Skip to main content
SpringerLink
Log in

Proteomic Analysis of Developing Somatic Embryos of Coffea arabica

  • Original Paper
  • Published:
Plant Molecular Biology Reporter Aims and scope Submit manuscript

Abstract

Differential protein profiles of three stages of somatic embryogenesis, including globular, torpedo, and cotyledonary somatic embryos, of Coffea arabica cv. Catuaí Vermelho were analyzed in an attempt to better understand somatic embryogenesis in coffee plants. Somatic embryos at these different stages of development were collected from in vitro-grown cultures, and then macerated in liquid nitrogen. Proteins were extracted with phenol and further quantified using the Bradford method. The bidimensional electrophoresis analysis revealed a wide range of proteins ranging between 10 and 160 kDa and of pH values ranging from 3 to 10. Several differentially expressed proteins were identified by mass spectrometry, and some were found to be specific to these different stages of somatic embryogenesis in coffee. The enolase and 11S storage globulin proteins, for example, could be used as molecular markers for somatic embryo development stages and for embryogenic and non-embryogenic genotype differentiation, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Andriotis VM, Kruger NJ, Pike MJ, Smith AM (2010) Plastidial glycolysis in developing Arabidopsis embryos. New Phytol 185:649–662

    Article  PubMed  CAS  Google Scholar 

  • Baba AI, Nogueira FCS, Pinheiro CB, Brasil JN, Jereissati ES, Jucá TL, Soares AA, Santos MF, Domont GB, Campos FAP (2008) Proteome analysis of secondary somatic embryogenesis in cassava (Manihot esculenta). Plant Sci 175:717–723

    Article  CAS  Google Scholar 

  • Bian F, Zheng C, Qu F, Gong X, You C (2010) Proteomic analysis of somatic embryogenesis in Cyclamen persicum Mill. Plant Mol Biol Rep 28:22–31

    Article  CAS  Google Scholar 

  • Blum H, Beier H, Gross HJ (1987) Improved silver staining of plant-proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8:93–99

    Article  CAS  Google Scholar 

  • Boxtel J, Berthouly M (1996) High frequency somatic embryogenesis from coffee leaves. Plant Cell Tissue Organ Cult 44:7–17

    Article  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities utilizing the principle of protein dye binding. Anal Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

  • Collet JF, Stroobant V, Van Schaftingen E (2001) The 2,3-bisphosphoglycerate-independent phosphoglycerate mutase from Trypanosoma brucei: metal-ion dependency and phosphoenzyme formation. FEMS Microbiol Lett 204:39–44

    Article  PubMed  CAS  Google Scholar 

  • de Mot R, Vanderleyden J (1989) Application of two dimensional protein analysis for strain fingerprinting and mutant analysis of Azospirillum species. Can J Microbiol 35:960–967

    Article  Google Scholar 

  • Fehér A, Pasternak TP, Dudits D (2003) Transition of somatic plant cells to an embryogenic state. Plant Cell Tissue Organ Cult 74:201–228

    Article  Google Scholar 

  • Gallardo K, Signor C, Vandekerckhove J, Thompson RD, Burstin J (2003) Proteomics of Medicago truncatula seed development establishes the time frame of diverse metabolic processes related to reserve accumulation. Plant Physiol 133:664–682

    Article  PubMed  CAS  Google Scholar 

  • Gerke V, Moss SE (2002) Annexins: from structure to function. Physiol Rev 82:331–371

    PubMed  CAS  Google Scholar 

  • Görg A, Postel W, Domscheit A, Günther S (1988) Two-dimensional electrophoresis with immobilized pH gradients of leaf proteins from barley (Hordeum vulgare ): method, reproducibility and genetic aspects. Electrophoresis 9:691–692

    Google Scholar 

  • Graña X, Ureña J, Ludevid D, Carreras J, Climent F (1989) Purification, characterization and immunological properties of 2,3-bisphosphoglycerate-independent phosphoglycerate mutase from maize (Zea mays) seeds. Eur J Biochem 186:149–153

