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Serum microRNA is a biomarker for post-operative monitoring in glioma

  • Clinical Study
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A Correction to this article was published on 01 September 2020

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Abstract

Purpose

A circulating biomarker has potential to provide more accurate information for glioma progression post treatment, however no such biomarker is currently available. We aimed to discover a microRNA serum biomarker for longitudinal monitoring of glioma patients.

Methods

A prospectively collected cohort of 91 glioma patients and 17 healthy controls underwent pre and post-operative serum miRNA profiling using Nanostring®. Differentially expressed miRNAs were discovered using a machine learning random forest analysis. Candidate miRNAs were then assessed by droplet digital PCR in 11 patients with multiple follow up samples and compared to tumor volume based on magnetic resonance imaging.

Results

A 9-gene miRNA signature was identified that could distinguish between glioma and healthy controls with 99.8% accuracy. Two miRNAs miR-223 and miR-320e, best demonstrated dynamic changes that correlated closely with tumor volume in LGG and GBM respectively. Importantly, miRNA levels did not increase in two cases of pseudo-progression, indicating the potential utility of this test in guiding treatment decisions.

Conclusions

We identified a highly accurate 9-miRNA signature associated with glioma serum. Additionally, we observed dynamic changes in specific miRNAs correlating with tumor volume over long-term follow up. These results support a large prospective validation study of serum miRNA biomarkers in glioma.

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Data availability

All data generated or analysed during this study are included in this published article (and its supplementary information files).

Change history

  • 07 October 2020

    For the reference citation '[57]' in the second paragraph of the <Emphasis Type="Bold">Results</Emphasis> section of the original article there was no corresponding entry in the References section. It should have referred to the below mentioned article by Ebrahimkhani et al. (2018).

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Acknowledgements

We would like to thank Professor Mark Rosenthal for helpful discussions and comments regarding this work.

Funding

Cure Brain Cancer Foundation Australia, Brain Foundation Australia, The Royal Melbourne Hospital Neuroscience Foundation.

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Authors and Affiliations

  1. Department of Surgery, University of Melbourne, Parkville, VIC, Australia

    Andrew Morokoff, Jordan Jones, Hong Nguyen, Chenkai Ma, Iwan Bennett, Rod Luwor, Lucia Paradiso, Katharine Drummond & Andrew H. Kaye

  2. Department of Neurosurgery, Royal Melbourne Hospital, Parkville, VIC, Australia

    Andrew Morokoff, Jordan Jones, Stanley Stylli, Iddo Paldor & Katharine Drummond

  3. Department of Radiology, University of Melbourne, Parkville, VIC, Australia

    Arian Lasocki & Frank Gaillard

  4. Department of Medicine, University of Melbourne, Parkville, VIC, Australia

    Rachel Koldej

  5. Walter and Eliza Hall Institute, Parkville, VIC, Australia

    Ramyar Molania, Terence P. Speed, Andrew Webb & Guiseppe Infusini

  6. Peter MacCallum Cancer Centre, Parkville, VIC, Australia

    Jason Li

  7. Neuro-Oncology, Davidoff Institute of Oncology, Rabin Medical Center, Petach Tikva, Israel

    Tali Siegal

  8. Clinical Outcomes Research Unit, Royal Melbourne Hospital, Parkville, VIC, Australia

    Charles Malpas & Tomas Kalincik

  9. Department of Neurosurgery, Hadassah Hebrew University, Jerusalem, Israel

    Andrew H. Kaye

  10. Department of Surgery, University of Melbourne, Royal Melbourne Hospital Parkville, VIC, 3050, Australia

    Andrew Morokoff

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Contributions

Conception, planning: AM, AK, TS, KD. Writing & editing manuscript: AM, JJ, HN, KD, TS, FG, AK. Experimental work: HN, JJ, IB, RL, SS, RK, IP, LP, CM. Biostatistics: JL, AW, GI, CM, TS, RM, TK, CM. Radiology analysis: FG, AL, JJ.

Corresponding author

Correspondence to Andrew Morokoff.

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The authors declare no conflict of interest.

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Ethics approval for this study was obtained from the Melbourne Health Human Research Ethics Committee (HREC 2009.114), Melbourne, Australia.

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All patients provided written informed consent prior to inclusion. The study was performed in accordance with the Declaration of Helsinki.

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Morokoff, A., Jones, J., Nguyen, H. et al. Serum microRNA is a biomarker for post-operative monitoring in glioma. J Neurooncol 149, 391–400 (2020). https://doi.org/10.1007/s11060-020-03566-w

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