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Determination of Antioxidant Constituents in Cactus Pear Fruits

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Abstract

An analytical study was carried out on the presence of antioxidant constituents and the in vitro antioxidant capacity in the extracts of three species of Spanish red-skinned cactus pear fruits (Opuntia ficus-indica, Opuntia undulata and Opuntia stricta). The cactus pear fruit extracts were analyzed for determined constituents: ascorbic acid, flavonoids (quercetin, isorhamnetin, myricetin, kaempferol and luteolin), betalains, taurine, total carotenoids and total phenolics. The antioxidant capacity was assessed by means of two different methods: the 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (Trolox equivalent antioxidant capacity) method and the 2,2-diphenyl-1-picrylhydrazyl radical method. Opuntia ficus-indica fruit extract had the strongest antioxidant capacity and taurine content. O. stricta fruits were the richest in ascorbic acid and total phenolics, whereas O. undulata fruits showed the highest carotenoid content. Quercetin and isorhamnetin were the main flavonoids detected. This study provides basic information on the presence of bioactive compounds and antioxidant capacity in extracts of cactus pear fruits, in order to consider these extracts as ingredient for the production of health-promoting food.

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References

  1. Sáenz C (2000) Processing technologies: an alternative for cactus pear (Opuntia spp.) fruits and cladodes. J Arid Environ 46:209–225

    Article  Google Scholar 

  2. Stintzing FC, Schieber A, Carle R (2001) Phytochemical and nutritional significance of cactus pear. Eur Food Res Technol 212:396–407

    Article  CAS  Google Scholar 

  3. Feugang JM, Konarski P, Zou D, Stintzing FC, Zou C (2006) Nutritional and medicinal use of cactus pear (Opuntia spp.) cladodes and fruits. Front Biosci 11:2574–2589

    Article  CAS  Google Scholar 

  4. Hernández-Pérez T, Carrillo-López A, Guevara-Lara F, Cruz-Hernández A, Paredes-López O (2005) Biochemical and nutritional characterization of three prickly pear species with different ripening behaviour. Plant Foods Hum Nutr 60:195–200

    Article  Google Scholar 

  5. Castellar MR, Obón JM, Fernández-López JA (2006) The isolation and properties of a concentrated red-purple betacyanin food colourant from Opuntia stricta fruits. J Sci Food Agric 86:122–128

    Article  CAS  Google Scholar 

  6. Moßhammer MR, Stintzing FC, Carle R (2006) Evaluation of different methods for the production of juice concentrates and fruit powders from cactus pear. Innovat Food Sci Emerg Tech 7:275–287

    Article  Google Scholar 

  7. Tesoriere L, Fazzari M, Allegra M, Livrea MA (2005) Biothiols, taurine, and lipid-soluble antioxidants in the edible pulp of Sicilian cactus pear (Opuntia ficus-indica) fruits and changes of bioactive juice components upon industrial processing. J Agric Food Chem 53:7851–7855

    Article  CAS  Google Scholar 

  8. Arcoleo A, Ruccia M, Cusmano S (1961) Sui pigmenti flavonici delle Opuntiae. Nota 1. Isoramnetina dai fiori di O. ficus-indica Mill. Ann Chim 51:751–758

    CAS  Google Scholar 

  9. Galati EM, Mondello MR, Giuffrida D, Dugo G, Miceli N, Pergolizzi S, Taviano MF (2003) Chemical characterization and biological effects of Sicilian Opuntia ficus-indica (L.) Mill. fruit juice: antioxidant and antiulcerogenic activity. J Agric Food Chem 51:4903–4908

    Article  CAS  Google Scholar 

  10. Kuti JO (2004) Antioxidant compounds from four Opuntia cactus pear fruit varieties. Food Chem 85:527–533

    Article  CAS  Google Scholar 

  11. Santos-Buelga C, Scalbert A (2000) Proanthocyanidins and tannin-like compounds: nature, occurrence, dietary intake and effects on nutrition and health. J Sci Food Agric 80:1094–1117

    Article  CAS  Google Scholar 

  12. Manach C, Scalbert A, Morand C, Remesy C, Jiménez L (2004) Polyphenols: food sources and bioavailability. Am J Clin Nutr 79:727–747

    CAS  Google Scholar 

  13. Chávez-Santoscoy RA, Gutiérrez-Uribe JA, Serna-Saldívar SO (2009) Phenolic composition, antioxidant capacity and in vitro cancer cell cytotoxicity of nine prickly pear (Opuntia spp.) juices. Plant Foods Hum Nutr 64:146–152

    Article  Google Scholar 

  14. Zulueta A, Esteve MJ, Frígola A (2009) ORAC and TEAC assays comparison to measure the antioxidant capacity of food products. Food Chem 114:310–316

    Article  CAS  Google Scholar 

  15. Rice-Evans CA, Miller NJ, Papanga G (1997) Antioxidant properties of phenolic compounds. Trends Plant Sci 2:152–159

    Article  Google Scholar 

  16. Fernández-López JA, Castellar R, Obón JM, Almela L (2002) Screening and mass-spectral confirmation of betalains in cactus pears. Chromatographia 56:591–595

    Article  Google Scholar 

  17. Fernández-López JA, Castellar R, Obón JM, Almela L (2007) Monitoring by liquid chromatography coupled to mass spectrometry the impact of pH and temperature on the pigment pattern of cactus pear fruit extracts. J Chromatogr Sci 45:120–125

