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The Arabidopsis MYB96 transcription factor plays a role in seed dormancy

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Abstract

Seed dormancy facilitates to endure environmental disadvantages by confining embryonic growth until the seeds encounter favorable environmental conditions for germination. Abscisic acid (ABA) and gibberellic acid (GA) play a pivotal role in the determination of the seed dormancy state. ABA establishes seed dormancy, while GA triggers seed germination. Here, we demonstrate that MYB96 contributes to the fine-tuning of seed dormancy regulation through the coordination of ABA and GA metabolism. The MYB96-deficient myb96-1 seeds germinated earlier than wild-type seeds, whereas delayed germination was observed in the activation-tagging myb96-1D seeds. The differences in germination rate disappeared after stratification or after-ripening. The MYB96 transcription factor positively regulates ABA biosynthesis genes 9-CIS-EPOXYCAROTENOID DIOXYGENASE 2 (NCED2), NCED5, NCED6, and NCED9, and also affects GA biosynthetic genes GA3ox1 and GA20ox1. Notably, MYB96 directly binds to the promoters of NCED2 and NCED6, primarily modulating ABA biosynthesis, which subsequently influences GA metabolism. In agreement with this, hyperdormancy of myb96-1D seeds was recovered by an ABA biosynthesis inhibitor fluridone, while hypodormancy of myb96-1 seeds was suppressed by a GA biosynthesis inhibitor paclobutrazol (PAC). Taken together, the metabolic balance of ABA and GA underlies MYB96 control of primary seed dormancy.

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Acknowledgments

This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2013R1A1A1004831). H.G.L. and K.L. were supported by the BK21 PLUS program in the Department of Bioactive Material Sciences.

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    1. Department of Bioactive Material Sciences and Research Center of Bioactive Materials, Chonbuk National University, Jeonju, 561-756, Republic of Korea

      Hong Gil Lee, Kyounghee Lee & Pil Joon Seo

    2. Department of Chemistry and Research Institute of Physics and Chemistry, Chonbuk National University, Jeonju, 561-756, Republic of Korea

      Pil Joon Seo

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    1. Hong Gil Lee
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    Hong Gil Lee and Kyounghee Lee have contributed equally to this work.

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    Lee, H.G., Lee, K. & Seo, P.J. The Arabidopsis MYB96 transcription factor plays a role in seed dormancy. Plant Mol Biol 87, 371–381 (2015). https://doi.org/10.1007/s11103-015-0283-4

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