Abstract
The purpose of this study was to characterize the heterogeneity of oxygen partial pressure in different adipose tissue zones and to assess the possibility of compensating these heterogeneities during optical glucose measurements. In this proof of concept study, the heterogeneity of oxygen partial pressure was determined in the adipose tissue of a pig by using 48 oxygen sensors in 3 zones of the abdominal region at two different blood oxygen levels. Sensor oxygen values correlated well with reference blood oxygen values and we identified heterogeneities in oxygen partial pressure among the defined zones of the abdominal region. Significant differences in the mean oxygen partial pressure were found when comparing the three abdominal zones but no significant differences were found when comparing two sensors located in close proximity (on one cannula). The low heterogeneity on one cannula allows the compensation of physiological oxygen variations for optical glucose measurements by using an additional oxygen sensor in close proximity to the glucose sensor. In addition, this setup can be used to continuously monitor tissue oxygenation e.g. in patients with adipose tissue dysfunction or serve limb ischemia.
This is a preview of subscription content, log in via an institution to check access.
References
Hodson L. Adipose tissue oxygenation. Adipocyte. 2014;3:75–80.
Rumpler M, Mader JK, Fischer JP, et al. First application of a transcutaneous optical single-port glucose monitoring device in patients with type 1 diabetes mellitus. Biosens Bioelectron. 2017;88:240–8.
Rumpler M, Hajnsek M, Klimant I, et al () Compensation of oxygen partial pressure variations during optical, enzymatic glucose sensing in adipose tissue. Diabetes Technol Ther. 2015;17:A89.
Borisov SM, Nuss G, Klimant I. Red light-excitable oxygen sensing materials based on platinum (II) and palladium (II) benzoporphyrins. Anal Chem. 2008;80:9435–42.
Montero-Baker MF, Au-Yeung KY, Wisniewski NA, et al. The First-in-Man “si Se Puede” Study for the use of micro-oxygen sensors (MOXYs) to determine dynamic relative oxygen indices in the feet of patients with limb-threatening ischemia during endovascular therapy. J Vasc Surg. 2015;61(1501–1510):e1.
Acknowledgements
This work was funded by the European Union Framework 7 Program during the project SPIDIMAN (reference No 305343). The authors wish to thank the Biomedical Research Unit at the Medical University of Graz for the support during the experiments as well as Selma Mautner for her assistance in preparing this manuscript.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Ethical approval
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the Biomedical Research Unit at the Medical University of Graz. All animal experiments were approved by the Austrian federal government and were performed in consent with Directive 2010/63/EU on the protection of animals used for scientific purposes.
Research involving human and animal rights
This article does not contain any studies with human participants performed by any of the authors.
Rights and permissions
About this article
Cite this article
Rumpler, M., Hajnsek, M., Baumann, P. et al. Monitoring tissue oxygen heterogeneities and their influence on optical glucose measurements in an animal model. J Clin Monit Comput 32, 583–586 (2018). https://doi.org/10.1007/s10877-017-0034-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10877-017-0034-y