    Article  PubMed  Google Scholar 

  • Hancock JT, Henson D, Nyirenda M, Desikan R, Harrison J, Lewis M, Hughes J, Neill SJ (2005) Proteomic identification of glyceraldehyde 3-phosphate dehydrogenase as an inhibitory target of hydrogen peroxide in Arabidopsis. Plant Physiol Biochem 43:828–883

    Article  PubMed  CAS  Google Scholar 

  • Huang X, Lu X-Y, Zhao J-T, Chen J-K, Dai X-M, Xiao W, Chen Y-P, Chen Y-F, Huang X-L (2010) MaSERK1 gene expression associated with somatic embryogenic competence and disease resistance response in banana (Musa spp.). Plant Mol Biol Rep 28:309–316

    Article  CAS  Google Scholar 

  • Kairong C, Gengsheng X, Xinmin L, Gengmei X, Yafu W (1999) Effect of hydrogen peroxide on somatic embryogenesis of Lycium barbarum L. Plant Sci 146:9–16

    Article  Google Scholar 

  • Karuna SB, Rajendrakumar CS, Reddy AR (2000) Aldose reductase in rice (Oryza sativa L.): stress response and developmental. Plant Sci 160:149–157

    Article  Google Scholar 

  • King JE, Gifford DJ (1997) Amino acid utilization in seeds of loblolly pine during germination and early seedling growth. Plant Physiol 113:1125–1135

    PubMed  CAS  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  PubMed  CAS  Google Scholar 

  • Li K, Zhu W, Zeng K, Zhang Z, Ye J, Ou W, Rehman S, Heuer B, Chen S (2010) Proteome characterization of cassava (Manihot esculenta Crantz) somatic embryos, plantlets and tuberous roots. Proteome Sci 8:10

    Article  PubMed  CAS  Google Scholar 

  • Lippert D, Zhuang J, Ralph S, Ellis DE, Gilbert M, Olafson R, Ritland K, Ellis B, Douglas CJ, Bohlmann J (2005) Proteome analysis of early somatic embryogenesis in Picea glauca. Proteomics 5:461–473

    Article  PubMed  CAS  Google Scholar 

  • Ma J, He Y, Wu C, Liu H, Hu Z, Sun G (2012) Cloning and molecular characterization of a SERK gene transcriptionally induced during somatic embryogenesis in Ananas comosus cv. Shenwan Plant Mol Biol Report 30:195–203

    Article  CAS  Google Scholar 

  • Marivet J, Margis-Pinheiro M, Frendo P, Burkard G (1994) Bean cyclophilin gene expression during plant development and stress conditions. Plant Mol Biol 26:1181–1189

    Article  PubMed  CAS  Google Scholar 

  • Mechin V, Thevenot C, Le Guilloux M, Prioul JL, Damerval C (2007) Developmental analysis of maize endosperm proteome suggests a pivotal role for pyruvate orthophosphate dikinase. Plant Physiol 143:1203–1219

    Article  PubMed  CAS  Google Scholar 

  • Moss SE, Morgan RO (2004) The annexins. Genome Biol 5:219.1–219.8

    Article  Google Scholar 

  • Pereira AR, de Carvalho SP, Pasqual M, Santos FC (2007) Embriogênese somática direta em explantes foliares de Coffea arabica l. cv. Acaiá Cerrado: efeito de cinetina e ácido giberélico. Ciênc Agrotec 31:332–336

    CAS  Google Scholar 

  • Ruan SL, Ma HS, Wang SH, Fu YP, Xin Y, Liu WZ, Wang F, Tong JX, Wang SZ, Chen HZ (2011) Proteomic identification of OsCYP2, a rice cyclophilin that confers salt tolerance in rice (Oryza sativa L.) seedlings when overexpressed. BMC Plant Biol 11:34

    Article  PubMed  CAS  Google Scholar 

  • Scholze C, Peterson A, Diettrich B, Luckner M (1999) Cyclophilin isoforms from Digitalis lanata: sequences and expression during embryogenesis and stress. J Plant Physiol 155: 212–219

    Google Scholar 

  • Sghaier-Hammami B, Drira D, Jorrín-Novo JV (2009) Comparative 2-DE proteomic analysis of date palm (Phoenix dactylifera L.) somatic and zygotic embryos. J Proteomics 73:161–177

