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Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli

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Abstract

The aim of this study was the development of a non-toxic, biosynthetic antimicrobial agent which selectively acts on only one type of microorganism, and preserves the microbiota. Antimicrobial performance of biosynthesized silver nanoparticles (Ag NPs) by horsetail (Equisetum arvense L.) extract was examined against Gram-negative bacteria Escherichia coli and Gram-positive bacteria Staphylococcus aureus, as well as yeasts Candida albicans and Saccharomyces boulardii. Also, the cytotoxicity of Ag NPs was examined toward pre-osteoblast cells. The synthetic conditions—concentration of extract, temperature, and pH—were optimized to prepare silver colloids with different particle size distributions and long-term stability. The obtained samples were characterized using transmission electron microscopy, X-ray diffraction analysis, and absorption spectroscopy. The smaller-sized Ag NPs (~ 10–20 nm), prepared at a lower temperature (20 °C), showed better antimicrobial performance against E. coli compared to larger ones (~ 40–60 nm), prepared at high temperature (100 °C). On the other hand, both samples did not display any toxic action against bacteria S. aureus, or yeasts C. albicans and S. boulardii. Non-cytotoxic behavior of Ag NPs toward pre-osteoblast cells was observed for the concentrations of silver ≤ 2.25 and ≤ 4.5 mg L−1 for 10–20 and 40–60 nm-sized Ag NPs, respectively. Biosynthesized Ag NPs by horsetail extract display selective toxic action against E. coli at the ecologically acceptable concentration level.

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References

  1. C.J. Murphy, T.K. Sau, A.M. Gole, C.J. Orendorff, J. Gao, L. Gou, S.E. Hunyadi, T. Li, Anisotropic metal nanoparticles: synthesis, assembly, and optical applications. J. Phys. Chem. B 109, 13857–13870 (2005)

    Article  CAS  PubMed  Google Scholar 

  2. A. Panaček, L. Kvitek, R. Prucek, M. Kolar, R. Večerova, N. Pizurova, V.K. Sharma, T. Nevečna, R. Zboril, Silver colloid nanoparticles: synthesis, characterization, and their antibacterial activity. J. Phys. Chem. B 110, 16248–16253 (2006)

    Article  PubMed  CAS  Google Scholar 

  3. S. Pal, Y.K. Tak, J.M. Song, Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the Gram-negative bacterium Escherichia coli. Appl. Environ. Microb. 73, 1712–1720 (2007)

    Article  CAS  Google Scholar 

  4. M. Rai, A. Yadav, A. Gade, Silver nanoparticles as a new generation of antimicrobials. Biotechnol. Adv. 27, 76–83 (2009)

    Article  CAS  PubMed  Google Scholar 

  5. V. Ilić, Z.V. Šaponjić, V.V. Vodnik, B. Potkonjak, P. Jovančić, J.M. Nedeljković, M. Radetić, The influence of silver content on antimicrobial activity and color of cotton fabrics functionalized with Ag nanoparticles. Carbohyd. Polym. 78, 564–569 (2009)

    Article  CAS  Google Scholar 

  6. M. Radetić, V. Ilić, V.V. Vodnik, S. Dimitrijević, P. Jovančić, Z.V. Šaponjić, J.M. Nedeljković, Antibacterial effect of silver nanoparticles deposited on corona-treated polyester and polyamide fabrics. Polym. Adv. Technol. 19, 1816–1821 (2008)

    Article  CAS  Google Scholar 

  7. V. Ilić, Z. Šaponjić, V. Vodnik, D. Mihailović, P. Jovančić, M. Radetić, A study of the antibacterial efficiency and coloration of dyed polyamide and polyester fabrics modified with colloidal Ag nanoparticles. J. Serb. Chem. Soc. 74, 349–357 (2009)

    Article  CAS  Google Scholar 

  8. Y.-M. Cho, Y. Mizuta, J.-I. Akagi, T. Toyoda, M. Sone, K. Ogawa, Size-dependent acute toxicity of silver nanoparticles in mice. J. Toxicol. Pathol. 31, 73–80 (2018)

