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Casein kinase 2 inhibition differentially modulates apoptotic effect of trichostatin A against epithelial ovarian carcinoma cell lines

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Abstract

Histone deacetylase inhibitors and casein kinase 2 inhibitors have been shown to induce apoptosis. However, the combined effect of casein kinase 2 inhibition on the apoptotic effect of histone deacetylase inhibitor is unknown. We assessed the effect of casein kinase 2 inhibition on the apoptotic effect of trichostatin A in human epithelial carcinoma cell lines with respect to cell death signaling pathways. At concentrations that did not induce cell death, the casein kinase 2 inhibitor 4,5,6,7-tetrabromobenzotriazole inhibited activation of apoptotic proteins and changes in mitochondrial membrane permeability induced by the histone deacetylase inhibitor trichostatin A. These results suggest that casein kinase 2 inhibition may reduce trichostatin A-induced apoptosis in ovarian carcinoma cell lines by suppressing activation of apoptotic proteins and changes in mitochondrial membrane permeability, which both lead to caspase-3 activation. Casein kinase 2 inhibition, which does not induce a cytotoxic effect, may prevent histone deacetylase inhibitor-mediated apoptosis.

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Abbreviations

TBB:

4,5,6,7-tetrabromobenzotriazole

MTT:

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

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Acknowledgments

This study was supported by a grant of the Korea Healthcare Technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (A085138).

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Authors and Affiliations

  1. Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul, 156-756, South Korea

    Chung Soo Lee, Eun-Ra Jang & Yun Jeong Kim

  2. Department of Urology, Chung-Ang University Hospital, Seoul, 156-755, South Korea

    Soon Chul Myung

  3. Department of Microbiology, College of Medicine, Chung-Ang University, Seoul, 156-756, South Korea

    Wonyong Kim

  4. Research Institute for Translational System Bionomics, College of Medicine, Chung-Ang University, Seoul, 156-756, South Korea

    Wonyong Kim

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  1. Chung Soo Lee
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Correspondence to Chung Soo Lee.

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Lee, C.S., Jang, ER., Kim, Y.J. et al. Casein kinase 2 inhibition differentially modulates apoptotic effect of trichostatin A against epithelial ovarian carcinoma cell lines. Mol Cell Biochem 338, 157–166 (2010). https://doi.org/10.1007/s11010-009-0349-1

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  • DOI: https://doi.org/10.1007/s11010-009-0349-1

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