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Genotoxicity of dental resin polymerization initiators in vitro

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Abstract

The polymerization initiators for resins cured using visible light usually consist of a photosensitizer, primarily camphorquinone (CQ), and a reducing agent, which is often a tertiary amine (DMPT, DMAEMA), while the initiator used for self-curing resins consists of benzoyl peroxide (BPO) and a tertiary amine (DMPT). The genotoxicities of camphorquinone (CQ), benzoyl peroxide (BPO), dimethyl-para-toluidine (DMPT), 2-dimethylamino-ethyl-methacrylate (DMAEMA), and 1-allyl-2-thiourea (ATU) were examined using the bioluminescent bacterial genotoxicity test. 4-Nitroquinoline-N-oxide (4NQO) was prepared for comparison with these chemicals. Acetone solutions of the five polymerization initiators and 4NQO were prepared.

Benzoyl peroxide (BPO), dimethyl-para-toluidine (DMPT), and 1-allyl-2-thiourea (ATU) showed significant genotoxic activity at 24 h in the bioluminescent bacterial genotoxicity test, at concentrations of approximately 5 μM, 4 mM, and 1 mM, respectively. 2-Dimethyloamino-ethyl-methacrylate (DMAEMA) did not have genotoxic activity and CQ had questionable genotoxic activity. In comparison, 4NQO had strong genotoxicity, at 4 μM, roughly the same as that of BPO. Therefore, BPO should be used carefully in clinical dentistry.

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Authors and Affiliations

  1. Department of Biomaterials Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan

    Y. Nomura & M. Okazaki

  2. Special Dental Clinic Section, Dental Hospital, Hiroshima University, Hiroshima, Japan

    W. Teshima

  3. Department of Operative Dentistry, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan

    T. Kawahara & N. Tanaka

  4. Graduate School of Science and Technology, Nagasaki University, Nagasaki, Japan

    H. Ishibashi

  5. Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, Kumamoto, Japan

    K. Arizono

Authors
  1. Y. Nomura
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  2. W. Teshima
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  3. T. Kawahara
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  4. N. Tanaka
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  5. H. Ishibashi
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  6. M. Okazaki
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  7. K. Arizono
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Correspondence to Y. Nomura.

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Nomura, Y., Teshima, W., Kawahara, T. et al. Genotoxicity of dental resin polymerization initiators in vitro. J Mater Sci: Mater Med 17, 29–32 (2006). https://doi.org/10.1007/s10856-006-6326-2

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  • DOI: https://doi.org/10.1007/s10856-006-6326-2

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