Skip to main content
Log in

Live birth following transfer of a cryopreserved embryo generated from a cryopreserved oocyte and a cryopreserved sperm: Case report

  • Case Report
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

As is the case with non-frozen oocytes, the efficient and successful use of cryopreserved oocytes in human assisted reproduction is, in part, dependent on the ability to apply selection criteria when choosing the ‘best’ embryos for transfer from a cohort. In many cases this, in turn, will necessitate the cryopreservation of non-transferred embryos to minimise the risk of multiple pregnancy. It is therefore important to establish that an embryo, generated by fertilization of a frozen-thawed oocyte, can be capable of surviving subsequent cryopreservation while retaining the potential for normal development. In this case report, we document the delivery of a normal male infant following transfer of a frozen-thawed embryo, generated by the fertilization of a frozen-thawed oocyte by a frozen-thawed sperm.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Gook DA, Osborn SM, Johnston WI. Cryopreservation of mouse and human oocytes using 1,2-propanediol and the configuration of the meiotic spindle. Hum Reprod 1993;8:1101–9

    PubMed  CAS  Google Scholar 

  2. Gook DA, Osborn SM, Bourne H, Johnston WI. Fertilization of human oocytes following cryopreservation; normal karyotypes and absence of stray chromosomes. Hum Reprod 1994;9:684–91

    PubMed  CAS  Google Scholar 

  3. Gook DA, Osborn SM, Johnston WI. Parthenogenetic activation of human oocytes following cryopreservation using 1,2-propanediol. Hum Reprod 1995;10:654–8

    PubMed  CAS  Google Scholar 

  4. Gook DA, Schiewe MC, Osborn SM, Asch RH, Jansen RP, Johnston WI. Intracytoplasmic sperm injection and embryo development of human oocytes cryopreserved using 1,2-propanediol. Hum Reprod 1995;10:2637–41

    PubMed  CAS  Google Scholar 

  5. Porcu E, Fabbri R, Damiano G, Giunchi S, Fratto R, Ciotti PM, Venturoli S, Flamigni C. Clinical experience and applications of oocyte cryopreservation. Mol Cell Endocrinol 2000;169:33–7

    Article  PubMed  CAS  Google Scholar 

  6. Borini A, Bonu MA, Coticchio G, Bianchi V, Cattoli M, Flamigni C. Pregnancies and births after oocyte cryopreservation. Fertil Steril 2004;82:601–5

    Article  PubMed  Google Scholar 

  7. Chen SU, Lien YR, Chen HF, Chang LJ, Tsai YY, Yang YS. Observational clinical follow-up of oocyte cryopreservation using a slow-freezing method with 1,2-propanediol plus sucrose followed by ICSI. Hum Reprod 2005;20:1975–80

    Article  PubMed  CAS  Google Scholar 

  8. Boldt J, Tidswell N, Sayers A, Kilani R, Cline D. Human oocyte cryopreservation: 5-year experience with a sodium-depleted slow freezing method. Reprod Biomed Online 2006;13:96–100

    Article  PubMed  CAS  Google Scholar 

  9. Borini A, Sciajno R, Bianchi V, Sereni E, Flamigni C, Coticchio G. Clinical outcome of oocyte cryopreservation after slow cooling with a protocol utilizing a high sucrose concentration. Hum Reprod 2006;21:512–7

    Article  PubMed  CAS  Google Scholar 

  10. Levi Setti PE, Albani E, Novara PV, Cesana A, Morreale G. Cryopreservation of supernumerary oocytes in IVF/ICSI cycles. Hum Reprod 2006;21:370–5

    Article  PubMed  CAS  Google Scholar 

  11. Bourne H, Richings N, Harari O, Watkins W, Speirs AL, Johnston WI, Baker HW. The use of intracytoplasmic sperm injection for the treatment of severe and extreme male infertility. Reprod Fertil Dev 1995;7:237–45

    Article  PubMed  CAS  Google Scholar 

  12. Edgar DH, Bourne H, Speirs AL, McBain JC. A quantitative analysis of the impact of cryopreservation on the implantation potential of human early cleavage stage embryos. Hum Reprod 2000;15:175–9

    Article  PubMed  CAS  Google Scholar 

  13. Ziebe S, Petersen K, Lindenberg S, Andersen AG, Gabrielsen A, Andersen AN. Embryo morphology or cleavage stage: how to select the best embryos for transfer after in-vitro fertilization. Hum Reprod 1997;12:1545–9

    Article  PubMed  CAS  Google Scholar 

  14. Shoukir Y, Campana A, Farley T, Sakkas D. Early cleavage of in-vitro fertilized human embryos to the 2-cell stage: a novel indicator of embryo quality and viability. Hum Reprod 1997;12:1531–6

    Article  PubMed  CAS  Google Scholar 

  15. Sakkas D, Percival G, D’Arcy Y, Sharif K, Afnan M. Assessment of early cleaving in vitro fertilized human embryos at the 2-cell stage before transfer improves embryo selection. Fertil Steril 2001;76:1150–6

    Article  PubMed  CAS  Google Scholar 

  16. Lundin K, Bergh C, Hardarson T. Early embryo cleavage is a strong indicator of embryo quality in human IVF. Hum Reprod 2001;16:2652–7

    Article  PubMed  CAS  Google Scholar 

  17. Revel A, Moshe N, Helman A, Safran A, Simon A, Koler M. Mouse embryos generated from frozen-thawed oocytes can successfully survive a second cryopreservation. Hum Reprod 2004;19:666–9

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to David H. Edgar.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gook, D.A., Hale, L. & Edgar, D.H. Live birth following transfer of a cryopreserved embryo generated from a cryopreserved oocyte and a cryopreserved sperm: Case report. J Assist Reprod Genet 24, 43–45 (2007). https://doi.org/10.1007/s10815-006-9093-6

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10815-006-9093-6

Keywords

Navigation