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Spectrofluorimetric Method for Determination and Validation of Cefixime in Pharmaceutical Preparations Through Derivatization with 2-Cyanoacetamide

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Abstract

A simple, sensitive and accurate method has been developed for spectrofluorimetric determination of cefixime in pure form and pharmaceutical preparations. The method is based on the reaction of cefixime with 2-cyanoacetamide in the presence of 21% ammonia at 100 °C. The fluorescent reaction product showed maximum fluorescence intensity at λ 378 nm after excitation at λ 330 nm. The factors affecting the derivatization reaction were carefully studied and optimized. The fluorescence intensity versus concentration plot was rectilinear over the range of 0.02 to 4 μg mL−1 with correlation coefficient of 0.99036. The limit of detection (LOD) and limit of quantification (LOQ) was found to be 2.95 ng mL−1 and 9.84 ng mL−1, respectively. The proposed method was validated statistically and through recovery studies. The method was successfully applied for the determination of cefixime in pure and dosage form with percent recoveries from 98.117% to 100.38%. The results obtained from the proposed method have been compared with the official HPLC method and good agreement was found between them.

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Authors and Affiliations

  1. Institute of Chemical Sciences, University of Peshawar, Peshawar, Pakistan

    Jasmin Shah, M. Rasul Jan, Sultan Shah &  Inayatullah

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  1. Jasmin Shah
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  2. M. Rasul Jan
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  3. Sultan Shah
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  4. Inayatullah
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Correspondence to Jasmin Shah.

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Shah, J., Jan, M.R., Shah, S. et al. Spectrofluorimetric Method for Determination and Validation of Cefixime in Pharmaceutical Preparations Through Derivatization with 2-Cyanoacetamide. J Fluoresc 21, 579–585 (2011). https://doi.org/10.1007/s10895-010-0745-7

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  • DOI: https://doi.org/10.1007/s10895-010-0745-7

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