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Dynamics of lysozyme and its hydration water under an electric field

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Abstract

The effects of a static electric field on the dynamics of lysozyme and its hydration water are investigated by means of incoherent quasi-elastic neutron scattering (QENS). Measurements were performed on lysozyme samples, hydrated respectively with heavy water (D 2O) to capture the protein dynamics and with light water (H 2O), to probe the dynamics of the hydration shell, in the temperature range from 210 < T < 260 K. The hydration fraction in both cases was about ∼ 0.38 gram of water per gram of dry protein. The field strengths investigated were respectively 0 kV/mm and 2 kV/mm ( ∼2 × 10 6 V/m) for the protein hydrated with D 2O and 0 kV and 1 kV/mm for the H 2O-hydrated counterpart. While the overall internal protons dynamics of the protein appears to be unaffected by the application of an electric field up to 2 kV/mm, likely due to the stronger intra-molecular interactions, there is also no appreciable quantitative enhancement of the diffusive dynamics of the hydration water, as would be anticipated based on our recent observations in water confined in silica pores under field values of 2.5 kV/mm. This may be due to the difference in surface interactions between water and the two adsorption hosts (silica and protein), or to the existence of a critical threshold field value E c ∼2–3 kV/mm for increased molecular diffusion, for which electrical breakdown is a limitation for our sample.

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Acknowledgments

We acknowledge the use of the DAVE software in part of the data analysis [32]. We thank C. Stanley at ORNL for stimulating discussions. It is also a pleasure to acknowledge R. Goyette, R. Mills, D. Maierhafer, R. Moody and M. Loguillo at SNS for valuable technical support. PF acknowledges the GEM fellowship program at UTK. HON and QZ acknowledge the support of the Center for Structural Molecular Biology at ORNL supported by the U.S. DOE, Office of Science, Office of Biological and Environmental Research Project ERKP291. Work at ORNL and SNS is sponsored by the Scientific User Facilities Division, Office of Basic Energy Sciences, U.S. DOE.

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Authors and Affiliations

  1. Quantum Condensed Matter Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA

    P. M. Favi & S. O. Diallo

  2. Department of Materials Science and Engineering, University of Tennessee, Knoxville, TN, 37996, USA

    P. M. Favi

  3. Biology and Soft Matter Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA

    Q. Zhang & H. O’Neill

  4. Chemical and Engineering Materials Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA

    E. Mamontov

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  1. P. M. Favi
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  2. Q. Zhang
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  3. H. O’Neill
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  4. E. Mamontov
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  5. S. O. Diallo
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Correspondence to S. O. Diallo.

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Favi, P.M., Zhang, Q., O’Neill, H. et al. Dynamics of lysozyme and its hydration water under an electric field. J Biol Phys 40, 167–178 (2014). https://doi.org/10.1007/s10867-014-9343-2

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  • DOI: https://doi.org/10.1007/s10867-014-9343-2

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