Abstract
To investigate the spatial and temporal immunolocalisation and staining intensity of the Notch signalling family in endometrium of fertile and infertile women, endometrial biopsies were collected by curettage from 25 fertile women across the menstrual cycle and 10 infertile women in the mid secretory phase of menstrual cycle. Immunohisotchemistry was completed for NOTCH1, -2, -3, -4, cleaved Notch, DLL1, -3, -4, JAGGED1, -2, HES and NUMB and immunostaining intensity measured in both the endometrial glandular and luminal epithelium. NOTCH1 and the ligands DLL1 and JAGGED1 were key proteins displaying increased staining intensity during the receptive phase of the menstrual cycle and dysregulated in infertile endometrium. Conversely, NUMB a negative regulator of Notch signalling was decreased in the mid secretory phase of the menstrual cycle in fertile women and increased with infertility.
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This work was supported by NHMRC (Australia) Project Grant (#1027100) and by the Victorian Government’s Operational Infrastructure Support Program. ED was supported by NHMRC (Australia) Senior Research Fellowship (#550905).
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Michelle Van Sinderen and Carly Cuman are the co-first authors.
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Van Sinderen, M., Cuman, C., Gamage, T. et al. Localisation of the Notch family in the human endometrium of fertile and infertile women. J Mol Hist 45, 697–706 (2014). https://doi.org/10.1007/s10735-014-9587-y
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DOI: https://doi.org/10.1007/s10735-014-9587-y