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Suppression of lncRNA NORAD may affect cell migration and apoptosis in gastric cancer cells

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Abstract

Background

Gastric cancer (GC) is a major malignancy that threatens people’s lives worldwide. Long noncoding RNA (lncRNA) non-coding RNA activated by DNA damage (NORAD) is known to be a potential oncogene in many cancers and may promote cell migration and metastasis, and decrease apoptosis rate.

Material and methods

NORAD expression was measured in 70 pairs of GC tissues and their adjacent normal tissues (ANTs) by quantitative real-time polymerase chain reaction. Si-NORAD gene knockdown study and cellular assays were conducted to assess the correlation between NORAD expression and cell viability, apoptosis, migration, and metastasis.

Results

NORAD was significantly overexpressed in GC tissues compared to ANTs (P value < 0.0001). The receiver operating characteristic curve indicated the AUC of 0.721 with the sensitivity and specificity of 78.57 and 61.43, respectively (P value < 0.0001). NORAD downregulation leads to decreased cell viability (P value < 0.001) and migration (P value < 0.01), increased apoptosis rate (P value < 0.0001), and increased protein level for PTEN, E-cadherin, and Bax, but decreased protein level for Bcl-2.

Conclusion

Generally, NORAD may serve as a potential diagnostic biomarker in GC.

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Data availability

The data are available on reasonable request.

Code availability

REST, GraphPad Prism v.8, SPSS 16.0, ImageJ1.48 V.

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Acknowledgements

We are grateful to the patients, staff, and nurses in ARAS clinic, Imam Khomeini Hospital, Ardabil, Iran.

Funding

The present study was supported by Iran National Science Foundation (Grant no. 97014232).

Author information

Authors and Affiliations

  1. Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, 5166616471, Tabriz, Iran

    Negin Raei, Reza Safaralizadeh & Mohammadali Hosseinpourfeizi

  2. Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran

    Saeid Latifi-Navid

  3. Digestive Disease Research Center, Ardabil University of Medical Sciences, Ardabil, Iran

    Abbas Yazdanbod

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  1. Negin Raei
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  4. Saeid Latifi-Navid
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Contributions

RS provided direction in the preparation of the manuscript, NR wrote the first draft of the manuscript. SL-N and NR analyzed the data, SL-N, MH, and AY discussed and revised the manuscript, and finally, RS approved the version to be published.

Corresponding author

Correspondence to Reza Safaralizadeh.

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Conflict of interest

The authors declare no conflict of interest.

Ethical approval

The present study was approved by the Ethics Committee of the Digestive Disease Research Center (DDRC), Ardabil, Iran/ IR.ARUMS.REC.1397.181.

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Raei, N., Safaralizadeh, R., Hosseinpourfeizi, M. et al. Suppression of lncRNA NORAD may affect cell migration and apoptosis in gastric cancer cells. Mol Biol Rep 49, 3289–3296 (2022). https://doi.org/10.1007/s11033-022-07167-w

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  • DOI: https://doi.org/10.1007/s11033-022-07167-w

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