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Flavoring Extracts of Hemidesmus indicus Roots and Vanilla planifolia Pods Exhibit In vitro Acetylcholinesterase Inhibitory Activities

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Abstract

Acetylcholinesterase inhibitors (AChEIs) are important for treatment of Alzheimer’s disease and other neurological disorders. Search for potent and safe AChEIs from plant sources still continues. In the present work, we explored fragrant plant extracts that are traditionally used in flavoring foods, namely, Hemidesmus indicus and Vanilla planifolia, as possible sources for AChEI. Root and pod extracts of H. indicus and V. planifolia, respectively, produce fragrant phenolic compounds, 2-hydroxy-4-methoxybenzaldehyde (MBALD) and 4-hydroxy-3-methoxybenzaldehyde (vanillin). These methoxybenzaldehydes were shown to have inhibitory potential against acetylcholinesterase (AChE). Vanillin (IC50 = 0.037 mM) was detected as more efficient inhibitor than MBALD (IC50 = 0.047 mM). This finding was supported by kinetic analysis. Thus, plant-based food flavoring agents showed capacity in curing Alzheimer’s disease and other neurological dysfunctions.

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Abbreviations

MBALD:

2-hydroxy-4-methoxybenzaldehyde

AChE:

Acetylcholinesterase

AChEI:

Acetylcholinesterase inhibitor

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Acknowledgements

This work was supported in part by a research grant (no. 2008/37/7/BRNS to A. Mitra) from the Board of Research in Nuclear Sciences (BRNS), Department of Atomic Energy, Government of India.

Conflict of Interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Natural Product Biotechnology Group, Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur, 721 302, India

    Anish Kundu & Adinpunya Mitra

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  1. Anish Kundu
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  2. Adinpunya Mitra
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Correspondence to Adinpunya Mitra.

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Kundu, A., Mitra, A. Flavoring Extracts of Hemidesmus indicus Roots and Vanilla planifolia Pods Exhibit In vitro Acetylcholinesterase Inhibitory Activities. Plant Foods Hum Nutr 68, 247–253 (2013). https://doi.org/10.1007/s11130-013-0363-z

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