    Article  CAS  PubMed  Google Scholar 

  9. R. Vazquez-Muñoz, B. Borrego, K. Juárez-Moreno, M. García-García, J.D. Mota Morales, N. Bogdanchikova, A. Huerta-Saquero, Toxicity of silver nanoparticles in biological systems: does the complexity of biological systems matter? Toxicol. Lett. 276, 11–20 (2017)

    Article  PubMed  CAS  Google Scholar 

  10. V.V. Vukovic, J.M. Nedeljkovic, Surface modification of nanometer-scale silver particles by imidazole. Langmuir 9, 980–983 (1993)

    Article  CAS  Google Scholar 

  11. S. Onitsuka, T. Hamada, H. Okamura, Preparation of antimicrobial gold and silver nanoparticles from tea leaf extracts. Colloids Surf. B 173, 242–248 (2019)

    Article  CAS  Google Scholar 

  12. M. Chen, Y.G. Feng, X. Wang, T.C. Li, J.Y. Zhang, D.J. Qian, Silver nanoparticles capped by oleylamine: formation, growth, and self-organization. Langmuir 23, 5296–5304 (2007)

    Article  CAS  PubMed  Google Scholar 

  13. C. Marambio-Jones, E.M.V. Hoek, A review of the antibacterial effects of silver nanomaterials and potential emplications for human health and the environment. J. Nanopart. Res. 12, 1531–1551 (2010)

    Article  CAS  Google Scholar 

  14. X. Dong, X. Ji, J. Jing, M. Li, J. Li, W. Yang, Synthesis of triangular silver nanoprisms by stepwise reduction of sodium borohydride and trisodium citrate. J. Phys. Chem. C 114, 2070–2074 (2010)

    Article  CAS  Google Scholar 

  15. Z. Shan, J. Wu, F. Xu, F.-Q. Huang, H. Ding, Highly effective silver/semiconductor photocatalytic composites prepared by a silver mirror reaction. J. Phys. Chem. C 112, 15423–15428 (2008)

    Article  CAS  Google Scholar 

  16. B. Pietrobon, M. McEachran, V. Kitaev, Synthesis of size-controlled faceted pentagonal silver nanorods with tunable plasmonic properties and self-assembly of these nanorods. ACS Nano 3, 21–26 (2009)

    Article  CAS  PubMed  Google Scholar 

  17. B. Wiley, Y. Sun, Y. Xia, Synthesis of silver nanostructures with controlled shapes and properties. Acc. Chem. Res. 40, 1067–1076 (2007)

    Article  CAS  PubMed  Google Scholar 

  18. W.R. Rolim, M.T. Pelegrino, B. de Araújo Lima, L.S. Ferraz, F.N. Costa, J.S. Bernardes, T. Rodigues, M. Brocchi, A.B. Seabra, Green tea extract mediated biogenic synthesis of silver nanoparticles: characterization, cytotoxicity evaluation and antibacterial activity. Appl. Surf. Sci. 463, 66–74 (2019)

    Article  CAS  Google Scholar 

  19. N. Durán, P.D. Marcato, M. Durán, A. Yadav, A. Gade, M. Rai, Mechanistic aspects in the biogenic synthesis of extracellular metal nanoparticles by peptides, bacteria, fungi, and plants. Appl. Microbiol. Biotechnol. 90, 1609–1624 (2011)

    Article  PubMed  CAS  Google Scholar 

  20. N. Durán, A.B. Seabra, Metallic oxide nanoparticles: state of the art in biogenic syntheses and their mechanisms. Appl. Microbiol. Biotechnol. 95, 275–288 (2012)

    Article  PubMed  CAS  Google Scholar 

  21. N. Durán, A.B. Seabra, Biogenic synthesized Ag/Au nanoparticles: production, characterization, and applications. Curr. Nanosci. 14, 82–94 (2018)

    Article  CAS  Google Scholar 

  22. S. Davidović, V. Lazić, I. Vukoje, J. Papan, S.P. Anhrenkiel, S. Dimitrijević, J.M. Nedeljković, Dextran coated silver nanoparticles—chemical sensor for selective cysteine detection. Colloids Surf. B 160, 184–191 (2017)

