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Rapid Solid-Liquid Dynamic Extraction (RSLDE): a New Rapid and Greener Method for Extracting Two Steviol Glycosides (Stevioside and Rebaudioside A) from Stevia Leaves

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Abstract

Stevioside and rebaudioside A are the main diterpene glycosides present in the leaves of the Stevia rebaudiana plant, which is used in the production of foods and low-calorie beverages. The difficulties associated with their extraction and purification are currently a problem for the food processing industries. The objective of this study was to develop an effective and economically viable method to obtain a high-quality product while trying to overcome the disadvantages derived from the conventional transformation processes. For this reason, extractions were carried out using a conventional maceration (CM) and a cyclically pressurized extraction known as rapid solid-liquid dynamic extraction (RSLDE) by the Naviglio extractor (NE). After only 20 min of extraction using the NE, a quantity of rebaudioside A and stevioside equal to 1197.8 and 413.6 mg/L was obtained, respectively, while for the CM, the optimum time was 90 min. From the results, it can be stated that the extraction process by NE and its subsequent purification developed in this study is a simple, economical, environmentally friendly method for producing steviol glycosides. Therefore, this method constitutes a valid alternative to conventional extraction by reducing the extraction time and the consumption of toxic solvents and favouring the use of the extracted metabolites as food additives and/or nutraceuticals. As an added value and of local interest, the experiment was carried out on stevia leaves from the Benevento area (Italy), where a high content of rebaudioside A was observed, which exhibits a sweet taste compared to stevioside, which has a significant bitter aftertaste.

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References

  1. Madan S, Ahmad S, Singh GN, Kohli K, Kumar Y, Singh R, Garg M (2010) Stevia rebaudiana (Bert.) Bertoni-a review. Indian J Nat Prod Resour 1(3):267–286

    CAS  Google Scholar 

  2. Singh A, Singh K, Singh P, Singh MP (2015) Medicinal prospective and floral biology of candy leaf (Stevia rebaudiana Bertoni). Int J 3(9):628–636

    Google Scholar 

  3. De S, Mondal S, Banerjee S (2013) Introduction to stevioside. Stevioside technology, applications and health. Wiley, pp 1–27. doi:10.1002/9781118350720.ch1

  4. Adesh AB, Gopalakrishna B, Kusum SA, Tiwari OP (2012) An overview on stevia: a natural calorie free sweetener. Int J Adv Pharm Biol Chem 1(3):362–368

  5. De Oliveira BH, Packer JF, Chimelli M, de Jesus DA (2007) Enzymatic modification of stevioside by cell-free extract of Gibberella fujikuroi. J Biotechnol 131(1):92–96

    Article  CAS  Google Scholar 

  6. Brandle JE, Starratt AN, Gijzen M (1998) Stevia rebaudiana: its agricultural, biological, and chemical properties. Can J Plant Sci 78(4):527–536

    Article  CAS  Google Scholar 

  7. Goyal SK, Goyal RK (2010) Stevia (Stevia rebaudiana) a bio-sweetener: a review. Int J Food Sci Nutr 61(1):1–10

    Article  CAS  Google Scholar 

  8. Logue C, Peters SJ, Gallagher AM, Verhagen H (2015) Perspectives on low calorie intense sweeteners with a focus on aspartame and stevia. Eur J Food Res Rev 5(2):104–112

    Google Scholar 

  9. Tosun J (2013) Stevia-based sweeteners as a food additive. In: Risk Regulation in Europe. Springer, New York, pp. 83–95

  10. Periche A, Castelló ML, Heredia A, Escriche I (2015) Influence of extraction methods on the yield of steviol glycosides and antioxidants in Stevia rebaudiana extracts. Plant Foods Hum Nutr 70:119–127

    Article  CAS  Google Scholar 

  11. Gasmalla MAA, Yang R, Hua X (2014) Stevia rebaudiana Bertoni: an alternative sugar replacer and its application in food industry. Food Eng Rev 6(4):150–162

    Article  CAS  Google Scholar 

  12. Rao AB, Prasad E, Roopa G, Sridhar S, Ravikumar YVL (2012) Simple extraction and membrane purification process in isolation of steviosides with improved organoleptic activity. Adv Biosci Biotechnol 3(4):327–335

    Article  CAS  Google Scholar 

  13. De S, Mondal S, Banerjee S (2013) State of the art of stevioside processing using membrane-based filtration. In: Stevioside: Technology, Applications and Health. John Wiley & Sons. Oxford, pp 91–98. doi:10.1002/9781118350720.ch6

  14. Rao AB, George SA, Alavala S, Meshram H, Mand Shekar KC (2015) Metal salts assisted enzyme-based extraction of stevioside from the leaves of Stevia rebaudiana Bertoni. Adv Biosci Biotechnol 6(12):734–743

    Article  CAS  Google Scholar 

  15. Carbonell-Capella JM, Šic Žlabur J, Rimac S et al (2016) Electrotechnologies, microwaves, and ultrasounds combined with binary mixtures of ethanol and water to extract steviol glycosides and antioxidant compounds from Stevia rebaudiana leaves. J Food Process Preserv e13179. doi:10.1111/jfpp.13179

  16. Koubaa M, Roselló-Soto E, Šic Žlabur J et al (2015) Current and new insights in the sustainable and green recovery of nutritionally valuable compounds from Stevia rebaudiana Bertoni. J Agric Food Chem 63(31):6835–6846

