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A Passiflora homolog of a D-type cyclin gene is differentially expressed in response to sucrose, auxin, and cytokinin

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Abstract

In higher plants, one of the major components of developmental processes is cell division. The cell division cycle in plants is controlled by cyclins and cyclin-dependend kinases. Nutrient and hormonal signals can influence the roles that D-type cyclins play in the G1-to-S phase transition. Auxins and cytokinins are long known to be important plant hormones controlling plant growth. Additionally, as sucrose is the major transported carbon source in higher plants, it is possible that it plays a major role in cell division. To access the molecular aspects of the effect of auxin, cytokinin and sucrose on the regulation of cell cycle machinery and plant development, we cloned a Passiflora morifolia putative homolog to a D-type cyclin, PmCYCD1, which showed high sequence similarity to other known plant D-type cyclins. We examined the expression patterns of PmCYCD1 during callus induction and growth in in vitro conditions. We observed incremented expression levels of PmCYCD1 correlated to increasing concentrations of sucrose, α-naphthalene acetic acid and 6-benzyladenine in the culture medium. Additionally, the results of in situ hybridization experiments indicated a dynamic spatial expression pattern for PmCYCD1 during callus growth.

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Acknowledgments

To Prof EW Kitajima and Prof F Tanaka for maintaining the electron microscope facility at NAP/MEPA-ESALQ/USP. To Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil), Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, São Paulo, Brazil) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil) for funding.

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  1. Faculdade de Ciências Agrárias, Universidade Federal da Grande Dourados, Rod. Dourados-Itaum, km 12, Cx Postal 533, Dourados, MS, CEP 79804-970, Brazil

    Yara Brito Chaim Jardim Rosa

  2. Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, R. Monteiro Lobato, 255, Cx Postal 6109, Campinas, SP, CEP 13418-970, Brazil

    Lílian Cristina Baldon Aizza, Jaderson Silveira Leite Armanhi & Marcelo Carnier Dornelas

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  1. Yara Brito Chaim Jardim Rosa
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Correspondence to Marcelo Carnier Dornelas.

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Rosa, Y.B.C.J., Aizza, L.C.B., Armanhi, J.S.L. et al. A Passiflora homolog of a D-type cyclin gene is differentially expressed in response to sucrose, auxin, and cytokinin. Plant Cell Tiss Organ Cult 115, 233–242 (2013). https://doi.org/10.1007/s11240-013-0355-6

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