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Kinetic characterization and comparison of various protein crosslinking reagents for matrix modification

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Abstract

We have characterized the relative efficacies of a number of protein crosslinking agents that have the potential for use in the crosslinking of proteinaceous matrices both in vitro and in vivo. The crosslinkers tested were; l-threose (LT), Genipin (GP), Methylglyoxal (MG), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), proanthrocyanidin (PA) and glutaraldehyde (GA). The relative effectiveness of the crosslinkers with regard to their saturating concentrations was: GA > PA > EDC > MG = GP ≫ LT. Most of the crosslinkers displayed a pH dependence and were more effective at more alkaline pH. At optimal pH and saturating conditions, the relative reaction rates of the crosslinkers were: PA = GA > EDC > GP > MG ≫ LT.

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Acknowledgment

This work was supported by the National Institutes of Health (1R43 AR055014-01).

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  1. Orthopeutics, L.P., 111 Cooperative Way, Suite 210, Georgetown, TX, 78626, USA

    Paul Slusarewicz, Keng Zhu & Tom Hedman

  2. Department of Biomedical Engineering, Texas A&M University, College Station, TX, USA

    Tom Hedman

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  1. Paul Slusarewicz
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Correspondence to Paul Slusarewicz.

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Slusarewicz, P., Zhu, K. & Hedman, T. Kinetic characterization and comparison of various protein crosslinking reagents for matrix modification. J Mater Sci: Mater Med 21, 1175–1181 (2010). https://doi.org/10.1007/s10856-010-3986-8

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  • DOI: https://doi.org/10.1007/s10856-010-3986-8

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