    Article  CAS  Google Scholar 

  17. Barba FJ, Grimi N, Vorobiev E (2015) Evaluating the potential of cell disruption technologies for green selective extraction of antioxidant compounds from Stevia rebaudiana Bertoni leaves. J Food Eng 149:222–228

    Article  CAS  Google Scholar 

  18. Barba F, Grimi N, Negm M et al. (2014) Green recovery technology of sweeteners from Stevia rebaudiana Bertoni leaves. In: Leaf sweeteners: resources, processing and health effects, pp. 41–55

  19. Roselló-Soto E, Galanakis CM, Brnčić M et al (2015) Clean recovery of antioxidant compounds from plant foods, by-products and algae assisted by ultrasounds processing. Modeling approaches to optimize processing conditions. Trends Food Sci Technol 42(2):134–149

    Article  Google Scholar 

  20. Barba FJ, Criado MN, Belda-Galbis CM et al (2014) Stevia rebaudiana Bertoni as a natural antioxidant/antimicrobial for high pressure processed fruit extract: processing parameter optimization. Food Chem 148:261–267

    Article  CAS  Google Scholar 

  21. Barba FJ, Parniakov O, Pereira SA et al (2015) Current applications and new opportunities for the use of pulsed electric fields in food science and industry. Food Res Int 77:773–798

    Article  Google Scholar 

  22. Naviglio D (2003) Naviglio's principle and presentation of an innovative solid–liquid extraction technology: extractor Naviglio®. Anal Lett 36(8):1647–1659

    Article  CAS  Google Scholar 

  23. Naviglio D, Formato A, Gallo M (2014) Comparison between 2 methods of solid–liquid extraction for the production of Cinchona calisaya elixir: an experimental kinetics and numerical modeling approach. J Food Sci 79(9):1704–1712

    Article  Google Scholar 

  24. Naviglio D, Montesano D, Gallo M (2015) Laboratory production of lemon liqueur (Limoncello) by conventional maceration and a two-syringe system to illustrate rapid solid–liquid dynamic extraction. J Chem Educ 92(5):911–915

    Article  CAS  Google Scholar 

  25. Wölwer-Rieck U (2012) The leaves of Stevia rebaudiana (Bertoni), their constituents and the analyses thereof: a review. J Agric Food Chem 60(4):886–895

    Article  Google Scholar 

  26. Anton S, Martin C, Han H, Coulon S, Cefalu W, Geiselman P et al (2010) Effects of stevia, aspartame, and sucrose on food intake, satiety and postprandial glucose and insulin levels. Appetite 55:37–43

    Article  CAS  Google Scholar 

  27. Ferrazzano GF, Cantile T, Alcidi B, Coda M et al (2016) Is Stevia rebaudiana Bertoni a non cariogenic sweetener? A review. Molecules 21(1):38. doi:10.3390/molecules21010038

    Article  Google Scholar 

  28. Yadav AK, Singh S, Dhyani D, Ahuja PS (2011) A review on the improvement of stevia [Stevia rebaudiana (Bertoni)]. Can J Plant Sci 91(1):1–27

    Article  Google Scholar 

  29. Deshmukh SR, Kedari VR (2014) Isolation, purification and characterization of sweetners from Stevia rebaudiana (Bertoni) for their anticancerous activity against colon cancer. World J Pharm Pharm Sci 3(5):1394–1410

    Google Scholar 

  30. Lemus-Mondaca R, Vega-Gálvez A, Zura-Bravo L, Ah-Hen K (2012) Stevia rebaudiana Bertoni, source of a high-potency natural sweetener: a comprehensive review on the biochemical, nutritional and functional aspects. Food Chem 132(3):1121–1132

    Article  CAS  Google Scholar 

  31. Puri M, Sharma D, Tiwari AK (2011) Downstream processing of stevioside and its potential applications. Biotechnol Adv 29(6):781–791

    Article  CAS  Google Scholar 

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Acknowledgements

This study was supported by the Campania Region that funded the project DoDiS (Sweet Stevia of Sannio) and acknowledges Coldiretti Benevento as the leader of the project (PSR Campania 2007-2013, Misura 124 Progetto “Do.di.S - Dolci di Stevia del Sannio” DRD n.99 del 17/06/2014, CUP: B76G14000290006).

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Authors and Affiliations

  1. Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, via Pansini, 5, 80131, Naples, Italy

    Monica Gallo

  2. Department of Chemical Sciences, University of Naples Federico II, via Cintia, 80126, Naples, Italy

    Manuela Vitulano, Anna Andolfi, Marina DellaGreca, Esterina Conte, Martina Ciaravolo & Daniele Naviglio

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  1. Monica Gallo
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  2. Manuela Vitulano
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  3. Anna Andolfi
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  4. Marina DellaGreca
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  5. Esterina Conte
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  6. Martina Ciaravolo
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  7. Daniele Naviglio
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Correspondence to Monica Gallo.

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Gallo, M., Vitulano, M., Andolfi, A. et al. Rapid Solid-Liquid Dynamic Extraction (RSLDE): a New Rapid and Greener Method for Extracting Two Steviol Glycosides (Stevioside and Rebaudioside A) from Stevia Leaves. Plant Foods Hum Nutr 72, 141–148 (2017). https://doi.org/10.1007/s11130-017-0598-1

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  • DOI: https://doi.org/10.1007/s11130-017-0598-1

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