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First Derivative Synchronous Fluorescence Spectroscopy for the Simultaneous Determination of Sulpiride and Mebeverine Hydrochloride in Their Combined Tablets and Application to Real Human Plasma

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Abstract

A rapid, simple and highly sensitive first derivative synchronous fluorometric method has been developed for the simultaneous analysis of binary mixture of sulpiride (SUL) and mebeverine hydrochloride (MEB). The method is based upon measurement of the synchronous fluorescence intensity of these drugs at ∆λ = 100 nm in water. The different experimental parameters affecting the fluorescence of the two drugs were carefully studied and optimized. The fluorescence-concentration plots were rectilinear over the range of 0.05–1 µg/mL and 0.2–3.2 µg/mL for SUL and MEB respectively with lower detection limits (LOD) of 0.006 and 0.01 µg/mL and quantification limits (LOQ) of 0.0.02 and 0.05 µg/mL for SUL and MEB, respectively. The proposed method was successfully applied for the determination of the two compounds in synthetic mixtures and in commercial tablets. The high sensitivity attained by the proposed method allowed the determination of both of SUL and MEB metabolite (veratic acid) in real human plasma samples applying second derivative synchronous fluorometric technique. The mean% recoveries (n = 3) for both MEB metabolite (veratic acid) and SUL were 99.82 ± 2.53 and 98.84 ± 6.20 for spiked human plasma respectively, while for real human plasma, the mean% recoveries (n = 3) were 91.49 ± 4.25 and 91.36 ± 8.46 respectively.

