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High yielding indigo sources in native Isatis (Brassicaceae) taxa from Turkey

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Abstract

Indigo dye has a distinctive blue color and is one of the oldest known dyestuffs. In order to identify new Isatis genotypes for indigo production, 14 Isatis taxa collected from native area were grown under field conditions in Kahramanmaraş, Turkey. The effects of taxa and harvest times on leaf, indigo and indican yield were analyzed and compared with culture species (Isatis tinctoria and Isatis indigotica) by using spectrophotometer and HPLC. High indigo yield (87.13 kg/ha) was obtained from Isatis constricta and I. tinctoria subsp. tomentolla (Mersin location) had high indican yield (12.25 mg/g) in fresh leaves. The present work identified high indigo yielding taxa that may be used for genetic improvement in order to re-introduce Isatis species in the agricultural systems of Mediterranean regions. Especially I. constricta can be evaluated in terms of higher indigo yield than the native and culture taxa. I. constricta is an endemic plant in endangered category. This situation reveals that the need for the conservation of the species. To the best of our knowledge, there is no previous study reported about indigo contents of the studied 11 Isatis spp. so far.

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References

  • Ali S, Hussain T, Nawaz R (2009) Optimization of alkaline extraction of natural dye from Henna leaves and its dyeing on cotton by exhaust method. J Clean Prod 17:61–66. doi:10.1016/j.jclepro.2008.03.002

    Article  CAS  Google Scholar 

  • Angelini LG, Campeol E, Tozzi S, Gilbert KG, Cooke DT, John P (2003) A new HPLC-ELSD method to quantify indican in Polygonum tinctorium L. and to evaluate β-glucosidase hydrolysis of indican for indigo production. Biotechnol Prog 19:1792–1797. doi:10.1021/bp0300218

  • Angelini LG, Tozzi S, Nassi N (2007) Differences in leaf yield and indigo precursors production in woad (Isatis tinctoria L.) and Chinese woad (Isatis indigotica Fort.) genotypes. Field Crops Research 101:285–295. doi:10.1016/j.fcr.2006.12.004

    Article  Google Scholar 

  • Biertümpfel A, Hammer M, Reinhold G, Vetter A (2013) Waid (Isatis tinctoria L.). In: Hoppe B (ed) Handbuch des Arznei- und Gewürzanbaus, vol 5. Saluplanta, Bernburg, pp 671–684

    Google Scholar 

  • Blackburn RS, Bechtold T, John P (2009) The development of indigo reduction methods and pre-reduced indigo products. Color Technol 125:193–207. doi:10.1111/j.1478-4408.2009.00197.x

    Article  CAS  Google Scholar 

  • Campeol E, Angelını L, Tozzı S, Bertolaccı M (2006) Seasonal variation of indigo precursors in Isatis tinctoria L. and Polygonum tinctorium Ait. as affected by water deficit. Environ Exp Bot 58(1–3):223–233. doi:10.1016/j.envexpbot.2005.09.006

  • Chen S, Zhu Z, Ma H, Yang J, Guo Q (2014) DNA barcodes for discriminating the medicinal plant Isatis indigotica Fort. (Cruciferae) and its adulterants. Biochem Syst Ecol 57:287–292. doi:10.1016/j.bse.2014.08.007

    Article  CAS  Google Scholar 

  • Çömlekçioğlu N (2011) Effects of different sowing times on the yield components of some Isatis species (woad) survived in Kahramanmaraş and determination of dyeing properties and dyestuff content. Kahramanmaraş Sütçü İmam University. Institue of Science, Department of Field Crops. Ph. D. Thesis. Kahramanmaraş

  • Davis PH (1965) Flora of Turkey and the east Aegean Islands, vol I. Edinburg at the University Press, Edinburg, pp 287–366

    Google Scholar 

  • Ekim T, Koyuncu M, Vural M, Duman H, Aytaç Z, Adıgüzel N (2000) Red data book of Turkish plants. Türkiye Tabiyatını Kurma Derneği, Ankara

    Google Scholar 

  • Gilbert KG, Maule HG, Rudolph B, Lewis M, Vandenburg H, Sales E, Tozzi S, Cooke DT (2004) Quantitative analysis of indigo and indigo precursors in leaves of Isatis spp. and Polygonum tinctorium. Biotechnol Prog 20:1289–1292. doi:10.1021/bp0300624

    Article  CAS  PubMed  Google Scholar 

  • Guarnaccia P, Pinio M, Testa G, Branca F (2012) Woad (Isatis tinctoria L.): an innovative crop for the mediterranean ago-industrial system. In: VI International symposium on brassicas and XVIII crucifer genetics workshop. Catania, Italy. http://www.brassica2012.it/

  • Guinot P, Roge A, Gargadennec A, Garcia M, Dupont D, Lecoeur E, Candelier L, Andary C (2006) Dyeing plants screening: an approach to combine past heritage and present development. Color Technol 122:93–101. doi:10.1111/j.1478-4408.2006.00015.x

    Article  CAS  Google Scholar 

  • Karaman S, Ilcim A, Celik M, Çömlekcioğlu N, Dıraz E (2014) Cultivation trials. Dying process and dyestuffs content of native Isatis species which are distrubuted in Kahramanmaras and near surrounding area. TUBITAK 109O655 Project Final Report. Kahramanmaraş

  • Kızıl S (2000) Investigations on determination of suitable sowing density and dyeing properties of some woad species (Isatis tinctoria L., Isatis constricta Davis). Ankara University Graduate School of Natural and Applied Sciences Department of Field Crops. Ph. D. Thesis Ankara

  • Kokubun T, Edmonds J, John P (1997) Indoxyl derivatives in woad in relation to medieval indigo production. Phytochemistry 49:79–87. doi:10.1016/S0031-9422(97)01069-8

