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Identification of endocrine cells of the stomach that express acid-sensitive background potassium (K2P9.1/TASK3) channels

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Abstract

The family of two-pore domain potassium (K2P) channels is important in setting and controlling the background potassium current of excitable cells. This study examines the localisation of the acid-sensitive channel, K2P9.1 (TASK3), in cells of the gastric mucosa. We observed K2P9.1 immunoreactivity in endocrine cells of the mucosal glands of the guinea-pig, rat and mouse but the channels were not detected in parietal, chief, or mucous cells. K2P9.1 channel immunoreactivity was consistently co-localised with histidine decarboxylase immunopositive enterochromaffin-like (ECL) cells, and with the majority of ghrelin immunoreactive X/A cells. Localisation in somatostatin immunoreactive D cells was rare in the guinea-pig, and did not occur in the stomach of rat, but, in the mouse, K2P9.1 channels were observed in the majority of somatostatin-immunoreactive D cells. Conversely, sections taken from the guinea-pig and mouse stomachs, but not rat stomach, revealed K2P9.1 in gastrin-containing G cells. These results demonstrate the presence of K2P9.1 channels in the entero-endocrine ECL, G and D cell populations of the stomach that regulate acid secretion through the release of histamine, gastrin and somatostatin. K2P9.1 channels were located in the ghrelin X/A cells that regulate food intake.

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Acknowledgments

This work was supported by the National Health and Medical Research Council of Australia (grant 566696) and Novartis Pharmaceuticals AG. The authors wish to thank Dr B.A. Nayagam (University of Melbourne) for valuable feedback on the manuscript.

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Authors and Affiliations

  1. Department of Anatomy & Cell Biology, University of Melbourne, Parkville, VIC, 3010, Australia

    Karina Needham, Louise Pontell, Billie Hunne, Michelle Thacker & John B. Furness

  2. Novartis Horsham Research Centre, Horsham, West Sussex, RH12 5AB, UK

    Damian McHugh

Authors
  1. Karina Needham
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  2. Louise Pontell
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  3. Billie Hunne
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  4. Michelle Thacker
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  5. Damian McHugh
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  6. John B. Furness
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Correspondence to Karina Needham.

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Needham, K., Pontell, L., Hunne, B. et al. Identification of endocrine cells of the stomach that express acid-sensitive background potassium (K2P9.1/TASK3) channels. J Mol Hist 41, 403–409 (2010). https://doi.org/10.1007/s10735-010-9276-4

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  • DOI: https://doi.org/10.1007/s10735-010-9276-4

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