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Protective Effects of Flavonoids Against Oxidative Stress Induced by Simulated Microgravity in SH-SY5Y Cells

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Abstract

Many lines of evidence suggest that microgravity results in increased oxidative stress in the nervous system. In order to protect neuronal cells from oxidative damage induced by microgravity, we selected some flavonoids that might prevent oxidative stress because of their antioxidant activities. Among the 20 flavonoids we examined, we found that isorhamnetin and luteolin had the best protective effects against H2O2 or SIN-1-induced cytotoxicity in SH-SY5Y cells. Using a clinostat to simulate microgravity, we found that isorhamnetin and luteolin treatment protected SH-SY5Y cells by preventing microgravity-induced increases in reactive oxygen species (ROS), nitric oxide (NO) and 3-nitrotyrosine (3-NT) levels, and a decrease in antioxidant power (AP). Moreover, isorhamnetin and luteolin treatment downregulated the expression of inducible nitric oxide synthase (iNOS), and oxidative stress was significantly inhibited by an iNOS inhibitor in SH-SY5Y cells exposed to simulated microgravity (SMG). These results indicate that isorhamnetin and luteolin could protect against microgravity-induced oxidative stress in neuroblastoma SH-SY5Y cells by inhibiting the ROS-NO pathway. These two flavonoids may have potential for preventing oxidative stress induced by space flight or microgravity.

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Acknowledgments

We are grateful to Dr. Edward Sharman at the University of California at Irvine for his critical and careful reading and editing of the manuscript. This work was supported by the National Natural Science Foundation of China (Grant No. 30600759 and 30973686) and China Manned Space Advanced Research Foundation.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Health Science Center, Peking University, 38 Xueyuan Road, 100191, Beijing, China

    Lina Qu & Zebin Mao

  2. State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, Beiqing Road 26#, P.O. BOX 5132-23#, 100094, Haidian District, Beijing, China

    Lina Qu, Hailong Chen, Lei Bi, Jianghui Xiong & Yinghui Li

  3. Peking Union Medical College, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing, China

    Xinmin Liu

Authors
  1. Lina Qu
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  2. Hailong Chen
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  3. Xinmin Liu
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  4. Lei Bi
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  5. Jianghui Xiong
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  6. Zebin Mao
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Corresponding authors

Correspondence to Zebin Mao or Yinghui Li.

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Lina Qu and Hailong Chen contributed equally to this work.

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Qu, L., Chen, H., Liu, X. et al. Protective Effects of Flavonoids Against Oxidative Stress Induced by Simulated Microgravity in SH-SY5Y Cells. Neurochem Res 35, 1445–1454 (2010). https://doi.org/10.1007/s11064-010-0205-4

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  • DOI: https://doi.org/10.1007/s11064-010-0205-4

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