Skip to main content
Log in

Highly Sensitive Fluorescent Probe for Clenbuterol Hydrochloride Detection Based on its Catalytic Oxidation of Eosine Y by NaIO4

  • ORIGINAL PAPER
  • Published:
Journal of Fluorescence Aims and scope Submit manuscript

Abstract

A highly sensitive fluorescent probe for clenbuterol hydrochloride (CLB) detection has been first designed based on its catalytic effect on NaIO4 oxidating eosine Y (R). And this environment-friendly, simple, rapid, selective and sensitive fluorescent probe has been utilized to detect CLB in the practical samples with the results consisting with those obtained by GC/MS. The structures of R and CLB were characterized by infrared spectra. The mechanism of the proposed assay for the detection of CLB was also discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Scheme 1
Scheme 2
Scheme 3
Scheme 4
Fig. 2

Similar content being viewed by others

References

  1. He P, Shen L, Liu R, Luo Z, Li Z (2011) Direct detection of β-agonists by use of gold nanoparticle-based colorimetric assays. Anal Chem 83:6988–6995

    Article  CAS  PubMed  Google Scholar 

  2. Nath N, Chilkoti A (2004) Label free colorimetric biosensing using nanoparticles. J Fluoresc 14:377–389

    Article  CAS  PubMed  Google Scholar 

  3. Parr MK, Opfermann G, Schänzer W (2009) Analytical methods for the detection of clenbuterol. Bioanalysis 1:437–450

    Article  CAS  PubMed  Google Scholar 

  4. Sharma D, Sahoo SK, Bera RK, Kamal R (2013) Spectroscopic and computational study of a naphthalene derivative as colorimetric and fluorescent sensor for bioactive anions. J Fluoresc 23:387–392

    Article  CAS  PubMed  Google Scholar 

  5. Gaichore RR, Srivastava AK (2012) Multiwalled carbon nanotube-4-tert-butyl calixarene composite electrochemical sensor for clenbuterol hydrochloride determination by means of differential pulse adsorptive stripping voltammetry. J Appl Electrochem 42:979–987

    Article  CAS  Google Scholar 

  6. Wang H, Zhang Y, Li H, Du B, Ma H, Wu D, Wei Q (2013) A silver-palladium alloy nanoparticle-based electrochemical biosensor for simultaneous detection of ractopamine, clenbuterol and salbutamol. Biosens Bioelectron 49:14–19

    Article  PubMed  Google Scholar 

  7. Evans RC, Douglas P, Williams JG, Rochester DL (2006) A novel luminescence-based colorimetric oxygen sensor with a “traffic light” response. J Fluoresc 16:201–206

    Article  CAS  PubMed  Google Scholar 

  8. Melwanki MB, Huang SD, Fuh MR (2007) Three-phase solvent bar microextraction and determination of trace amounts of clenbuterol in human urine by liquid chromatography and electrospray tandem mass spectrometry. Talanta 72:373–377

    Article  CAS  PubMed  Google Scholar 

  9. Liu G, Chen H, Peng H, Song S, Gao J, Lu J, Ding M, Li L, Ren S, Zou Z, Fan C (2011) A carbon nanotube-based high-sensitivity electrochemical immunosensor for rapid and portable detection of clenbuterol. Biosens Bioelectron 28:308–313

    Article  CAS  PubMed  Google Scholar 

  10. Wang H, Liu X, He Y, Dong J, Sun Y, Liang Y, Yang J, Lei H, Shen Y, Xu X (2010) Expression and purification of an anti-clenbuterol single chain fv antibody in escherichia coli. Protein Expres Purif 72:26–31

    Article  CAS  Google Scholar 

  11. Bacigalupo MA, Meroni G, Secundo F, Scalera C, Quici S (2009) Antibodies conjugated with new highly luminescent Eu3+ and Tb3+ chelates as markers for time resolved immunoassays. Application to simultaneous determination of clenbuterol and free cortisol in horse urine. Talanta 80:954–958

    Article  CAS  PubMed  Google Scholar 

  12. Zhu G, Hu Y, Gao J, Zhong L (2011) Highly sensitive detection of clenbuterol using competitive surface-enhanced raman scattering immunoassay. Anal Chim Acta 697:61–66

    Article  CAS  PubMed  Google Scholar 

  13. Sirichai S, Khanatharana P (2008) Rapid analysis of clenbuterol, salbutamol, procaterol, and fenoterol in pharmaceuticals and human urine by capillary electrophoresis. Talanta 76:1194–1198

    Article  CAS  PubMed  Google Scholar 

  14. Harkins JD, Woods WE, Lehner AF, Fisher M, Tobin T (2001) Clenbuterol in the horse: urinary concentrations determined by ELISA and GC/MS after clinical doses. J Vet Pharmacol Ther 24:7–14

    Article  CAS  PubMed  Google Scholar 

  15. Zhang QL, Li J, Ma TT, Zhang ZT (2008) Chemiluminescence screening assay for diethylstilbestrol in meat. Food Chem 111:498–502

    Article  CAS  Google Scholar 

  16. Zhao C, Jin GP, Chen LL, Li Y, Yu B (2011) Preparation of molecular imprinted film based on chitosan/nafion/nano-silver/poly quercetin for clenbuterol sensing. Food Chem 129:595–600

    Article  CAS  Google Scholar 

  17. Ross KA, Beaulieu AD, Merrill J, Vessie G, Patience JF (2011) The impact of ractopamine hydrochloride on growth and metabolism, with special consideration of its role on nitrogen balance and water utilization in pork production. J Anim Sci 89:2243–2256

    Article  CAS  PubMed  Google Scholar 

  18. Nguyen DN, Ngo TT, Nguyen QL (2012) Highly sensitive fluorescence resonance energy transfer (FRET)-based nanosensor for rapid detection of clenbuterol. Adv Nat Sci: Nanosci Nanotechnol 3:035011

    Google Scholar 

  19. Xu J, Li Y, Guo J, Shen F, Luo Y, Sun C (2014) Fluorescent detection of clenbuterol using fluorophore functionalized gold nanoparticles based on fluorescence resonance energy transfer. Food Control 46:67–74

    Article  CAS  Google Scholar 

  20. Steiuwandter H (1989) Simple screening method for the fast determination of clenbuterol in animal feeds. Fresenius’ Z Anal Chem 333:634–636

    Article  Google Scholar 

  21. Liu LC, Gao JG, Sun YH, Liu K, Jia SH, Li CC (2005) Micelle-enhanced inhibitory kinetic spectrophotometric determination of trace salicylic acid. Chin J Anal Lab 24:47–50

    Google Scholar 

Download references

Acknowledgments

This work was supported by Fujian Province Natural Science Foundation (Grant No. 2010 J01053), Fujian Province Education Committee (JK2010035, JA11311, JA10203 and JA10277), Fujian provincial bureau of quality and technical supervison (FJQI2011006) and Scientific Research Program of Zhangzhou Institute of Technology Foundation (Grant No. ZZY1106 and ZZY1014). At the same time, we are very grateful to precious advices raised by the anonymous reviewers.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jiaming Liu.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Liu, J., Liu, Zb., Huang, Q. et al. Highly Sensitive Fluorescent Probe for Clenbuterol Hydrochloride Detection Based on its Catalytic Oxidation of Eosine Y by NaIO4 . J Fluoresc 24, 1495–1501 (2014). https://doi.org/10.1007/s10895-014-1435-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10895-014-1435-7

Keywords

Navigation