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Hypericum perforatum L. Seed Germination: Interpopulation Variationand Effect of Light, Temperature, Presowing Treatments and Seed Desiccation

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Abstract

Germplasm conservation of medicinal plants is of increasing interest and, when possible, seed banking is the most efficient system for ex situ conservation of these plant genetic resources. Hypericum perforatum L. (St. John's wort, Guttiferae) is a medicinal plant with evidence of efficacy as an anti-depressant. The aim of this work was to increase knowledge of its seed germination behaviour by studying 68 wild populations. Seed germination tests were carried out at 25/15 °C under a photoperiod of 16-h light/8-h darkness. Final germination percentages were highly variable depending on the accession, ranging from 6 to 98%. Similarly, germination rate (as expressed by T 50 values) varied significantly from 6.1 to 23.0 days. The effect of seed desiccation with silica gel on subsequent germination was also studied. The effect of two other incubation temperatures (15 and 25 °C) and light (photoperiod or darkness) on seed germination was studied in several accessions. Temperature had no significant effect on final germination percentages. However, light significantly increased the germination of most but not all accessions assayed. Seeds from four accessions with low germination percentages were subjected to different presowing treatments that could increase germination: dry heat, hot water and gibberellic acid. Germination was promoted significantly by gibberellic acid in two of the four accessions assayed, but the thermal treatments did not enhance significantly the germination percentages. This study reveals that conclusions based on one population of Hypericum perforatum cannot characterize the germination behaviour for the entire species.

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Authors and Affiliations

  1. Departamento de Biología Vegetal, Escuela Universitaria de Ingeniería Técnica Agrícola, Universidad Politécnica de Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain

    F. Pérez-García, M. Huertas & M. E. González-Benito

  2. CIA de Albadalejito, Ctra. Cuenca-Tarancón km 7, 16194, Cuenca, Spain

    E. Mora & B. Peña

  3. CRF-INIA, Apdo. 1045 28000 Alcalá de Henares, Madrid, Spain

    F. Varela

Authors
  1. F. Pérez-García
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  2. M. Huertas
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  3. E. Mora
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  4. B. Peña
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  5. F. Varela
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  6. M. E. González-Benito
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Correspondence to M. E. González-Benito.

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Pérez-García, F., Huertas, M., Mora, E. et al. Hypericum perforatum L. Seed Germination: Interpopulation Variationand Effect of Light, Temperature, Presowing Treatments and Seed Desiccation. Genet Resour Crop Evol 53, 1187–1198 (2006). https://doi.org/10.1007/s10722-005-2012-3

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  • DOI: https://doi.org/10.1007/s10722-005-2012-3

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