhang XQ, Sivasithamparam K, Appels R (2008) Seed dormancy in barley: identifying superior genotypes through incorporating epistatic interactions. Aust J Agric Res 59:517–526
Cakir M, Gupta S, Li C, Eckermann P, Hayden M, Ablett G, Platz G, Broughton S, Mather CD, Chalmers K, Loughman R, Appels R, Jones MGK, Galwey N, Lance R (2011) Genetic map construction and QTL analysis of the disease traits in the barley population Baudin × AC Metcalfe. Crop Pasture Sci 62:152–161
Carn JD (1980) Detection of rain damaged barley at harvest (pre-harvest sprouting). In: Proceedings of the thirtieth annual Australian cereal chemistry conference. Royal Australian Chemistry Institute, Melbourne, pp 34–38
Collins HM, Panozzo JF, Logue SJ, Jefferies SP, Barr AR (2003) Mapping and validation of chromosome regions associated with high malt extract in barley (Hordeum vulgare L.). Aust J Agric Res 54:1223–1240
Doerge RM, Churchill GA (1996) Permutation tests for multiple loci affecting a quantitative character. Genetics 142:285–294
European Brewery Convention (1987) Analytica, 4th edn. Brauerei und Getränke Rundschau, Zürich
Fox GP, Ferguson RA, Kelly AM, Inkerman PA (1991) Assessment of introduced barley varieties for pre-harvest sprouting resistance. Australian Cereal Chemistry Conference, pp 184–187
Fox G, Panozzo JF, Li CD, Lance RCM, Inkerman A, Henry RJ (2003) Molecular basis of barley quality. Aust J Agric Res 54:1081–1101
Gao W, Clancy JA, Han F, Prada D, Kleinhofs A, Ullrich SE (2003) Molecular dissection of a dormancy QTL region near the chromosome 7 (5H) L telomere in barley. Theor Appl Genet 107:552–559
Han F, Ullrich SE, Clancy JA, Jitkov V, Kilian A, Romagosa I (1996) Verification of barley seed dormancy loci via linked molecular markers. Theor Appl Genet 92:87–91
Li CD, Tarr A, Lance RCM, Harasymow S, Uhlmann J et al (2003) A major QTL controlling seed dormancy and pre-harvest sprouting/grain α-amylase in two-rowed barley (Hordeum vulgare L.). Aust J Agric Res 54:1303–1313
Li C, Ni P, Francki M, Hunter A, Zhang Y et al (2004) Genes controlling seed dormancy and pre-harvest sprouting in a rice–wheat–barley comparison. Funct Integr Genomic 4:84–93
Mares DJ (1983) Preservation of dormancy in freshly harvested wheat grain. Aust J Agric Res 34:33–38
Marquez-Cedillo LA, Hayes PM, Jones BL, Kleinhofs A, Legge WG, Rossnagel BG, Sato K, Ullrich SE, Wesenburg DM (2000) The North American barley genome mapping project. QTL analysis of malting quality in barley based on the doubled haploid progeny of two elite North American varieties representing different germplasm groups. Theor Appl Genet 103:625–637
Mather DE, Tinker NA, LaBerge DE, Edney M, Jones BL, Rossnagel BG, Legge WG, Briggs KG, Irvine RB, Falk DE, Kasha KJ (1997) Regions of the genome that affect grain and malt quality in a North American two-row barley cross. Crop Sci 37:544–554
Oberthur L, Dyer W, Ullrich SE, Blake TK (1995) Genetic analysis of seed dormancy in barley (Hordeum vulgare L.). J Quant Trait Loci. www.cabi-publishing.org/gateways/jag/index.html
Panozzo JF, Eckermann PJ, Mather DE, Moody DB, Black CK, Collins HM, Barr AR, Lim P, Cullis BR (2007) QTL analysis of malting quality traits in two barley populations. Aust J Agric Res 589:858–866
Prada D, Ullrich SE, Molina-Cano JL, Cistue L, Clancy JA, Romagosa I (2004) Genetic control of dormancy in a Triumph/Morex cross in barley. Theor Appl Genet 109:62–70
Romagosa I, Han F, Clancy JA, Ullrich SE (1999) Individual locus effects on dormancy during seed development and after-ripening in barley. Crop Sci 39:74–79
RVA Super3 Operation Manual (1998) Applications manual for the Rapid Visco Analyser
Takeda K (1996) Varietal variation and inheritance of seed dormancy in barley. In: Noda K, Mares D (eds) Pre-harvest sprouting in cereals 1995. Centre for Academic Societies, Osaka, pp 205–212
Thomas WTB, Powell W, Swanston JS, Ellis RP, Chalmers KJ, Barua UM, Jack P, Lea V, Forster BP, Waugh R, Smith DB (1996) Quantitative trait loci for germination and malting quality characters in spring barley cross. Crop Sci 36:265–273
Ullrich SE, Hayes PM, Dyer WE, Blake TK, Clancy JA (1993) Quantitative trait locus analysis of seed dormancy in steptoe barley. In: Walker-Simmons MK, Reid JL (eds) Pre-harvest in cereals 1992. American Society of Cereal Chemistry, St. Paul, pp 136–145
Ullrich SE, Han F, Gao W, Prada D, Clancy JA, Kleinhofs A, Romagosa I, Molina-Cano JL (2002) Summary of QTL analyses of the seed dormancy trait in barley. Barley Newsl 45:39–41. http://wheat.pw.sda.gov/ggpages/Barley
Ullrich SE, Clancy JA, del Blanco IA, Lee H, Jitkov VA, Han F, Kleinhofs A, Matsui K (2008) Genetic analysis of pre-harvest sprouting in six-row barley cross. Mol Breed 21:249–259
van Der Schaar W, Alonso-Blanco C, Leon-Kloosterziel KM, Jansen RC, van Ooijen JW, Koornneef M (1997) QTL analysis of seed dormancy in Arabidopsis using recombinant inbred lines and MQM mapping. Heredity 79:190–200
Von Korff M, Wang H, Leon J, Pillen K (2008) AB-QTL analysis in spring barley: III. Identification of exotic alleles for the improvement of malting quality in spring barley (H. vulgare ssp. spontaneum). Mol Breed 21:81–93
Yang J, Hu CC, Ye XZ, Zhu J (2005) QTLNetwork 2.0. Institute of Bioinformatics, Zhejiang University, Hangzhou. http://ibi.zju.edu.cn/software/qtlnetwork
Zhang F, Chen G, Huang Q, Orion O, Krugman T, Fahima T, Korol AB, Nevo E, Gutterman Y (2005) Genetic basis of barley caryopsis dormancy and seedling desiccation tolerance at the germination stage. Theor Appl Genet 110:445–453
Acknowledgments
The project is supported by the Australian Grain Research and Development Corporation, Australian Winter Cereal Molecular Marker Program and CRC Molecular Plant Breeding. We thank Ms Cathy Burchell for her support with the field experiments.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zhang, XQ., Westcott, S., Panozzo, J. et al. Comparative analysis of Australian and Canadian barleys for seed dormancy and malting quality. Euphytica 188, 103–111 (2012). https://doi.org/10.1007/s10681-011-0576-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10681-011-0576-x