    Article  Google Scholar 

  • Lıau BC, Jong TT, Lee MR, Chen SS (2007) LC-APCI-MS method for detection and analysis of tryptanthrin, indigo and indirubin in Daqingye and Banlangen. J Pharm Biomed Anal 43:346–351. doi:10.1016/j.jpba.2006.06.029

    Article  PubMed  Google Scholar 

  • Maugard T, Enaud E, Choisy P, Legoy MD (2001) Identification of an indigo precursor from leaves of Isatis tinctoria (Woad). Phytochemistry 58:897–904. doi:10.1016/S0031-9422(01)00335-1

    Article  CAS  PubMed  Google Scholar 

  • Minami Y, Nishimura O, Hara-Nishimura I, Nishimura M, Matsubara H (2000) Tissue and intracellular localization of indican and the purification and characterization of indican synthase from indigo plants. Plant Cell Physiol 41:218–225. doi:10.1093/pcp/41.2.218

    Article  CAS  PubMed  Google Scholar 

  • Oberthur C, Schneider B, Gaf H, Hamburger M (2004) The elusive indigo precursors in woad (I. tinctoria L.) identification of the major indigo precursor, Isatan A, and a structure revision of Isatan B. Chem Biodivers 1:174–182. doi:10.1002/cbdv.200490009

    Article  PubMed  Google Scholar 

  • Osimani A, Aquilanti L, Baldini G, Silvestri G, Butta A, Celementi F (2012) Implement of a biotechnological process for vat dyeing with woad. Ind Microbial Biotechnol 39:1139–1144. doi:10.1007/s10295-012-1139-4

    Google Scholar 

  • Rocha L, Carvalho C, Martins S, Braga F, Carnide V (2011) Morpho-agonomic Characterization and variation of indigo precursors in woad (Isatis tinctoria L.) accessions. Plant Genet Res: Charact Util 9:206–209. doi:10.1017/S1479262111000499

    Article  CAS  Google Scholar 

  • Sales E, Kanhonou R, Baixauli C, Giner A, Cooke D, Gilbert K, Arrilaga I, Segura J, Ros R (2006) Sowing date, transplanting, plant density and nitrogen fertilization affect indigo production from Isatis species in a mediterranean region of Spain. Ind Crops Prod 23:29–39. doi:10.1016/j.indcrop.2005.03.002

    Article  Google Scholar 

  • Samanta AK, Agarwal P (2009) Application of natural dyes on textiles. Indian J Fibre Textile 34:3

    Google Scholar 

  • Song J, Imanaka H, Imamura K, Kajitani K, Nakanishi K (2010) Development of a highly efficient indigo dyeing method using indican with an immobilized β-glucosidase from Aspergillus niger. J Biosci Bioeng 110:281–287. doi:10.1016/j.jbiosc.2010.03.010

    Article  CAS  PubMed  Google Scholar 

  • Stoker KG, Cooke DT, Hill DJ (1998) An improved method for the large-scale processing of woad (Isatis tinctoria) for possible commercial production of woad indigo. J Agric Eng Res 71:315–320. doi:10.1006/jaer.1998.0329

    Article  Google Scholar 

  • Tansı S (1998) The research on plant performance of woad (Isatis tinctoria L.) under the Çukurova conditions. J Field Crops Central Res Inst 7:38–43

    Google Scholar 

  • Tozzi S, Lercari B, Angelini LC (2005) Light quality influences indigo precursors production and seed germination in Isatis tinctoria L. and Isatis indigotica Fort. Photochem Photobiol 81:914–919. doi:10.1111/j.1751-1097.2005.tb01462

    Article  CAS  PubMed  Google Scholar 

  • Wu E, Komolpis K, Wang HY (1999) Chemical extraction of indigo from Indigofera tinctoria while attaining biological integrity. Biotechnol Tech 13:567–569

    Article  CAS  Google Scholar 

  • Yıldırımlı S (1988) The revision of the genus Isatis L. (Cruciferae) in Western half and North of Turkey. Doga Turk J Bot 12:332–400

    Google Scholar 

  • Zou P, Koh LH (2007) Determination of indican, isatin, indirubin and indigotin in Isatis indigotica by liquid chromatography/electrospray ionization tandem mass spectrometry. Rapid Commun Mass Spectrom 21:1239–1246. doi:10.1002/rcm.2954

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was funded by the TUBITAK. The Scientific and Technological Research Council of Turkey (TOVAG-109O655). The authors are thankful to Dr. Esin İspir, Chemistry Dept. for her valuable suggestions and comments about LC–MS results.

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Authors and Affiliations

  1. Biology Department, Faculty of Science and Letters, Kahramanmaraş Sütçü İmam University, Kahramanmaraş, 46100, Turkey

    Şengül Karaman, Emel Diraz, Nazan Çömlekçioğlu & Hasan Durdu

  2. Biology Department, Faculty of Science and Letters, Mustafa Kemal University, Antakya, Turkey

    Ahmet İlçim

  3. Field Crops Department, Agricultural Faculty, Cukurova University, Adana, Turkey

    Sezen Tansi

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  1. Şengül Karaman
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  2. Emel Diraz
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  3. Nazan Çömlekçioğlu
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  4. Ahmet İlçim
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  5. Hasan Durdu
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  6. Sezen Tansi
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Correspondence to Şengül Karaman.

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Karaman, Ş., Diraz, E., Çömlekçioğlu, N. et al. High yielding indigo sources in native Isatis (Brassicaceae) taxa from Turkey. Genet Resour Crop Evol 63, 531–543 (2016). https://doi.org/10.1007/s10722-015-0269-8

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  • DOI: https://doi.org/10.1007/s10722-015-0269-8

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