Skip to main content
SpringerLink
Log in

Micropropagation of Gymea Lily (Doryanthes excelsa Corrêa) from New South Wales, Australia

  • Original Paper
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

The physiological effects of three auxins [indole-3-butyric acid (IBA), α-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-d)] and two cytokinins [thidiazuron (TDZ) and N6-benzylaminopurine (NAA)] on in vitro morphogenesis of Doryanthes excelsa were measured. Longitudinal bud sections derived from immature inflorescences were used as a source of explants. Callus regeneration was observed at the highest frequencies (46.2%) when grown on media containing 50 μmol L-1 NAA and 0.5 μmol L−1 TDZ. Adventitious shoot organogenesis was observed at the highest frequency (56.8%) when grown on media containing 0.5 μmol L−1 NAA and 50 μmol L−1 TDZ. Regenerated shoots were rooted ex vitro after 6 weeks when dipped in a solution of 50 μmol L−1 NAA or no plant growth regulators were applied.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

Abbreviations

2,4-d :

2,4-dichlorophenoxyacetic acid

BA:

N6-benzylaminopurine

IBA:

Indole−3-butyric acid

MES:

n-morpholinoethanesulfonic acid

MS:

Murashige and Skoog (1962) medium

NAA:

α-naphthaleneacetic acid

TDZ:

Thidiazuron [1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea]

References

  • Boltenkov EV, Rybin VG, Zarembo EV (2004) Specific features of cultivation of Iris ensata thunb. Callus tissue. Appl Microbiol Biotechnol 40(2):206–212

    Article  CAS  Google Scholar 

  • Boltenkov EV, Zarembo EV (2005) In vitro regeneration and callogenesis in tissue culture of floral organs of the genus Iris (Iridaceae). Biol Bull 32(2):138–142

    Article  Google Scholar 

  • Burchett M, Nash S, Richardson N (1989) Doryanthes excelsa as a commercial crop. Aust Hortic 87(9):36–39

    Google Scholar 

  • Ellyard RK (1978) In vitro propagation of Anigozanthos manglesii, Anigozanthus flavidus and Macropidia fuliginosa. HortScience 13(6):662–663

    Google Scholar 

  • Fay MF, Rudall PJ, Sullivan S, Stobart KL, De Bruijn AY, Reeves G, Qamaruz-Zaman F, Hong W-P, Joseph J, Hahn WJ, Conran JG, Chase MW (2000) Phylogenetic studies of asparagales based on four plastid DNA regions. Monocots: systematics and evolution. In: Wilson KL, Morrison DA (eds). Commonwealth Scientific and Industrial Research Organisation (CSIRO) Publishing, Melbourne, Vic., Australia

    Google Scholar 

  • Genkov T, Tsoneva P, Ivanova I (1997) Effect of cytokinins on photosynthtic pigments and chlorophyllase activity in in vitro cultures of axillary buds of Dianthus caryophyllus L. J Plant Growth Regul 16:169–172

    Article  CAS  Google Scholar 

  • Gorst JR (1996) Tissue culture of Australian monocotyledons including the orchids. In: Taji A, Williams R (eds). Tissue culture of Australian plants. University of New England Printery, Armidale, NSW, Australia, pp 56–111

    Google Scholar 

  • Greisbach RJ (1989) Selection of dwarf Hemerocallis through tissue culture. HortScience 24(6):1027–1028

    Google Scholar 

  • Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ 33:105–119

    Article  CAS  Google Scholar 

  • Johnson KA (2000) Induction of somatic embryogenesis in Australian monocots. Acta Hortic 520:67–74

    Google Scholar 

  • Johnson KA, Burchett M (1991) In vitro propagation of Blandfordia grandiflora (Liliaceae). J Hortic Sci 66(4):389–394

    Google Scholar 

  • Mok MC, Mok DWS (1985) The metabolism of [14C]-thidiazuron in callus tissues of Phaseolus lunatus. Physiol Plant 65:427–432

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Murthy BNS, Murch SJ, Savxena PK (1998) Thidiazuron: a potent regulator of in vitro plant morphogenesis. In Vitro Cell Dev Plant 34:267–275

    CAS  Google Scholar 

  • Newman IV (1928) The life history of Doryanthes excelsa: part 1: some ecological and vegetative features and spore production. In: Proceedings of the Linnaean Society of New South Wales, vol 53. pp499–528

  • Ramsey JL, Galitz DS, Lee CW (2003) Basal medium and sucrose concentration influence regeneration of Easter Lily in ovary culture. HortScience 38(3):404–406

    Google Scholar 

  • Ricci A, Carra A, Torelli A, Maggiali CA, Vicini P, Zani F, Branca C (2001) Cytokinin-like activity of N’-substituted N-phenylureas. Plant Growth Regul 34:167–172

    Article  CAS  Google Scholar 

  • Shibli RA, Ajlouni MM (2000) Somatic embryogenesis in the endemic black iris. Plant Cell Tissue Organ 61(1):15–21

    Article  CAS  Google Scholar 

  • Shimizu K, Nagaike H, Yabuya T, Adachi T (1997) Plant regeneration from suspension culture of Iris germanica. Plant Cell Tissue Organ 50:27–31

    Article  Google Scholar 

  • Torres KC (1989) Tissue culture techniques for horticultural crops, edn. AVI-Van Nostrand New York, NY, USA

    Google Scholar 

  • Velcheva M, Faltin Z, Vardi A, Eshdat Y, Perl A (2005) Regeneration of Aloë arborescens via somatic organogenesis from young inflorescences. Plant Cell Tissue Organ 83:293–301

    Article  Google Scholar 

  • Visser C, Fletcher RA, Saxena PK (1995) TDZ stimulates expansion and greening in cucumber cotyledons. Physiol Mol Biol Plants 1:21–26

    Google Scholar 

Download references

Acknowledgements

A. M. Dimech wishes to thank Mr. Greg Miles of Princeton Nurseries (Mount White, NSW, Australia) for his generous assistance. This research was jointly funded by the Rural Industries Research and Development Corporation and the Commonwealth Government of Australia through an Australian Postgraduate Award.

Author information

Authors and Affiliations

  1. Royal Botanic Gardens Melbourne, South Yarra, 3141, Vic., Australia

    Adam M. Dimech & Rob Cross

  2. Faculty of Land and Food Resources, University of Melbourne, Royal Parade, 3010, VIC, Australia

    Adam M. Dimech, Rebecca Ford & Paul W. J. Taylor

Authors
  1. Adam M. Dimech
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rob Cross
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rebecca Ford
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Paul W. J. Taylor
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Adam M. Dimech.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dimech, A.M., Cross, R., Ford, R. et al. Micropropagation of Gymea Lily (Doryanthes excelsa Corrêa) from New South Wales, Australia. Plant Cell Tiss Organ Cult 88, 157–165 (2007). https://doi.org/10.1007/s11240-006-9184-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-006-9184-1

Keywords

Search

Navigation