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Inherent anti-HIV activity of biocompatible anionic citrate-PEG-citrate dendrimer

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Abstract

The development of new combinations to empower better protection against HIV infection is particularly important. Anionic polymers can block HIV infection. In the current study, first generation (G1) and second generation (G2) novel water-soluble anionic citrate-PEG-citrate dendrimers were synthesized and characterized with Fourier-transform infrared spectroscopy (FTIR), nuclear magnetic resonance spectroscopy (NMR), and dynamic light scattering (DLS) methods. After the biocompatibility of the G2 dendrimer was determined, its antiviral activity was evaluated. This function may contribute to the peripheral groups of this dendrimer (carboxylate group). In order to measure the inhibitory effect of G2 on HIV infection, both pre-treatment (treated with G2 dendrimer before HIV infection) and co-treatment (simultaneously treated with G2 dendrimer and HIV infection) were used in vitro. The results showed the good synthesis of the G2 dendrimer, and the dendrimer showed antiviral properties (ICC50:0.4 mM) and low toxicity (CC50:0.6 mM) at high concentrations. A strong inhibitory effect was found when the co-treatment approach was used. This study achieved promising results which encourage the use of G2 dendrimers as anti-HIV agents.

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Authors and Affiliations

  1. Department of Life Science Engineering, Faculty of New Science and Technology, University of Tehran, Tehran, Iran

    Mohammad Reza Kandi & Javad Mohammadnejad

  2. Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

    Mehdi Shafiee Ardestani

  3. Hepatitis and AIDS Department, Pasteur Institute of Iran, P. O. Box 14115-331, Tehran, Iran

    Rezvan Zabihollahi, Sepehr Soleymani, Mohammad Reza Aghasadeghi & Kazem Baesi

Authors
  1. Mohammad Reza Kandi
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  2. Javad Mohammadnejad
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  3. Mehdi Shafiee Ardestani
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  4. Rezvan Zabihollahi
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  5. Sepehr Soleymani
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  6. Mohammad Reza Aghasadeghi
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  7. Kazem Baesi
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Correspondence to Kazem Baesi.

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Kandi, M.R., Mohammadnejad, J., Shafiee Ardestani, M. et al. Inherent anti-HIV activity of biocompatible anionic citrate-PEG-citrate dendrimer. Mol Biol Rep 46, 143–149 (2019). https://doi.org/10.1007/s11033-018-4455-6

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  • DOI: https://doi.org/10.1007/s11033-018-4455-6

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