    Article  PubMed  CAS  Google Scholar 

  • Sharifi G, Ebrahimzadeh H, Ghareyazie B, Gharechahi J, Vatankhah E (2012) Identification of differentially accumulated proteins associated with embryogenic and non-embryogenic calli in saffron (Crocus sativus L.). Proteome Sci 10:3

    Article  PubMed  CAS  Google Scholar 

  • Shevchenko A, Wilm M, Vorm O, Mann M (1996) Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal Chem 68:850–858

    Article  PubMed  CAS  Google Scholar 

  • Shewry PR, Napier JA, Tatham AS (1995) Seed storage proteins: structure and biosynthesis. Plant Cell 7:945–956

    PubMed  CAS  Google Scholar 

  • Thibaud-Nissen F, Shealy RT, Khanna A, Vodkin LO (2003) Clustering of microarray data reveals transcript patterns associated with somatic embryogenesis in soybean. Plant Physiol 132:118–136

    Article  PubMed  CAS  Google Scholar 

  • Van Der Straeten D, Rodriguespousada RA, Goodman HM, Van Montagu M (1991) Plant enolase-gene structure, expression, and evolution. Plant Cell 3:719–735

    PubMed  Google Scholar 

  • Vieira L, Andrade A, Colombo C, Moraes A, Mehta A et al (2006) Brazilian coffee genome project: an EST-based genomic resource. Braz J Plant Physiol 18:95–108

    Article  CAS  Google Scholar 

  • Wold F, Ballou CE (1957) Studies on the enzyme enolase. J Biol Chem 227:301–328

    PubMed  CAS  Google Scholar 

  • Yang C, Zhao T, Yu D, Gai J (2011) Isolation and functional characterization of a SERK gene from soybean (Glycine max (L.) Merr.) plant. Mol Biol Rep 29:334–344

    Article  CAS  Google Scholar 

  • Zeng F, Zhang X, Zhu L, Tu L, Guo X, Nie Y (2006) Isolation and characterization of genes associated to cotton somatic embryogenesis by suppression subtractive hybridization and macroarray. Plant Mol Biol 60:167–183

    Article  PubMed  CAS  Google Scholar 

  • Zhu JK, Shi J, Bressan RA, Hasegawa PM (1993) Expression Of An Atriplex nummularia gene encoding a protein homologous to the bacterial molecular chaperone DNA. J Plant Cell 5:341–349

    CAS  Google Scholar 

Download references

Acknowledgements

This research was supported by Consórcio Brasileiro de Pesquisa e Desenvolvimento do Café, Embrapa and UCB.

Author information

Authors and Affiliations

  1. Centro de Análises Proteômicas e Bioquímicas Pos-Graduação em Ciências Genômicas e Biotecnologia, Universidade Catolica de Brasilia, SGAN 916, Av. W5, Módulo C, sala 219, CEP 70790-160, Brasília, DF, Brazil

    Ângela Tonietto & Octávio Luiz Franco

  2. Universidade de Brasília, Campus Universitário Darcy Ribeiro, CEP 70910-900, Brasília, DF, Brazil

    Juliana Hiromi Sato

  3. Embrapa Recursos Genéticos e Biotecnologia, Avenida W 5 Norte Final, CEP 70770-917, Brasília, DF, Brazil

    João Batista Teixeira & Angela Mehta

  4. Universidade Federal do Paraná, Centro Politécnico, Caixa Postal 19046, CEP 81531-980, Curitiba, PR, Brazil

    Emanuel M. de Souza & Fabio O. Pedrosa

Authors
  1. Ângela Tonietto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Juliana Hiromi Sato
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. João Batista Teixeira
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Emanuel M. de Souza
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Fabio O. Pedrosa
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Octávio Luiz Franco
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Angela Mehta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Angela Mehta.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tonietto, Â., Sato, J.H., Teixeira, J.B. et al. Proteomic Analysis of Developing Somatic Embryos of Coffea arabica . Plant Mol Biol Rep 30, 1393–1399 (2012). https://doi.org/10.1007/s11105-012-0425-7

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11105-012-0425-7

Keywords

Search

Navigation