Abstract
The neuronal apoptosis inhibitory protein (NAIP) is a constituent of the NLRC4 inflammasome, which plays a key role in innate immunity, and an antiapoptotic protein. Recently, we reported the previously undescribed role of NAIP in cell division. The liver is one of the body’s most actively regenerative organs. Given the novel mitotic role of NAIP, we examined its expression in hepatic mass restoration. The major liver lobe of Wistar rats was removed, and samples from both newly formed liver tissue, assessed by positive Ki67 immunostaining, and the remnant, intact liver lobes from hepatectomized rats were taken 3 and 7 days after surgery. Naip5 and Naip6 mRNA levels were significantly higher in regenerating hepatic tissue than in intact liver lobe tissue, and this increase was also observed at the protein level. Naip5 and Naip6 mRNA in situ hybridization showed that this increase occurred in the hepatic parenchyma. The histology of the regenerated liver tissue was normal, with the exception of a noticeable deficiency of hepatic lobule central veins. The results of this study suggest the involvement of NAIP in liver mass restoration following partial hepatectomy.
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The datasets generated during and/or analyzed during the current study are available from the corresponding author upon reasonable request.
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Acknowledgements
Julio Plaza-Díaz is part of the “UGR Plan Propio de Investigación 2016” and the “Excellence actions: Unit of Excellence on Exercise and Health (UCEES), University of Granada”. The authors would like to thank the University of Granada CIC microscopy personnel for their help.
Funding
This work was supported by the “Visiting Scholars P22-2017 Program, Plan Propio de Investigación de la Universidad de Granada” (to FAM), and by Grant #PI-0538-2017 from Junta de Andalucía, Spain (to LF).
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FAM and LF contributed to the study concept and design. LF, VMC, and MJSL did the hepatectomies. JPD, AIAM, CRS, STC, VMC, MNO and MJSL participated in the acquisition of data. JPD, AIAM, MNO, and CRS did the statistical analysis. All authors took part in the analysis and interpretation of data, the drafting of the manuscript and the critical revision of the manuscript. JPD, AIAM, AM, LFG and FAM wrote the manuscript.
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The procedures observed the guidelines for animal research of the University of Granada (Spain) and the Ethics Committee for Animal Experimentation of Junta de Andalucía, Spain (reference 06-04-15-321).
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Plaza-Díaz, J., Álvarez-Mercado, A.I., Robles-Sánchez, C. et al. NAIP expression increases in a rat model of liver mass restoration. J Mol Histol 52, 113–123 (2021). https://doi.org/10.1007/s10735-020-09928-y
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DOI: https://doi.org/10.1007/s10735-020-09928-y