    Article  CAS  Google Scholar 

  23. S. Pirtarighat, M. Ghannadnia, S. Baghshahi, Green synthesis of silver nanoparticles using the plant extract of Salvia spinosa grown in vitro and their antibacterial activity assessment. J. Nanostruct. Chem. 9, 1–9 (2019)

    Article  CAS  Google Scholar 

  24. M. Bhagat, R. Anand, R. Datt, V. Gupta, S. Arya, Green synthesis of silver nanoparticles using aqueous extract of Rosa brunonii Lindl and their morphological, biological and photocatalytic characterizations. J. Inorg. Organomet. Polym. 29, 1039–1047 (2019)

    Article  CAS  Google Scholar 

  25. M. Akter, M.M. Rahman, A.K.M.A. Ullah, M.T. Sikder, T. Hosokawa, T. Saito, M. Kurasaki, Brassica rapa var. japonica leaf extract mediated green synthesis of crystalline silver nanoparticles and evaluation of their stability, cytotoxicity and antibacterial activity. J. Inorg. Organomet. Polym. 28, 1483–1493 (2018)

    Article  CAS  Google Scholar 

  26. Y. Gavamukulya, E.N. Maina, A.M. Meroka, E.S. Madivoli, H.A. El-Shemy, F. Wamunyokoli, G. Magoma, Green synthesis and characterization of highly stable silver nanoparticles from ethanolic extracts of fruits of Annona muricata. J. Inorg. Organomet. Polym. (2019). https://doi.org/10.1007/s10904-019-01262-5

    Article  Google Scholar 

  27. L. Huang, Y. Sun, S. Mahmud, H. Liu, Biological and environmental applications of silver nanoparticles synthesized using the aqueous extract of Gingo biloba leaf. J. Inorg. Organomet. Polym. (2019). https://doi.org/10.1007/s10904-019-01313-x

    Article  Google Scholar 

  28. P. Velusamy, J. Das, R. Pachaiappan, B. Vaseeharan, K. Pandian, Greener approach for synthesis of antibacterial silver nanoparticles using aqueous solution of neem gum (Azadirachta indica L.). Ind. Crop. Prod. 66, 103–109 (2015)

    Article  CAS  Google Scholar 

  29. R. Sood, D.S. Chopra, Optimization of reaction conditions to fabricate Ocimum sanctum synthesized silver nanoparticles and its application to nano-gel systems for burn wounds. Mater. Sci. Eng. C 92, 575–589 (2018)

    Article  CAS  Google Scholar 

  30. R. Rajan, K. Chandran, S.L. Harper, S.-I. Yun, P.T. Kalaichelvan, Plant extract synthesized silver nanoparticles: an ongoing source of novel biocompatible materials. Ind. Crop. Prod. 70, 356–373 (2015)

    Article  CAS  Google Scholar 

  31. J.Y. Song, B.S. Kim, Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioproc. Biosyst. Eng. 32, 79 (2008)

    Article  CAS  Google Scholar 

  32. S. Ahmed, M. Ahmad, B.L. Swami, S. Ikram, A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: a green expertise. J. Adv. Res. 7, 17–28 (2016)

    Article  CAS  PubMed  Google Scholar 

  33. M. Milutinović, N. Radovanović, M. Rajilić-Stojanović, S. Šiler-Marinković, S. Dimitrijević, S. Dimitrijević-Branković, Microwave-assisted extraction for the recovery of antioxidants from waste Equisetum arvense. Ind. Crop. Prod. 61, 388–397 (2014)

    Article  CAS  Google Scholar 

  34. F.C.H.M. Do Monte, J.G. dos Santos, M. Russi, V.M.N. Bispo Lanziotti, L.K.A.M. Leal, G.M. de Andrade Cunha, Antinociceptive and anti-inflammatory properties of the hydroalcoholic extract of stems from Equisetum arvense L. in mice. Pharmacol. Res. 49, 239–243 (2004)

    Article  PubMed  Google Scholar 

  35. N.S. Sandhu, S. Kaur, D. Chopra, Equietum arvense: pharmacology and phytochemistry—a review. Asian. J. Pharm. Clin. Res. 3, 146–150 (2010)

