Abstract
In the developed countries infections of the feet (tinea pedis, athlete’s foot) and nails (onychomycosis) with the anthropophile fungus Trichophyton rubrum are most common. We examined the propagation of dermatophytes before and during domestic laundering. About 10% of the infectious material was transferred from contaminated textiles to sterile textiles during storage in a clothes basket simulation indicating a high infection risk during storage. This was evaluated with two quantification techniques: cultivation with subsequent colony counting and tracing of radioactively labelled propagating units. Both approaches reliably revealed similar results with the latter method reducing experimental time to few minutes compared to 2 weeks with the traditional method. The tracer technique allowed favourably to directly reflect the textile-bound infectious material at the moment of skin contact. To address the infection risk during domestic laundry, bioindicators with T. rubrum or the yeast Candida albicans were introduced into common domestic washing procedures with different temperature courses. While C. albicans did not survive any of the tests, T. rubrum could be recovered after washing at 30°C, indicating the risk potential of dermatophyte infections at home. Up to 16% of the initial fungus load was detected in the rinsing water. Washing at 60°C however, eliminated both pathogens, T. rubrum and C. albicans.
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Vermout S, Tabart J, Baldo A, Mathy A, Losson B, Mignon B. Pathogenesis of dermatophytosis. Mycopathologia. 2008;166(5–6):267–75.
Howard DH, Weitzman I, Padhye AA. Onygenales: Arthrodermataceae. In: Howard DH, editor. Pathogenic fungi in humans and animals. New York, USA: Marcel Dekker; 2003. p. 141–94.
Weitzman I, Summerbell RC. The dermatophytes. Clin Microbiol Rev. 1995;8(2):240–59.
Degreef H. Clinical forms of dermatophytosis (ringworm infection). Mycopathologia. 2008;166(5–6):257–65.
Kwon-Chung K, Bennett J. Medical mycology. Malvern: Lea and Febiger; 1992.
Jayatilake JA, Tilakaratne WM, Panagoda GJ. Candidal onychomycosis: a mini-review. Mycopathologia. 2009;168(4):165–73.
Chen BK, Friedlander SF. Tinea capitis update: a continuing conflict with an old adversary. Curr Opin Pediatr. 2001;13(4):331–5.
Seebacher C, Bouchara JP, Mignon B. Updates on the epidemiology of dermatophyte infections. Mycopathologia. 2008;166(5–6):335–52.
Sentamilselvi G, Janaki C, Kamalam A, Thambiah AS. Deep dermatophytosis caused by Trichophyton rubrum—a case report. Mycopathologia. 1998;142(1):9–11.
Squeo RF, Beer R, Silvers D, Weitzman I, Grossman M. Invasive Trichophyton rubrum resembling blastomycosis infection in the immunocompromised host. J Am Acad Dermatol. 1998;39(2 Pt 2):379–80.
Smith KJ, Welsh M, Skelton H. Trichophyton rubrum showing deep dermal invasion directly from the epidermis in immunosuppressed patients. Br J Dermatol. 2001;145(2):344–8.
Seckin D, Arikan S, Haberal M. Deep dermatophytosis caused by Trichophyton rubrum with concomitant disseminated nocardiosis in a renal transplant recipient. J Am Acad Dermatol. 2004;51(5 Suppl):S173–6.
Gupta AK, Ahmad I, Porretta M, Summerbell RC. Arthroconidial formation in Trichophyton raubitschekii. Mycoses. 2003;46(8):322–8.
Kane J. Laboratory handbook of dermatophytes: a clinical guide and laboratory handbook of dermatophytes and other filamentous fungi from skin, hair, and nails. Belmont: Star Pub; 1997.
Watanabe K, Taniguchi H, Katoh T. Adhesion of dermatophytes to healthy feet and its simple treatment. Mycoses. 2000;43(1–2):45–50.
Chen SC, Sorrell TC. Antifungal agents. Med J Aust. 2007;187(7):404–9.
Gupta AK, Ryder JE, Chow M, Cooper EA. Dermatophytosis: the management of fungal infections. Skinmed. 2005;4(5):305–10.
Mota CR, Miranda KC, Lemos Jde A, Costa CR, Hasimoto e Souza LK, Passos XS, et al. Comparison of in vitro activity of five antifungal agents against dermatophytes, using the agar dilution and broth microdilution methods. Rev Soc Bras Med Trop. 2009;42(3):250–4.
Robert R, Pihet M. Conventional methods for the diagnosis of dermatophytosis. Mycopathologia. 2008;166(5–6):295–306.
Kanbe T. Molecular approaches in the diagnosis of dermatophytosis. Mycopathologia. 2008;166(5–6):307–17.
Field LA, Adams BB. Tinea pedis in athletes. Int J Dermatol. 2008;47(5):485–92.
Andrews MD, Burns M. Common tinea infections in children. Am Fam Physician. 2008;77(10):1415–20.
Tanaka K, Katoh T, Irimajiri J, Taniguchi H, Yokozeki H. Preventive effects of various types of footwear and cleaning methods on dermatophyte adhesion. J Dermatol. 2006;33(8):528–36.
Ossowski B, Duchmann U. Effect of domestic laundry processes on mycotic contamination of textiles. Hautarzt. 1997;48(6):397–401.
Stellaard F. Use of dual isotope tracers in biomedical research. Isotopes Environ Health Stud. 2005;41(3):275–86.
Mesnard F, Ratcliffe RG. NMR analysis of plant nitrogen metabolism. Photosynth Res. 2005;83(2):163–80.
Grimble GK, Malik SB, Boza JJ. Methods for measuring tissue RNA turnover. Curr Opin Clin Nutr Metab Care. 2000;3(5):399–408.
Rapoport SI. In vivo approaches and rationale for quantifying kinetics and imaging brain lipid metabolic pathways. Prostaglandins Other Lipid Mediat. 2005;77(1–4):185–96.
Paulus TJ, Cramer CL, Davis RH. Compartmentation of spermidine in Neurospora crassa. J Biol Chem. 1983;258(14):8608–12.
Gieseke A, Nielsen JL, Amann R, Nielsen PH, de Beer D. In situ substrate conversion and assimilation by nitrifying bacteria in a model biofilm. Environ Microbiol. 2005;7(9):1392–404.
van Rooijen N. Labelling of lymphocytes with various radioisotopes for in vivo tracer studies; a review. J Immunol Methods. 1977;15(3):267–77.
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The authors thank Anne te Boekhorst, Melanie Deigner and Monique Siegel, Hohenstein Institutes, for their technical support.
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Hammer, T.R., Mucha, H. & Hoefer, D. Infection Risk by Dermatophytes During Storage and After Domestic Laundry and Their Temperature-Dependent Inactivation. Mycopathologia 171, 43–49 (2011). https://doi.org/10.1007/s11046-010-9347-9
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DOI: https://doi.org/10.1007/s11046-010-9347-9