Abstract
A highly sensitive fluorescent probe for clenbuterol hydrochloride (CLB) detection has been first designed based on its catalytic effect on NaIO4 oxidating eosine Y (R). And this environment-friendly, simple, rapid, selective and sensitive fluorescent probe has been utilized to detect CLB in the practical samples with the results consisting with those obtained by GC/MS. The structures of R and CLB were characterized by infrared spectra. The mechanism of the proposed assay for the detection of CLB was also discussed.
Similar content being viewed by others
References
He P, Shen L, Liu R, Luo Z, Li Z (2011) Direct detection of β-agonists by use of gold nanoparticle-based colorimetric assays. Anal Chem 83:6988–6995
Nath N, Chilkoti A (2004) Label free colorimetric biosensing using nanoparticles. J Fluoresc 14:377–389
Parr MK, Opfermann G, Schänzer W (2009) Analytical methods for the detection of clenbuterol. Bioanalysis 1:437–450
Sharma D, Sahoo SK, Bera RK, Kamal R (2013) Spectroscopic and computational study of a naphthalene derivative as colorimetric and fluorescent sensor for bioactive anions. J Fluoresc 23:387–392
Gaichore RR, Srivastava AK (2012) Multiwalled carbon nanotube-4-tert-butyl calixarene composite electrochemical sensor for clenbuterol hydrochloride determination by means of differential pulse adsorptive stripping voltammetry. J Appl Electrochem 42:979–987
Wang H, Zhang Y, Li H, Du B, Ma H, Wu D, Wei Q (2013) A silver-palladium alloy nanoparticle-based electrochemical biosensor for simultaneous detection of ractopamine, clenbuterol and salbutamol. Biosens Bioelectron 49:14–19
Evans RC, Douglas P, Williams JG, Rochester DL (2006) A novel luminescence-based colorimetric oxygen sensor with a “traffic light” response. J Fluoresc 16:201–206
Melwanki MB, Huang SD, Fuh MR (2007) Three-phase solvent bar microextraction and determination of trace amounts of clenbuterol in human urine by liquid chromatography and electrospray tandem mass spectrometry. Talanta 72:373–377
Liu G, Chen H, Peng H, Song S, Gao J, Lu J, Ding M, Li L, Ren S, Zou Z, Fan C (2011) A carbon nanotube-based high-sensitivity electrochemical immunosensor for rapid and portable detection of clenbuterol. Biosens Bioelectron 28:308–313
Wang H, Liu X, He Y, Dong J, Sun Y, Liang Y, Yang J, Lei H, Shen Y, Xu X (2010) Expression and purification of an anti-clenbuterol single chain fv antibody in escherichia coli. Protein Expres Purif 72:26–31
Bacigalupo MA, Meroni G, Secundo F, Scalera C, Quici S (2009) Antibodies conjugated with new highly luminescent Eu3+ and Tb3+ chelates as markers for time resolved immunoassays. Application to simultaneous determination of clenbuterol and free cortisol in horse urine. Talanta 80:954–958
Zhu G, Hu Y, Gao J, Zhong L (2011) Highly sensitive detection of clenbuterol using competitive surface-enhanced raman scattering immunoassay. Anal Chim Acta 697:61–66
Sirichai S, Khanatharana P (2008) Rapid analysis of clenbuterol, salbutamol, procaterol, and fenoterol in pharmaceuticals and human urine by capillary electrophoresis. Talanta 76:1194–1198
Harkins JD, Woods WE, Lehner AF, Fisher M, Tobin T (2001) Clenbuterol in the horse: urinary concentrations determined by ELISA and GC/MS after clinical doses. J Vet Pharmacol Ther 24:7–14
Zhang QL, Li J, Ma TT, Zhang ZT (2008) Chemiluminescence screening assay for diethylstilbestrol in meat. Food Chem 111:498–502
Zhao C, Jin GP, Chen LL, Li Y, Yu B (2011) Preparation of molecular imprinted film based on chitosan/nafion/nano-silver/poly quercetin for clenbuterol sensing. Food Chem 129:595–600
Ross KA, Beaulieu AD, Merrill J, Vessie G, Patience JF (2011) The impact of ractopamine hydrochloride on growth and metabolism, with special consideration of its role on nitrogen balance and water utilization in pork production. J Anim Sci 89:2243–2256
Nguyen DN, Ngo TT, Nguyen QL (2012) Highly sensitive fluorescence resonance energy transfer (FRET)-based nanosensor for rapid detection of clenbuterol. Adv Nat Sci: Nanosci Nanotechnol 3:035011
Xu J, Li Y, Guo J, Shen F, Luo Y, Sun C (2014) Fluorescent detection of clenbuterol using fluorophore functionalized gold nanoparticles based on fluorescence resonance energy transfer. Food Control 46:67–74
Steiuwandter H (1989) Simple screening method for the fast determination of clenbuterol in animal feeds. Fresenius’ Z Anal Chem 333:634–636
Liu LC, Gao JG, Sun YH, Liu K, Jia SH, Li CC (2005) Micelle-enhanced inhibitory kinetic spectrophotometric determination of trace salicylic acid. Chin J Anal Lab 24:47–50
Acknowledgments
This work was supported by Fujian Province Natural Science Foundation (Grant No. 2010 J01053), Fujian Province Education Committee (JK2010035, JA11311, JA10203 and JA10277), Fujian provincial bureau of quality and technical supervison (FJQI2011006) and Scientific Research Program of Zhangzhou Institute of Technology Foundation (Grant No. ZZY1106 and ZZY1014). At the same time, we are very grateful to precious advices raised by the anonymous reviewers.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Liu, J., Liu, Zb., Huang, Q. et al. Highly Sensitive Fluorescent Probe for Clenbuterol Hydrochloride Detection Based on its Catalytic Oxidation of Eosine Y by NaIO4 . J Fluoresc 24, 1495–1501 (2014). https://doi.org/10.1007/s10895-014-1435-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10895-014-1435-7