    Google Scholar 

  18. Castellar R, Obón JM, Alacid M, Fernández-López JA (2003) Color properties and stability of betacyanins from Opuntia fruits. J Agric Food Chem 51:2772–2776

    Article  CAS  Google Scholar 

  19. Obón JM, Castellar MR, Alacid M, Fernández-López JA (2009) Production of a red-purple food colorant from Opuntia stricta fruits by spray drying and its application in food model systems. J Food Eng 90:471–479

    Article  Google Scholar 

  20. Singh J, Upadhyay AK, Prasad K, Bahadur A, Rai M (2007) Variability of carotenes, vitamin C, E and phenolics in Brassica vegetables. J Food Compos Anal 20:106–112

    Article  CAS  Google Scholar 

  21. Rodríguez-Amaya DB (2001) A guide to carotenoid analysis in foods. ILSI Human Nutrition Institute Press, Washington DC

    Google Scholar 

  22. Obón JM, Castellar R, Cascales JA, Fernández-López JA (2005) Assessment of the TEAC method for determining the antioxidant capacity of synthetic red food colorants. Food Res Int 38:843–845

    Article  Google Scholar 

  23. Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. Lebens Wiss Technol 28:25–30

    CAS  Google Scholar 

  24. Díaz-Medina EM, Rodríguez-Rodríguez EM, Díaz-Romero C (2007) Chemical characterization of Opuntia dillenii and Opuntia ficus-indica fruits. Food Chem 103:38–45

    Article  Google Scholar 

  25. Gil MI, Tomás-Barberán FA, Hess-Pierce B, Kader AA (2002) Antioxidant capacities, phenolic compounds, carotenoids and vitamin C contents of nectarine, peach, and plum cultivars from California. J Agric Food Chem 50:4976–4982

    Article  CAS  Google Scholar 

  26. O’Neill ME, Carroll Y, Corridan B, Olmedilla B, Granado F, Blanco I, Van der Berg H, Hininger I, Rousell AM, Chopra M, Southon S, Thurnham DI (2001) A European carotenoid database to assess carotenoid intakes and its use in a five country comparative study. Br J Nutr 85:499–507

    Article  Google Scholar 

  27. Rao AV, Rao LG (2007) Carotenoids and human health. Pharmacol Res 55:207–216

    Article  CAS  Google Scholar 

  28. Miean KH, Mohamed S (2001) Flavonoid (myricetin, quercetin, kaempferol, luteolin and apigenin) content of edible tropical plants. J Agric Food Chem 49:3106–3112

    Article  CAS  Google Scholar 

  29. Stintzing FC, Herbach KM, Mosshammer MR, Carle R, Yi W, Sellappan S, Akoh CC, Bunch R, Felker P (2005) Color, betalain pattern, and antioxidant properties of cactus pear (Opuntia spp.) clones. J Agric Food Chem 53:442–451

    Article  CAS  Google Scholar 

  30. Tomás-Barberán FA, Gil MI, Cremin P, Waterhouse AI, Hess-Pierce B, Kader AA (2001) HPLC-DAD-ESI/MS analysis of phenolic compounds in nectarines, peaches and plums. J Agric Food Chem 49:4748–4760

    Article  Google Scholar 

  31. Toor RK, Savage GP (2005) Antioxidant activity in different fractions of tomatoes. Food Res Int 38:487–494

    Article  CAS  Google Scholar 

  32. Wójcik OP, Koenig KL, Zeleniuch-Jacquote A, Costa M, Chen Y (2010) The potential protective effects of taurine on coronary heart disease. Atherosclerosis 208:19–25

    Article  Google Scholar 

  33. Stintzing FC, Schieber A, Carle R (1999) Amino acid composition and betaxantin formation in fruits from Opuntia ficus-indica. Planta Med 65:632–635

    Article  CAS  Google Scholar 

  34. Prior RL, Wu X, Schaich K (2005) Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem 53:4290–4302

    Article  CAS  Google Scholar 

  35. Butera D, Tesoriere L, Di Gaudio F, Bongiorno A, Allegra M, Pintaudi AM, Kohen R, Livrea MA (2002) Antioxidant activities of Sicilian prickly pear (Opuntia ficus-indica) fruit extracts and reducing properties of its betalains: betanin and indicaxanthin. J Agric Food Chem 50:6895–6901

    Article  CAS  Google Scholar 

  36. Piga A, Del Caro A, Pinna I, Agabbio M (2003) Changes in ascorbic acid, polyphenol content and antioxidant activity in minimally processed cactus pear fruits. Lebensm Wiss Technol 36:257–262

    Article  CAS  Google Scholar 

  37. Kugler F, Stintzing FC, Carle R (2007) Evaluation of the antioxidant capacity of betalainic fruits and vegetables. J Appl Bot Food Qual 81:69–76

    CAS  Google Scholar 

Download references

Acknowledgements

This work was financially supported from the Department of Education and Science (MEC) of Spain, project AGL2004-01871. Valuable suggestions made by the reviewers are acknowledged.

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Correspondence to José A. Fernández-López.

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Fernández-López, J.A., Almela, L., Obón, J.M. et al. Determination of Antioxidant Constituents in Cactus Pear Fruits. Plant Foods Hum Nutr 65, 253–259 (2010). https://doi.org/10.1007/s11130-010-0189-x

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