    CAS  Google Scholar 

  36. R. Stefanović, Paradigma održivog razvoja poljoprivrede - strateški koncept zaštite životne sredine. Ecologica 17, 112–114 (2010)

    Google Scholar 

  37. J.M. Čanadanović-Brunet, G.S. Ćetković, S.M. Djilas, V.T. Tumbas, S.S. Savatović, A.I. Mandić, S.L. Markov, D.D. Cvetković, Radical scavenging and antimicrobial activity of horsetail (Equisetum arvense L.) extracts. Int. J. Food Sci. Technol. 44, 269–278 (2009)

    Article  CAS  Google Scholar 

  38. K.P. Bankura, D. Maity, M.M.R. Mollick, D. Mondal, B. Bhowmick, M.K. Bain, A. Chakraborty, J. Sarkar, K. Acharya, D. Chattopadhyay, Synthesis, characterization and antimicrobial activity of dextran stabilized silver nanoparticles in aqueous medium. Carbohyd. Polym. 89, 1159–1165 (2012)

    Article  CAS  Google Scholar 

  39. J.M. Herrero-Martinez, M. Sanmartin, M. Roses, E. Bosch, C. Rafols, Determination of dissociation constants of flavonoids by capillary electrophoresis. Electrophoresis 26, 1886–1895 (2005)

    Article  CAS  PubMed  Google Scholar 

  40. N. Sauerwald, M. Schwenk, J. Polster, E. Bengsch, Spectrometric pK determination of daphnetin, chlorogenic acid and quercetin. Zeitschrift fur naturforschung B 53(3), 315–321 (1998)

    Article  CAS  Google Scholar 

  41. K.L. Kelly, E. Coronado, L.L. Zhao, G.C. Schatz, The optical properties of metal nanoparticles: the influence of size, shape, and dielectric environment. J. Phys. Chem. B 107, 668–677 (2003)

    Article  CAS  Google Scholar 

  42. T. Huang, X.-H.N. Xu, Synthesis and characterization of tunable rainbow colored colloidal silver nanoparticles using single-nanoparticle plasmonic microscopy and spectroscopy. J. Mater. Chem. 20, 9867–9876 (2010)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  43. P. Anbu, S.C.B. Gopinath, H.S. Yun, C.-G. Lee, Temperature-dependent green biosynthesis and characterization of silver nanoparticles using balloon flower plants and their antibacterial potential. J. Mol. Struct. 1177, 302–309 (2019)

    Article  CAS  Google Scholar 

  44. C.N. Lok, C.M. Ho, M. Chen, Q.Y. He, W.Y. Yu, H. Sun, P.K.H. Tam, J.F. Chiu, C.M. Che, Silver nanoparticles: partial oxidation and antibacterial activities. J. Biol. Inorg. Chem. 12, 527–534 (2007)

    Article  CAS  PubMed  Google Scholar 

  45. J.R. Morones, J.L. Elechiguerra, A. Camacho, K. Holt, J.N. Kouri, J.T. Ramirez, M.J. Yacaman, The antibacterial effect of silver nanoparticles. Nanotechnology 16, 2346 (2005)

    Article  CAS  PubMed  Google Scholar 

  46. Z.M. Xiu, J. Mao, P.J.J. Alvarez, Differential effect of common ligands and molecular oxygen on antimicrobial activity of silver nanoparticles versus silver ions. Environ. Sci. Technol. 45, 9003–9008 (2011)

    Article  CAS  PubMed  Google Scholar 

  47. L. Wang, C. Hu, L. Shao, The antimicrobial activity of nanoparticles: present situation and prospects for the future. Int. J. Nanomed. 12, 1227–1249 (2017)

    Article  CAS  Google Scholar 

  48. D.H. Kim, J.C. Park, G.E. Jeon, C.S. Kim, J.H. Seo, Effect of the size and shape of silver nanoparticles on bacterial growth and metabolism by monitoring optical density and fluorescence intensity. Biotechnol. Bioproc. Eng. 22, 210–217 (2017)

