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Utility of six fungicides for selective isolation of Evlachovaea sp. and Tolypocladium cylindrosporum

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Abstract

The effects of the fungicides dodine, benomyl, thiabendazole, mancozeb, cupric sulfate, and copper oxychloride were examined in vitro upon germination and further development of Evlachovaea sp. and Tolypocladium cylindrosporum. Fungicidal activity depended on concentrations and varied among products, fungi and the strains tested. Depending on the fungicidal concentration, germination of conidia was induced but germlings produced neither mycelium nor new conidia. There was a good recovery of both Evlachovaea sp. and T. cylindrosporum from previously sterilized soils with fungicide-supplemented medium. Fungi were resistant to copper oxychloride up to 30 g/l, and this fungicide was found to have no utility for a selective medium. Minimal fungicide concentrations for successful isolations were 1 mg/l for benomyl, 200 mg/l for cupric sulfate, 50 mg/l for dodine, 100 mg/l for mancozeb, and 4 mg/l for thiabendazole. Thiabendazole, which is easy to obtain and can be used in low quantities, showed the greatest utility for a selective medium with these entomopathogenic fungi.

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Acknowledgments

The authors thank Richard A. Humber for providing T. cylindrosporum strains, identifying Evlachovaea sp. and reviewing the manuscript; and the National Council of Scientific and Technological Development (CNPq, Brazil) for financial support.

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Authors and Affiliations

  1. DMIPP, Instituto de Patologia Tropical e Saúde Pública (IPTSP), Universidade Federal de Goiás (UFG), CP 131, 74001-970, Goiânia, GO, Brazil

    Luiz F. N. Rocha & Christian Luz

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  1. Luiz F. N. Rocha
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  2. Christian Luz
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Correspondence to Christian Luz.

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Rocha, L.F.N., Luz, C. Utility of six fungicides for selective isolation of Evlachovaea sp. and Tolypocladium cylindrosporum . Mycopathologia 167, 341–350 (2009). https://doi.org/10.1007/s11046-009-9186-8

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  • DOI: https://doi.org/10.1007/s11046-009-9186-8

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