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In vivo speciation of zinc in Noccaea caerulescens in response to nitrogen form and zinc exposure

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Abstract

Nitrate has been shown to enhance Zn hyperaccumulation in the shoots of Noccaea caerulescens (formerly Thlaspi caerulescens) (Prayon); however, the mechanisms beyond the effect of nitrogen form are unknown. This study used synchrotron X-ray absorption near-edge spectroscopy (XANES) on alive and intact plants at room temperature to examine whether enhanced Zn hyperaccumulation in nitrate-fed plants was associated with differences in Zn speciation, and to correlate Zn species with mechanisms of Zn uptake, translocation and hyperaccumulation. The higher Zn concentration in plants supplied with nitrate compared to ammonium, or with high Zn exposure (300 μΜ), was not due to differences in Zn speciation. The importance of carboxylates for Zn hyperaccumulation in the shoots was supported by a predominance of Zn-malate or Zn-citrate. Zinc-phytate was detected for the first time in this species and may assist Zn-tolerance in the roots. The feasible presence of Zn-histidine in the roots but not in the xylem sap suggests a mechanism for Zn binding and non-toxic transport through the cytoplasm and release of aqueous Zn into the xylem vessels. Zinc was translocated in the xylem as Zn-malate and weakly complexed or aqueous Zn forms. Zinc speciation in roots, shoots and xylem did not differ between nitrate- and ammonium-fed plants.

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Acknowledgements

This research was undertaken at the Australian National Beamline Facility (ANBF) at the Photon Factory in Japan, operated by the Australian Synchrotron. Dr Michael Cheah (ANBF) is gratefully acknowledged for support during the beamtime. We thank Giang Nguyen for her invaluable technical assistance in the laboratory. We also acknowledge the Linkage Infrastructure, Equipment and Facilities Program (LE0989759) and the Linkage Project (LP100100800) of the Australian Research Council for financial support, and the High Energy Accelerator Research Organisation (KEK) in Tsukuba, Japan, for operation support. Travel funding to the ANBF was kindly provided by the Australian Synchrotron Research Program (ASRP).

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  1. Department of Agricultural Science, La Trobe University, Melbourne Campus, Bundoora, Victoria, 3086, Australia

    Alison C. Monsant & Caixian Tang

  2. Centre for Materials and Surface Science and Department of Physics, La Trobe University, Bundoora, Victoria, 3086, Australia

    Peter Kappen & Paul J. Pigram

  3. School of Botany, University of Melbourne, Parkville, Victoria, 3000, Australia

    Yaodong Wang & Alan J. M. Baker

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  1. Alison C. Monsant
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Correspondence to Caixian Tang.

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Monsant, A.C., Kappen, P., Wang, Y. et al. In vivo speciation of zinc in Noccaea caerulescens in response to nitrogen form and zinc exposure. Plant Soil 348, 167–183 (2011). https://doi.org/10.1007/s11104-011-0887-7

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