    Article  CAS  Google Scholar 

  49. M.A. Raza, Z. Kanwal, A. Rauf, A.N. Sabri, S. Riaz, S. Naseem, Size- and shape-dependent antibacterial studies of silver nanoparticles synthesized by Wet chemical routes. Nanomaterials (Basel, Switzerland) 6, 74 (2016)

    Article  CAS  Google Scholar 

  50. I. Vukoje, E. Džunuzović, V. Vodnik, S. Dimitrijević, S.P. Ahrenkiel, J.M. Nedeljković, Synthesis, characterization, and antimicrobial activity of poly(GMA-co-EGDMA) polymer decorated with silver nanoparticles. J. Mater. Sci. 49, 6838–6844 (2014)

    Article  CAS  Google Scholar 

  51. I. Vukoje, E. Džunuzović, D. Lončarević, S. Dimitrijević, S.P. Ahrenkiel, J.M. Nedeljković, Synthesis, characterization, and antimicrobial activity of silver nanoparticles on poly(GMA-co-EGDMA) polymer support. Polym. Compos. 38, 1206–1214 (2017)

    Article  CAS  Google Scholar 

  52. V. Lazić, I. Smičiklas, J. Marković, D. Lončarević, J. Dostanić, S.P. Ahrenkiel, J.M. Nedeljković, Antibacterial ability of supported silver nanoparticles by functionalized hydroxyapatite with 5-aminosalicylic acid. Vacuum 148, 62–68 (2018)

    Article  CAS  Google Scholar 

  53. V. Lazić, K. Mihajlovski, A. Mraković, E. Illés, M. Stoiljković, S.P. Ahrenkiel, J.M. Nedeljković, Antimicrobial activity of silver nanoparticles supported by magnetite. ChemistrySelect 4, 4018–4024 (2019)

    Article  CAS  Google Scholar 

  54. C. Cattò, E. Garuglieri, L. Borruso, D. Erba, M.C. Casiraghi, F. Cappitelli, F. Villa, S. Zecchin, R. Zanchi, Impacts of dietary silver nanoparticles and probiotic administration on the microbiota of an in vitro gut model. Environ. Pollut. 245, 754–763 (2019)

    Article  PubMed  CAS  Google Scholar 

  55. M. Panaček, R. Kolar, R. Večerova, J. Prucek, V. Soukupova, P. Kryštof, R. Hamal, L. Zboril, L. Kvitek, Antifungal activity of silver nanoparticles against Candida spp. Biomaterials 30, 6333–6340 (2009)

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

Financial support for this study was granted by the Ministry of Education, Science and Technological Development of the Republic of Serbia (Projects III 45020 and TR 31035).

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Authors and Affiliations

  1. Department of Biochemical Engineering and Biotechnology, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade, 11120, Serbia

    Miona Miljković & Slađana Davidović

  2. Vinča Institute of Nuclear Sciences, University of Belgrade, P.O. Box 522, Belgrade, 11001, Serbia

    Vesna Lazić, Jelena Papan & Jovan M. Nedeljković

  3. Department of Biochemical Engineering and Biotechnology, Center of Innovation, Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade, 11120, Serbia

    Ana Milivojević

  4. Centre of Physics, University of Minho, 4710-057, Braga, Portugal

    Margarida M. Fernandes

  5. Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal

    Margarida M. Fernandes

  6. BCMaterials, Basque Center for Materials, Applications and Nanostructures, UPV/EHU Science Park, 48940, Leioa, Spain

    Senentxu Lanceros-Mendez

  7. Ikerbasque, Basque Foundation for Science, 48013, Bilbao, Spain

    Senentxu Lanceros-Mendez

  8. South Dakota School of Mines and Technology, 501 E. Saint Joseph Street, Rapid City, SD, 57701, USA

    S. Phillip Ahrenkiel

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  1. Miona Miljković
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  2. Vesna Lazić
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  9. Jovan M. Nedeljković
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Miljković, M., Lazić, V., Davidović, S. et al. Selective Antimicrobial Performance of Biosynthesized Silver Nanoparticles by Horsetail Extract Against E. coli. J Inorg Organomet Polym 30, 2598–2607 (2020). https://doi.org/10.1007/s10904-019-01402-x

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