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Possible Mechanisms Involved in Attenuation of Lipopolysaccharide-Induced Memory Deficits by Methyl Jasmonate in Mice

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Abstract

This present study was carried out to investigate the likely mechanisms by which methyl jasmonate (MJ), ‘an agent widely used in aromatherapy for neurological disorders, attenuates lipopolysaccharide (LPS)-induced memory deficits in mice. Mice were given intraperitoneal administration of LPS (250 µg/kg) alone or in combination with MJ (10–40 mg/kg), donepezil, DP (1 mg/kg), or vehicle for 7 successive days. Thereafter, memory was assessed using object recognition test (ORT). Acetylcholinesterase and myeloperoxidase activities were estimated in brain tissue homogenates. Brain levels of nitric oxide and markers of oxidative stress as well as histopathologic changes of the prefrontal cortex and cornu ammonis 1 (CA1) of the hippocampal region were also assessed. MJ (10–40 mg/kg) attenuated LPS-induced memory impairment in ORT. Moreover, the increased brain activities of acetylcholinesterase and myeloperoxidase enzymes were suppressed by MJ when compared with control (p < 0.05). Increased brain oxidative stress and nitric oxide levels in LPS-treated mice were significantly decreased by MJ. It offers protection against LPS-induced neuronal degeneration of the prefrontal cortex and CA1 of the hippocampus, suggesting neuroprotective effect. Taken together, these findings showed that MJ offers protection against LPS-induced memory deficits via mechanisms related to inhibition of acetylcholinesterase, myeloperoxidase, oxidative stress and neuronal degeneration.

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Acknowledgments

Authors thanked the technical staff of the Department of Pharmacology and Therapeutics for their assistance. We also appreciate Professors E.A. Bababumi and O.G. Ademowo for introducing methyl jasmonate to us.

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Authors and Affiliations

  1. Neuropharmacology unit, Department of Pharmacology and Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria

    Anthony Taghogho Eduviere & Solomon Umukoro

  2. Department of Pharmacology and Therapeutics, College of Medicine and Health Sciences, Afe Babalola University, Ado-Ekiti, Nigeria

    Anthony Taghogho Eduviere & Olusegun A. Adeoluwa

  3. Department of Pharmacology and Therapeutics, College of Health Sciences, Delta State University, Abraka, Nigeria

    Itivere Adrian Omogbiya

  4. Department of Pharmacology and Therapeutics, College of Health Sciences, Bowen University, Iwo, Osun State, Nigeria

    Oritoke Modupe Aluko

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  1. Anthony Taghogho Eduviere
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Correspondence to Solomon Umukoro.

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Eduviere, A.T., Umukoro, S., Adeoluwa, O.A. et al. Possible Mechanisms Involved in Attenuation of Lipopolysaccharide-Induced Memory Deficits by Methyl Jasmonate in Mice. Neurochem Res 41, 3239–3249 (2016). https://doi.org/10.1007/s11064-016-2050-6

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  • DOI: https://doi.org/10.1007/s11064-016-2050-6

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