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Inflammatory response on injection of chitosan/GP to the brain

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Abstract

Chitosan is a well-known biomaterial that, with the addition of glycerophosphate salt (GP), gels at physiological temperatures and therefore is useful for tissue engineering purposes. This study examines the procedure of injecting chitosan/ GP to the brain in order to form a gel track. The gel system and surgical technique were successful in this endeavour; however, on examining the inflammatory response to the material it was found that the chitosan/GP was wholly engulfed by macrophages within 7 days. This was determined by staining for both the gel and the macrophages, an important technique for localising injected material. The chitosan/GP-containing macrophages formed a neat tract at the lesion site, but after 45 days no chitosan/GP was found. It was concluded that, although chitosan/GP is present after implantation, it is not available for direct scaffolding in the brain.

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Authors and Affiliations

  1. Department of Materials Engineering, Monash University, Wellington Road, Clayton, 3168, Australia

    K. E. Crompton & J. S. Forsythe

  2. CRC for Polymers, 32 Business Park Drive, Notting Hill, VIC, 3168, Australia

    K. E. Crompton & J. S. Forsythe

  3. Howard Florey Institute of Experimental Physiology and Medicine, The University of Melbourne, Parkville, 3010, Australia

    D. Tomas, D. I. Finkelstein, M. Marr & M. K. Horne

Authors
  1. K. E. Crompton
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  2. D. Tomas
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  3. D. I. Finkelstein
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  4. M. Marr
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  5. J. S. Forsythe
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  6. M. K. Horne
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Correspondence to M. K. Horne.

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Crompton, K.E., Tomas, D., Finkelstein, D.I. et al. Inflammatory response on injection of chitosan/GP to the brain. J Mater Sci: Mater Med 17, 633–639 (2006). https://doi.org/10.1007/s10856-006-9226-6

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  • DOI: https://doi.org/10.1007/s10856-006-9226-6

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