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References

  1. Sweetman SC (2007) Martindale: the complete drug reference, 35th edn. The Pharmaceutical Press, London, pp 119–692

    Google Scholar 

  2. The British Pharmacopoeia, The Stationary Office, London, 2007 (electronic version)

  3. El-Didamony AM (2008) Spectrochim Acta 69:770–775

    Article  Google Scholar 

  4. Shama SA, Amin AS (2004) Spectrochim Acta 60:1769–1774

    Article  CAS  Google Scholar 

  5. Sreedhar K, Sastry CSP, Reddy MN, Sankar DG (1997) Mikrochim Acta 131–135

  6. Reddy MN, Rao KVS, Sankar DG, Sridhar K (1996) Indian Drugs 33:604–606

    CAS  Google Scholar 

  7. Hassan EM, Gazy AA, Bedair MM (1995) Drug Dev Ind Pharm 21:633–638

    Article  CAS  Google Scholar 

  8. Abdel-Gawad FM (1994) Egypt J Anal Chem 3:129–134

    CAS  Google Scholar 

  9. Bedair MM, Korany MA, Ebdel-Hay MA, Gazy AA (1990) Analyst (London) 115:449–453

    Article  CAS  Google Scholar 

  10. Ibrahim H, Issa YM, Abu-Shawish HM (2007) J Pharm Biomed Anal 44:8–15

    Article  CAS  PubMed  Google Scholar 

  11. Ibrahim H, Issa YM, Abu-Shawish HM (2005) J Pharm Biomed Anal 36:1053–1061

    Article  CAS  PubMed  Google Scholar 

  12. Perrin C, Vander-Heyden Y, Maftouh M, Massart DL (2001) Electrophoresis 22:3203–3215

    Article  CAS  PubMed  Google Scholar 

  13. De-Schutter JA, Van-Der-Weken G, Van-den-Bossche W, De-Moerloose P (1985) J Chromatogr 20:739–742

    Article  CAS  Google Scholar 

  14. De-Schutter JA, Van-Der-Weken G, Van-den-Bossche W, De-Moerloose P (1985) J Chromatogr 350:135–144

    Article  CAS  Google Scholar 

  15. Radwan MA, Abdine HH, Aboul-Enein HY (2006) Biomed Chromatogr 20:211–216

    Article  CAS  PubMed  Google Scholar 

  16. Elliott S, Burgess V (2006) J Anal Toxicol 30:91–97

    CAS  PubMed  Google Scholar 

  17. Arayne MS, Sultana N, Siddiqui FA (2005) J Pharm Sci 18:11–14

    CAS  Google Scholar 

  18. Al-Deep O, Al-Hadiya BM, Foda NH (1997) Chromatographia 44:427–430

    Article  Google Scholar 

  19. Al-Angary AA, Khadr SH, Abd-Elhady SS, Bayomi MA, Mahrous GM (1992) Anal Lett:1251–1260

  20. Hoogewijs G, Massart DL (1986) J Chromatogr Biomed Appl 50:391–398

    Google Scholar 

  21. De-Schutter JA, De-Croo F, Van-der-Weken G, Van-den-Bossche W, De-Moerloose P (1985) Chromatographia 20:185–192

    Article  CAS  Google Scholar 

  22. Zheng T, Li SF, Xu BZ (1999) Yaowu-Fenxi-Zazhi 19:10–12

    CAS  Google Scholar 

  23. Buna M, Aaron JJ, Prognon P, Mahuzier G (1996) Analyst (Cambridge,UK) 121:1551–1556

    CAS  Google Scholar 

  24. Nie JF, Wu HL, Xia AL, Zhu SH, Bian YC, Li SF, Yu RO (2007) Anal Sci 23:1377–1382

    Article  CAS  PubMed  Google Scholar 

  25. Farghaly OAEM (2000) J Pharm Biomed Anal 23:783–791

    Article  CAS  PubMed  Google Scholar 

  26. Zeng YH, Song Q (1997) Fenxi-Huaxue 25:56–58

    Google Scholar 

  27. Yamamoto E, Sakaguchi T, Kajima T, Mano N, Asakawa NJ (2004) Chromatogr B 807:327–334

    Article  CAS  Google Scholar 

  28. Wang JZ, Xu X, Hu JG, Li J (2004) Fenxi-Ceshi-Xuebao 23:121–122

    CAS  Google Scholar 

  29. Matthijs N, Perrin C, Maftouh M, Massrat DL, Vander-Heyden Y (2004) J Chromatogr A 1041:119–133

    Article  CAS  PubMed  Google Scholar 

  30. Kagan M, Chlenov M, Kraml CM (2004) J Chromatogr A 1033:321–331

    Article  CAS  PubMed  Google Scholar 

  31. Liu SL, Liu CH, Qin YJ, Zhao QQ, Liu XX, Li FL (2002) Yaowu-Fenxi-Zazhi 22:365–368

    Google Scholar 

  32. Chiba R, Ogasawara A, Kubo T, Yamazaki Y, Umino M, Ishizuka Y (2003) Anal Sci 19:785–790

    Article  CAS  PubMed  Google Scholar 

  33. Huang MC, Ho HO, Yeh GC, Ke WT, Lin LC, Hsu TMB, Sheu MT (2002) Yaowu-Shipin-Fenxi 10:1–6

    CAS  Google Scholar 

  34. Huang MC, Ho HO, Yeh GC, Ke WT, Lin LC, Hsu TMB, Kao CC, Sheu MT (2001) J Chromatogr B 763:157–163

    Article  CAS  Google Scholar 

  35. Zhang ZL, Zhou JL (2007) J Chromatogr A 1154:205–213

    Article  CAS  PubMed  Google Scholar 

  36. Hassib ST, Moussa BA, Hashim HA, El-Zaher AA (2002) Spectrosc Lett 35:43–61

    Article  CAS  Google Scholar 

  37. El-Walily AFM, El-Gingy A, Bedair MF (1999) J Pharm Biomed Anal 21:535–548

    Article  CAS  PubMed  Google Scholar 

  38. Zayed SIM (2005) Anal Sci 21:985–998

    Article  CAS  PubMed  Google Scholar 

  39. Chen GZ, Huang XZ, Xu JG, Zheng ZZ, Wang ZB (1990) The methods of fluorescence analysis, 2nd edn. Science, Beijing, 112

    Google Scholar 

  40. Patra D, Mishra AK (2002) Anal Chem 21:787

    CAS  Google Scholar 

  41. Lloyd JBF (1971) Nat Phys Sci 231:64, http://www.chemistrymag.org/eji/2003/058065pe.htm. 2003; vol. 5 No. 8:65

    CAS  Google Scholar 

  42. Murillo Pulgarin JA, Alanon Molina A, Fernandez Lopez P (1998) Anal Chim Acta 370:9–18

    Article  CAS  Google Scholar 

  43. Walash MI, Belal F, El-Enany N, Abdelal AA (2008) J Fluoresc 18:61–74

    Article  CAS  PubMed  Google Scholar 

  44. El-Enany N (2008) J AOAC INT 91:542–550

    CAS  PubMed  Google Scholar 

  45. Wei YF, Li XH, Ma DM (2005) Guang Pu Xue Yu Guang Pu Fen Xi 25:588–590

    CAS  PubMed  Google Scholar 

  46. Murillo Pulgarín JA, Alañón Molina A, Fernández López P, Sánchez-Ferrer Robles I (2007) Anal Chim Acta 583:55–62

    Article  PubMed  Google Scholar 

  47. Xiao Y, Wang HY, Han J (2005) Acta A Mol Biomol Spectrosc 61:567–573

    Article  Google Scholar 

  48. Abdelal A, El-Enany N, Belal F (2009) Talanta 80:880–888

    Article  CAS  PubMed  Google Scholar 

  49. De-Schutter J, De-Croo F, Van-der-weken G, Van-den-Bossche W, De-Moerloose P (1985) Chromatographia 20:185–192

    Article  CAS  Google Scholar 

  50. The United States Pharmacopoeia USP30 (2007) (electronic version)

  51. ICH Harmonized Tripartite Guideline, Validation of analytical procedures: Text and methodology, Q2(R1), Current Step 4 Version, Parent Guid lines ON Methodology Dated November 6, 1996, Incorporated in November 2005. http://www.Ich.org/LOB/media/MEDIA417.pdf (accessed February 15,2008).

  52. Miller JC, Miller JN (2005) Statistics and chemometrics for analytical chemistry, 5th edn. Printce Hall, England, 256

    Google Scholar 

  53. Ronald G, Peter V, Mechael E, Wayne D (1991) J Pharm Sci 80:952–957

    Article  Google Scholar 

  54. Stockis A, Guelen PJM, de Vos D (2001) J Pharm Biomed Anal 29:335–340

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, 35516, Mansoura, Egypt

    M. Walash, M. Sharaf El-Din, Nahed El-Enany, M. Eid & Sh. Shalan

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  1. M. Walash
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  2. M. Sharaf El-Din
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  3. Nahed El-Enany
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  4. M. Eid
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  5. Sh. Shalan
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Correspondence to M. Eid.

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Walash, M., Sharaf El-Din, M., El-Enany, N. et al. First Derivative Synchronous Fluorescence Spectroscopy for the Simultaneous Determination of Sulpiride and Mebeverine Hydrochloride in Their Combined Tablets and Application to Real Human Plasma. J Fluoresc 20, 1275–1285 (2010). https://doi.org/10.1007/s10895-010-0679-0

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