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Photoactivated Green Fluorescence Emission by Femtosecond Oscillator from Indole Solutions

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Abstract

We reported a novel femtosecond-laser-activated fluorescence emission from indole solutions upon excitation by the second harmonic wavelength of a femtosecond oscillator. A new absorption band around 400 nm and corresponding fluorescent band in the green domain were produced after the irradiation of femtosecond laser. This femtosecond-laser-activated luminescence process that allows the use of visible wavelength as a substitute for UV light to excite fluorescence from indole would extend applications based on indole chromophore. Furthermore, the photoactived emission can act as a fluorescence lifetime probe to measure the polarity in complex biological systems since it is polarity-sensitive. High performance liquid chromatography with fluorescence detector (HPLC-FLU) and high performance liquid chromatography with mass spectrometer (HPLC-MS) analysis demonstrate that the origin of the photoactivated fluorescence is new molecular species that generated in indole solution upon femtosecond laser irradiation.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (No.10804116). The authors would like to thank the professor Joseph R. Lakowicz at the University of Maryland School Of Medicine for his constructive suggestions and Professor Ya Cheng at SIOM for his stimulating discussion.

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Authors and Affiliations

  1. State Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, P. O. Box 800–211, Shanghai, 201800, China

    Chen Wang, Li Liu, Lingling Qiao, Jielei Ni & Jian Xu

  2. Instrumental Analysis Center, Shanghai Jiao Tong University, Shanghai, 200240, China

    Lei Feng & Yuanlong Wang

  3. No. 390 Qinghe Rd., Jiading, Shanghai, China, 201800

    Chen Wang

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  1. Chen Wang
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  2. Lei Feng
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  3. Li Liu
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  4. Yuanlong Wang
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  5. Lingling Qiao
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  6. Jielei Ni
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  7. Jian Xu
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Correspondence to Chen Wang.

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Wang, C., Feng, L., Liu, L. et al. Photoactivated Green Fluorescence Emission by Femtosecond Oscillator from Indole Solutions. J Fluoresc 21, 2185–2191 (2011). https://doi.org/10.1007/s10895-011-0921-4

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  • DOI: https://doi.org/10.1007/s10895-011-0921-4

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